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Article

Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression

  • Authors:
    • Xiaodong Lu
    • Lufang Chen
    • Yuanyuan Chen
    • Qixiang Shao
    • Wenxin Qin
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, P.R. China, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai 200032, P.R. China
  • Pages: 1829-1834
    |
    Published online on: September 22, 2015
       https://doi.org/10.3892/etm.2015.2758
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Abstract

The vacuolar H+-ATPase (V-ATPase) is commonly highly activated in cancer cells and is a potential target of anti‑cancer therapy. Bafilomycin A1 is a specific inhibitor of the c subunit of V‑ATPase. In the present study, the effects of bafilomycin A1 on the BEL‑7402 hepatocellular carcinoma and HO‑8910 ovarian cancer cell lines were respectively studied. In addition, the bafilomycin A1‑induced alterations in the mRNAs and microRNAs (miRNAs) in the cells were detected using microarray methods. The results demonstrated that the growth of the two cell lines was retarded and the metastatic potential was inhibited by bafilomycin A1. Transmission electron microscopy and assays of capsase‑3 and ‑9 suggested that bafilomycin A1 induced apoptosis. Gene Ontology analysis of the microarrays of mRNA‑miRNA integrity showed altered pathways following bafilomycin A1 treatment, including pathways regulating glucose or lipid metabolism, DNA repair or duplication and lysosomes. Quantitative polymerase chain reaction analysis confirmed that miR‑923, miR‑1246, miR‑149*, miR‑638 and miR‑210 were upregulated and miR‑99a, miR‑181a‑2* and miR‑339‑5p were downregulated following bafilomycin A1 treatment. The overlapped altered miRs may be effective targets for the two types of solid tumor, and may have potential for application to the treatment of other types of solid tumor.
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Copy and paste a formatted citation
Spandidos Publications style
Lu X, Chen L, Chen Y, Shao Q and Qin W: Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression. Exp Ther Med 10: 1829-1834, 2015.
APA
Lu, X., Chen, L., Chen, Y., Shao, Q., & Qin, W. (2015). Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression. Experimental and Therapeutic Medicine, 10, 1829-1834. https://doi.org/10.3892/etm.2015.2758
MLA
Lu, X., Chen, L., Chen, Y., Shao, Q., Qin, W."Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression". Experimental and Therapeutic Medicine 10.5 (2015): 1829-1834.
Chicago
Lu, X., Chen, L., Chen, Y., Shao, Q., Qin, W."Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1829-1834. https://doi.org/10.3892/etm.2015.2758
Copy and paste a formatted citation
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Spandidos Publications style
Lu X, Chen L, Chen Y, Shao Q and Qin W: Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression. Exp Ther Med 10: 1829-1834, 2015.
APA
Lu, X., Chen, L., Chen, Y., Shao, Q., & Qin, W. (2015). Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression. Experimental and Therapeutic Medicine, 10, 1829-1834. https://doi.org/10.3892/etm.2015.2758
MLA
Lu, X., Chen, L., Chen, Y., Shao, Q., Qin, W."Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression". Experimental and Therapeutic Medicine 10.5 (2015): 1829-1834.
Chicago
Lu, X., Chen, L., Chen, Y., Shao, Q., Qin, W."Bafilomycin A1 inhibits the growth and metastatic potential of the BEL-7402 liver cancer and HO-8910 ovarian cancer cell lines and induces alterations in their microRNA expression". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1829-1834. https://doi.org/10.3892/etm.2015.2758
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