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Bone fracture in a rat femoral fracture model is associated with the activation of autophagy

  • Authors:
    • Qiankun Zhou
    • Deqing Luo
    • Teng Li
    • Zhirong Liu
    • Weitao Zou
    • Lei Wang
    • Dasheng Lin
    • Kejian Lian
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Affiliated Southeast Hospital of Xiamen University, Orthopedic Center of People's Liberation Army, Zhangzhou, Fujian 363000, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1675-1680
    |
    Published online on: September 17, 2015
       https://doi.org/10.3892/etm.2015.2752
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Abstract

Autophagy, which is a mechanism for the turnover of intracellular molecules and organelles, protects cells during stress responses; however, the role of autophagy in the stages of bone fracture remains to be elucidated. The aim of the present study was to investigate the process of autophagy in bone tissue at different time‑points after fracture. A femur fracture model was established in male adult Wistar rats via surgery. The protein expression of microtubule‑associated protein II light chain 3 (LC3‑II) was analyzed in a femur fracture (experimental) group and a sham‑surgery group using immunofluorescence. The protein expression of proliferating cell nuclear antigen (PCNA) was used to investigate the cell proliferation in bone tissue following fracture via immunohistochemical analysis. The correlation between cell proliferation and autophagy was analyzed using linear regression. LC3‑II protein was constitutively expressed in the sham‑surgery group; however, compared with the expression in the sham‑surgery group, the LC3‑II expression in the experimental group was significantly increased at each time‑point (P<0.05). Similarly, immunohistochemistry revealed that the number of PCNA‑positive cells in each section was significantly increased following fracture injury (P<0.01). A comparison of the LC3‑II‑ and PCNA‑positive rates in the experimental group rats at each time‑point revealed a linear correlation (R2=0.43, P<0.01). In conclusion, surgically induced fracture in rats is associated with an increase in LC3‑II and PCNA protein expression during the initial stages of fracture injury, and a correlation exists between the expression of the two proteins. These results suggest that potential treatment aimed at improving fracture healing should target the process of autophagy.
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1 

Megas P: Classification of non-union. Injury. 36(Suppl 4): S30–S37. 2005.PubMed/NCBI

2 

Rueff-Barroso CR, Milagres D, Valle J, Casimiro-Lopes G, Nogueira-Neto JF, Zanier JF and Porto LC: Bone healing in rats submitted to weight-bearing and non-weight-bearing exercises. Med Sci Monit. 14:BR231–BR236. 2008.PubMed/NCBI

3 

Mizushima N, Levine B, Cuervo AM and Klionsky DJ: Autophagy fights disease through cellular self-digestion. Nature. 451:1069–1075. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Levine B and Kroemer G: Autophagy in the pathogenesis of disease. Cell. 132:27–42. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Lin Y, Tang C, He H and Duan R: Activation of mTOR ameliorates fragile X premutation rCGG repeat-mediated neurodegeneration. PloS One. 8:e625722013. View Article : Google Scholar : PubMed/NCBI

6 

Su H, Li J, Osinska H, Li F, Robbins J, Liu J, Wei N and Wang X: The COP9 signalosome is required for autophagy, proteasome-mediated proteolysis, and cardiomyocyte survival in adult mice. Circ Heart Fail. 6:1049–1057. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Caramés B, Hasegaa A, Taniguchi N, Miyaki S, Blanco FJ and Lotz M: Autophagy activation by rapamycin reduces severity of experimental osteoarthritis. Ann Rheum Dis. 71:575–581. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Vellai T: Autophagy genes and ageing. Cell Death Differ. 16:94–102. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Klionsky DJ and Emr SD: Autophagy as a regulated pathway of cellular degradation. Science. 290:1717–1721. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Thorburn A: Apoptosis and autophagy: Regulatory connections between two supposedly different processes. Apoptosis. 13:1–9. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Bao XH, Naomoto Y, Hao HF, Watanabe N, Sakurama K, Noma K, Motoki T, Tomono Y, Fukazawa T, Shirakawa Y, et al: Autophagy: Can it become a potential therapeutic target? Int J Mol Med. 25:493–503. 2010.PubMed/NCBI

12 

Beau I, Mehrpour M and Codogno P: Autophagosomes and human diseases. Int J Biochem Cell Biol. 43:460–464. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Mijaljica D, Prescott M and Devenish RJ: Autophagy in disease. Methods Mol Biol. 648:79–92. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Zhang L, Guo YF, Liu YZ, Liu YJ, Xiong DH, Liu XG, Wang L, Yang TL, Lei SF, Guo Y, et al: Pathway-based genome-wide association analysis identified the importance of regulation-of-autophagy pathway for ultradistal radius BMD. J Bone Miner Res. 25:1572–1580. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Daroszewska A, van't Hof RJ, Rojas JA, Layfield R, Landao-Basonga E, Rose L, Rose K and Ralston SH: A point mutation in the ubiquitin-associated domain of SQSMT1 is sufficient to cause a Paget's disease-like disorder in mice. Hum Mol Genet. 20:2734–2744. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T, Kominami E, Ohsumi Y and Yoshimori T: LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19:5720–5728. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Su JC, Tseng PH, Hsu CY, Tai WT, Huang JW, Ko CH, Lin MW, Liu CY, Chen KF and Shiau CW: RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget. 5:4909–4919. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Manolagas SC: Birth and death of bone cells: Basic regulatory mechanisms and implications for the pathogenesis and treatment of osteoporosis. Endocr Rev. 21:115–137. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Lee FY, Choi YN, Beherns FF, Defoun DO and Einhorn TA: Programmed removal of chondrocytes during endochondral fracture healing. J Orthop Res. 16:144–150. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Einhorn TA: The cell and molecular biology of fracture dealing. Clin Orthop Relat Res. (355 Suppl): S7–S21. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Holstein JH, Menger MD, Culemann U, Meier C and Pohlemann T: Development of a locking femur nail for mice. J Biomech. 40:215–219. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Müller E, Koch P, Nazarian S and Schatzker J: The Comprehensive Classification of Fractures of Long Bones (1st). New York, NY: Springer. 1994.

