Quantitative analysis of contrast-enhanced ultrasonography in acute radiation-induced liver injury: An animal model
- Authors:
- Jun Feng
- Shu-Bo Chen
- Shu-Jun Wu
- Ping Sun
- Tian-You Xin
- Ying-Zhen Chen
View Affiliations
Affiliations: Department of Ultrasound, The Second Hospital of Wuxi Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China, Department of Oncology, The Second Hospital of Wuxi Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China, Department of Pathology, The Second Hospital of Wuxi Affiliated to Nanjing Medical University, Wuxi, Jiangsu 214002, P.R. China
- Published online on: September 22, 2015 https://doi.org/10.3892/etm.2015.2764
-
Pages:
1807-1811
-
Copyright: © Feng
et al. This is an open access article distributed under the
terms of Creative
Commons Attribution License.
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
Abstract
The aim of the present study was to examine and assess contrast-enhanced ultrasound in the early diagnosis of acute radiation-induced liver injury in a rat model. Sixty female rats were used, with 50 rats being utilized to produce an animal model of liver injury with a single dose of stereotactic X-ray irradiation of 20 Gy. Ten rats from the injury group and 2 rats from the control group were randomly selected on days 3, 7, 14, 21 and 28, and examined by contrast-enhanced ultrasound and histopathology of liver specimens. The rats were divided into four groups: the normal control group, mild, moderate, and severe radioactive liver injury groups based on the histopathological examination results. Hepatic artery arriving time (HAAT) and hepatic vein arriving time (HVAT) were recorded, and hepatic artery to vein transit time (HA-HVTT) was calculated. The time-intensity curve of liver parenchyma, the time to peak (TTP) and peak intensity (PI) were also obtained. Significant differences were observed between liver injury and control groups for PI and HA-HVTT (P<0.05). PI and HA-HVTT were shorter in the severe liver injury group compared to the mild and moderate liver injury groups (P<0.05). Compared to the control group, higher TTP was recorded in all the liver injury groups (P<0.05), and the highest TTP level was observed in the severe liver injury group compared to the mild or moderate group (P<0.05). However, no significant difference was observed between the mild and moderate groups for PI, HA-HVTT and TTP. In conclusion, the results showed that contrast-enhanced ultrasonography is useful for an earlier diagnosis in a rat model of acute radiation-induced liver injury.
View References
|
1
|
Rahbari NN, Mehrabi A, Mollberg NM, Müller
SA, Koch M, Büchler MW and Weitz J: Hepatocellular carcinoma:
Current management and perspectives for the future. Ann Surg.
253:453–469. 2011. View Article : Google Scholar : PubMed/NCBI
|
|
2
|
Bujold A and Dawson LA: Stereotactic
radiation therapy and selective internal radiation therapy for
hepatocellular carcinoma. Cancer Radiother. 15:54–63. 2011.
View Article : Google Scholar : PubMed/NCBI
|
|
3
|
Peng RY, Wang DW, Xu ZH, et al: Dynamic
observation of the process of radiation-induced liver fibrosis.
Chin J Radiol Med Prot. 14:243–245. 1994.(In Chinese).
|
|
4
|
Fajardo LF: The pathology of ionizing
radiation as defined by morphologic patterns. Acta Oncol. 44:13–22.
2005. View Article : Google Scholar : PubMed/NCBI
|
|
5
|
Liang SX, Zhu XD, Lu HJ, Pan CY, Li FX,
Huang QF, Wang AY, Chen L, Fu XL and Jiang GL: Hypofractionated
three-dimensional conformal radiation therapy for primary liver
carcinoma. Cancer. 103:2181–2188. 2005. View Article : Google Scholar : PubMed/NCBI
|
|
6
|
Li N, Ding H, Lin XY, Fan PL, Xu C and
Wang WP: Correlations between quantitative parameters of
contrast-enhanced ultrasonography and histopathologic stage of
liver fibrosis. Chin J Ultrason. 18:942–945. 2009.(In Chinese).
|
|
7
|
Zhang L, Duan YY, Zhang Y, et al: Primary
research on diagnosis of liver fibrosis stage by perfusion dynamics
of ultrasound contrast agent. Chin J Ultrason. 16:616–619. 2007.(In
Chinese).
|
|
8
|
Jie LM, Guo QY, Liu X, et al: The
experimental study on the evaluation of liver fibrosis using
contrast enhanced sonography. Chin J Ultrason. 17:809–812. 2008.(In
Chinese).
|
|
9
|
Shen JK, Jiang Z, Zhou J, et al:
Experimental study on the early effects of radiation induced liver
injury: the number and the function evaluation of Kupffer cells.
Chin J Radiol Med Prot. 6:5532004.(In Chinese).
|
|
10
|
Zhou YX: The Modern Diagnosis and
Treatment of Liver Cirrhosis. Beijing: People's Medical Publishing
House. 142000.
|
|
11
|
Lu X, Xu GF, Chen WH, et al: The dynamic
changes of hepatic sinusoid capillarization during the formation of
hepatic fibrosis in rats. Shi Jie Hua Ren Xiao Hua Za Zhi.
8:1415–1416. 2000.(In Chinese).
|
|
12
|
Li R and Hua X: Experimental study on the
diagnostic value of the appearing time of ultrasound contrast agent
in hepatic vein in early liver cirrhosis. Chongqing Med J.
33:1684–1685. 2004.
|
|
13
|
Wang CP and Han J: Cellular and molecular
mechanism of portal hypertension. Infect Dis Info. 18:117–119.
2005.
|
|
14
|
Tan XQ, Qian LX, Zhang XL, et al:
Contrast-enhanced ultrasound in diagnosis of early hepatic
cirrhosis. Chin J Ultrason. 17:1048–1050. 2008.(In Chinese).
|
