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Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis

  • Authors:
    • Cong Feng
    • Bei Li
    • Li‑Li Wang
    • Li Chen
    • Xuan Zhou
    • Fa‑Qin Lv
    • Tan‑Shi Li
  • View Affiliations / Copyright

    Affiliations: Department of Emergency, PLA General Hospital, Beijing 100853, P.R. China, Department of Ultrasound, PLA General Hospital, Beijing 100853, P.R. China
    Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2029-2034
    |
    Published online on: October 15, 2015
       https://doi.org/10.3892/etm.2015.2802
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Abstract

The aim of the present study was to investigate the effect of peritoneal lavage with ulinastatin on the expression levels of nuclear factor κB (NF-κB) and tumor necrosis factor (TNF)‑α in multiple organs of rats with severe acute pancreatitis (SAP). Male Wistar rats were randomly divided into the following groups: Sham‑operated (C), SAP model (SAP), saline lavage (SL), intravenous ulinastatin (IU) and peritoneal lavage with ulinastatin (UL). The SAP model was induced by the retrograde infusion of 5% sodium taurocholate into the biliopancreatic ducts of the rats. Intraperitoneal lavage or injection was performed immediately following the establishment of the SAP model in groups SL, IU and UL. Intraperitoneal lavage with or without ulinastatin was performed for 3 h. The survival time of half of the rats in each group was recorded over a 12‑h period. At 3 h after the induction of SAP, inflammatory mediators and the expression levels of NF‑κB and TNF‑α in multiple organs of the rats in each group were also detected. The survival rates of the rats in group UL at 6 h and 9 h were increased compared with those in group SAP, and were also higher than that in groups SL and IU. The levels of serum inflammatory mediators were effectively reduced in groups SL, IU and UL, the greatest effects were observed in group UL. The expression levels of NF‑κB and TNF‑α in multiple organs were significantly lower in group UL compared with other groups. Intraperitoneal lavage with ulinastatin significantly ameliorated the inflammatory reaction and inhibited NF-κB and TNF-α expression in multiple organs of SAP model rats.
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Copy and paste a formatted citation
Spandidos Publications style
Feng C, Li B, Wang LL, Chen L, Zhou X, Lv FQ and Li TS: Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis. Exp Ther Med 10: 2029-2034, 2015.
APA
Feng, C., Li, B., Wang, L., Chen, L., Zhou, X., Lv, F., & Li, T. (2015). Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis. Experimental and Therapeutic Medicine, 10, 2029-2034. https://doi.org/10.3892/etm.2015.2802
MLA
Feng, C., Li, B., Wang, L., Chen, L., Zhou, X., Lv, F., Li, T."Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis". Experimental and Therapeutic Medicine 10.6 (2015): 2029-2034.
Chicago
Feng, C., Li, B., Wang, L., Chen, L., Zhou, X., Lv, F., Li, T."Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2029-2034. https://doi.org/10.3892/etm.2015.2802
Copy and paste a formatted citation
x
Spandidos Publications style
Feng C, Li B, Wang LL, Chen L, Zhou X, Lv FQ and Li TS: Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis. Exp Ther Med 10: 2029-2034, 2015.
APA
Feng, C., Li, B., Wang, L., Chen, L., Zhou, X., Lv, F., & Li, T. (2015). Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis. Experimental and Therapeutic Medicine, 10, 2029-2034. https://doi.org/10.3892/etm.2015.2802
MLA
Feng, C., Li, B., Wang, L., Chen, L., Zhou, X., Lv, F., Li, T."Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis". Experimental and Therapeutic Medicine 10.6 (2015): 2029-2034.
Chicago
Feng, C., Li, B., Wang, L., Chen, L., Zhou, X., Lv, F., Li, T."Effect of peritoneal lavage with ulinastatin on the expression of NF-κB and TNF-α in multiple organs of rats with severe acute pancreatitis". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2029-2034. https://doi.org/10.3892/etm.2015.2802
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