23 

Hou H, Zhang L, Zhang L, Liu D, Xiong Q, Du H and Tang P: Acute spinal cord injury could cause activation of autophagy in dorsal root ganglia. Spinal Cord. 51:679–682. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Moscat J and Diaz-Meco MT: p62 at the crossroads of autophagy, apoptosis and cancer. Cell. 137:1001–1004. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Dosenko VE, Nagibin VS, Tumanovska LV and Moibenko AA: Protective effect of autophagy in anoxia-reoxygenation of isolated cardiomyocyte? Autophagy. 2:305–306. 2006. View Article : Google Scholar : PubMed/NCBI

26 

Mortimore GE and Schworer CM: Induction of autophagy by amino-acid deprivation in perfused rat liver. Nature. 270:174–176. 1977. View Article : Google Scholar : PubMed/NCBI

27 

Quan W, Jung HS and Lee MS: Role of autophagy in the progression from obesity to diabetes and in the control of energy balance. Arch Pharm Res. 36:223–229. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Barnouti ZP, Owtad P, Shen G, Petocz P and Darendeliler MA: The biological mechanisms of PCNA and BMP in TMJ adaptive remodeling. Angle Orthod. 81:91–99. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Iwaki A, Jingushi S, Oda Y, Izumi T, Shida JI, Tsuneyoshi M and Sugioka Y: Localization and quantification of proliferating cells during rat fracture repair: Detection of proliferating cell nuclear antigen by immunohistochemistry. J Bone Miner Res. 12:96–102. 1997. View Article : Google Scholar : PubMed/NCBI

30 

Morrow PW, Tung HY and Hemmings HC Jr: Rapamycin causes activation of protein phosphatase-2A1 and nuclear translocation of PCNA in CD4+ T cells. Biochem Biophys Res Commun. 323:645–651. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Kobayashi S, Kishimoto T, Kamata S, Otsuka M, Miyazaki M and Ishikura H: Rapamycin, a specific inhibitor of the mammalian target of rapamycin, suppresses lymphangiogenesis and lymphatic metastasis. Cancer Sci. 98:726–733. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Kawahara T, Asthana S and Kneteman NM: m-TOR inhibitors: What role in liver transplantation? J Hepatol. 55:1441–1451. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou Q, Luo D, Li T, Liu Z, Zou W, Wang L, Lin D and Lian K: Bone fracture in a rat femoral fracture model is associated with the activation of autophagy. Exp Ther Med 10: 1675-1680, 2015.
APA
Zhou, Q., Luo, D., Li, T., Liu, Z., Zou, W., Wang, L. ... Lian, K. (2015). Bone fracture in a rat femoral fracture model is associated with the activation of autophagy. Experimental and Therapeutic Medicine, 10, 1675-1680. https://doi.org/10.3892/etm.2015.2752
MLA
Zhou, Q., Luo, D., Li, T., Liu, Z., Zou, W., Wang, L., Lin, D., Lian, K."Bone fracture in a rat femoral fracture model is associated with the activation of autophagy". Experimental and Therapeutic Medicine 10.5 (2015): 1675-1680.
Chicago
Zhou, Q., Luo, D., Li, T., Liu, Z., Zou, W., Wang, L., Lin, D., Lian, K."Bone fracture in a rat femoral fracture model is associated with the activation of autophagy". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1675-1680. https://doi.org/10.3892/etm.2015.2752
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Q, Luo D, Li T, Liu Z, Zou W, Wang L, Lin D and Lian K: Bone fracture in a rat femoral fracture model is associated with the activation of autophagy. Exp Ther Med 10: 1675-1680, 2015.
APA
Zhou, Q., Luo, D., Li, T., Liu, Z., Zou, W., Wang, L. ... Lian, K. (2015). Bone fracture in a rat femoral fracture model is associated with the activation of autophagy. Experimental and Therapeutic Medicine, 10, 1675-1680. https://doi.org/10.3892/etm.2015.2752
MLA
Zhou, Q., Luo, D., Li, T., Liu, Z., Zou, W., Wang, L., Lin, D., Lian, K."Bone fracture in a rat femoral fracture model is associated with the activation of autophagy". Experimental and Therapeutic Medicine 10.5 (2015): 1675-1680.
Chicago
Zhou, Q., Luo, D., Li, T., Liu, Z., Zou, W., Wang, L., Lin, D., Lian, K."Bone fracture in a rat femoral fracture model is associated with the activation of autophagy". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1675-1680. https://doi.org/10.3892/etm.2015.2752
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