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Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment

  • Authors:
    • Yuntian Shen
    • Quanbin Zhang
    • Jinshi Zhang
    • Zhaohui Lu
    • Aidong Wang
    • Xifeng Fei
    • Xingliang Dai
    • Jinding Wu
    • Zhimin Wang
    • Yaodong Zhao
    • Ye Tian
    • Jun Dong
    • Qing Lan
    • Qiang Huang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Radiotherapy and Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, P.R. China, Department of Neurosurgery, Shanghai Tenth People's Hospital, Shanghai 200072, P.R. China, Department of Neurosurgery, Ganzhou People’s Hospital, Ganzhou, Jiangxi 341000, P.R. China, Department of Neurosurgery, Suzhou Kowloon Hospital, Suzhou, Jiangsu 215002, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2047-2054
    |
    Published online on: October 22, 2015
       https://doi.org/10.3892/etm.2015.2821
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Abstract

Various organs of the body have distinct microenvironments with diverse biological characteristics that can influence the growth of tumors within them. However, the mechanisms underlying the interactions between tumor and host cells are currently not well understood. In the present study, a dual‑color fluorescence‑tracing glioma orthotopic implantation model was developed, in which C6 rat glioma cells labeled with the red fluorescent dye CM‑Dil, and SU3 human glioma cells stably expressing red fluorescence protein, were inoculated into the right caudate nucleus of transgenic female C57BL/6 nude mice expressing enhanced green fluorescent protein. The dual‑color tracing with whole‑body in vivo fluorescence imaging of xenografts was performed using a live imaging system. Frozen sections of the transplanted tumor were prepared for histological analyses, in order to detect the presence of invading tumor cells, blood vessels and cellular fusion. Dual‑color images were able to distinguish between red tumor cells and green host cells. The results of the present study suggested that a dual‑color fluorescence‑tracing glioma orthotopic implantation model may be convenient for detecting tumor location, angiogenesis, cellular fusion, and the tumor microenvironment.
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Copy and paste a formatted citation
Spandidos Publications style
Shen Y, Zhang Q, Zhang J, Lu Z, Wang A, Fei X, Dai X, Wu J, Wang Z, Zhao Y, Zhao Y, et al: Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment. Exp Ther Med 10: 2047-2054, 2015.
APA
Shen, Y., Zhang, Q., Zhang, J., Lu, Z., Wang, A., Fei, X. ... Huang, Q. (2015). Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment. Experimental and Therapeutic Medicine, 10, 2047-2054. https://doi.org/10.3892/etm.2015.2821
MLA
Shen, Y., Zhang, Q., Zhang, J., Lu, Z., Wang, A., Fei, X., Dai, X., Wu, J., Wang, Z., Zhao, Y., Tian, Y., Dong, J., Lan, Q., Huang, Q."Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment". Experimental and Therapeutic Medicine 10.6 (2015): 2047-2054.
Chicago
Shen, Y., Zhang, Q., Zhang, J., Lu, Z., Wang, A., Fei, X., Dai, X., Wu, J., Wang, Z., Zhao, Y., Tian, Y., Dong, J., Lan, Q., Huang, Q."Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2047-2054. https://doi.org/10.3892/etm.2015.2821
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Y, Zhang Q, Zhang J, Lu Z, Wang A, Fei X, Dai X, Wu J, Wang Z, Zhao Y, Zhao Y, et al: Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment. Exp Ther Med 10: 2047-2054, 2015.
APA
Shen, Y., Zhang, Q., Zhang, J., Lu, Z., Wang, A., Fei, X. ... Huang, Q. (2015). Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment. Experimental and Therapeutic Medicine, 10, 2047-2054. https://doi.org/10.3892/etm.2015.2821
MLA
Shen, Y., Zhang, Q., Zhang, J., Lu, Z., Wang, A., Fei, X., Dai, X., Wu, J., Wang, Z., Zhao, Y., Tian, Y., Dong, J., Lan, Q., Huang, Q."Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment". Experimental and Therapeutic Medicine 10.6 (2015): 2047-2054.
Chicago
Shen, Y., Zhang, Q., Zhang, J., Lu, Z., Wang, A., Fei, X., Dai, X., Wu, J., Wang, Z., Zhao, Y., Tian, Y., Dong, J., Lan, Q., Huang, Q."Advantages of a dual-color fluorescence-tracing glioma orthotopic implantation model: Detecting tumor location, angiogenesis, cellular fusion and the tumor microenvironment". Experimental and Therapeutic Medicine 10, no. 6 (2015): 2047-2054. https://doi.org/10.3892/etm.2015.2821
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