Open Access

Traditional Chinese medicine Qili qiangxin inhibits cardiomyocyte apoptosis in rats following myocardial infarction

  • Authors:
    • Jun Xiao
    • Song‑Bai Deng
    • Qiang She
    • Jun Li
    • Guo‑Ying Kao
    • Jun‑Sheng Wang
    • Yu Ma
  • View Affiliations

  • Published online on: September 22, 2015     https://doi.org/10.3892/etm.2015.2759
  • Pages: 1817-1823
  • Copyright: © Xiao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine the effect of the traditional Chinese medicine Qili qiangxin on cardiomyocyte apoptosis following myocardial infarction (MI) in a rat model. MI was induced in rats by ligation of the anterior descending coronary artery. Survivors were randomly divided into the sham operation, MI, and Qili qiangxin groups (4 g/kg per day). After 28 days, infarction size was measured. In the non‑infarcted zones (NIZ), the apoptotic index (AI) was measured by terminal deoxynucleotidyl transferase (TdT)‑mediated digoxigenin‑conjugated dUTP nick‑end labeling (TUNEL). Expression of Fas was detected by immunohistochemistry, and the expression of xanthine oxidase (XO) and caspase‑3 by western blot analysis. In addition, the XO and ·O2‑, ·OH‑scavenging activity of myocardial tissue in NIZ was measured by colorimetry. Compared to the MI group, AI and the expression of Fas and caspase‑3 were significantly decreased in NIZ. The activity of XO was also considerably reduced while ·O2‑ and ·OH‑scavenging activity was significantly increased in the Qili qiangxin group. Ventricular remodeling was attenuated but there were no significant differences in infarct size (IS) or XO expression levels between the Qili qiangxin and MI groups. In conclusion, the results suggest that Qili qiangxin may inhibit cardiomyocyte apoptosis in NIZ in rats. The potential mechanism involved may be associated with its ability to reduce reactive oxygen species (ROS) and to depress the expression of Fas and caspase-3.
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November-2015
Volume 10 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Xiao J, Deng SB, She Q, Li J, Kao GY, Wang JS and Ma Y: Traditional Chinese medicine Qili qiangxin inhibits cardiomyocyte apoptosis in rats following myocardial infarction. Exp Ther Med 10: 1817-1823, 2015
APA
Xiao, J., Deng, S., She, Q., Li, J., Kao, G., Wang, J., & Ma, Y. (2015). Traditional Chinese medicine Qili qiangxin inhibits cardiomyocyte apoptosis in rats following myocardial infarction. Experimental and Therapeutic Medicine, 10, 1817-1823. https://doi.org/10.3892/etm.2015.2759
MLA
Xiao, J., Deng, S., She, Q., Li, J., Kao, G., Wang, J., Ma, Y."Traditional Chinese medicine Qili qiangxin inhibits cardiomyocyte apoptosis in rats following myocardial infarction". Experimental and Therapeutic Medicine 10.5 (2015): 1817-1823.
Chicago
Xiao, J., Deng, S., She, Q., Li, J., Kao, G., Wang, J., Ma, Y."Traditional Chinese medicine Qili qiangxin inhibits cardiomyocyte apoptosis in rats following myocardial infarction". Experimental and Therapeutic Medicine 10, no. 5 (2015): 1817-1823. https://doi.org/10.3892/etm.2015.2759