Suppression of SIPA‑1 expression may reduce bladder cancer invasion and metastasis via the downregulation of E‑cadherin and ZO‑1 Corrigendum in /10.3892/etm.2022.11622

Zhang,Ping; Wang,Xinghuan

doi:10.3892/etm.2015.2891

Suppression of SIPA‑1 expression may reduce bladder cancer invasion and metastasis via the downregulation of E‑cadherin and ZO‑1

Corrigendum in: /10.3892/etm.2022.11622

Authors:
- Ping Zhang
- Xinghuan Wang
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Affiliations: Department of Urology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: November 24, 2015 https://doi.org/10.3892/etm.2015.2891
Pages: 213-217

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Abstract

The aim of the present study was to investigate the capacity of signal‑induced proliferation‑associated protein 1 (SIPA‑1) to regulate bladder cancer cell invasion and metastasis. BIU‑87 and T24 cells were transfected with the SIPA gene and SIPA short hairpin (sh)RNA, respectively. Western blot analysis was conducted to analyze the expression levels of SIPA‑1, Ras‑related protein 1 (Rap1), Rap1 guanosine triphosphate (Rap1GTP), E‑cadherin and zona occludens‑1 (ZO‑1). Cell motility and invasion were evaluated in vitro using wound and Transwell assays. Transfected cells were inoculated into the pelvic region of BALB/c nude mice, and the number of resulting tumors was recorded after 6 weeks. Western blot analysis revealed that expression levels of E‑cadherin and ZO‑1 were reduced in the cells with enhanced levels of SIPA‑1. By contrast, the levels of E‑cadherin and ZO‑1 were elevated in the cells in which SIPA‑1 was knocked down. In comparison with untransfected cells, the cells with reduced levels of SIPA‑1 exhibited reduced wound closure and fewer cells crossed the chamber in the Transwell experiment, whereas the cells with enhanced levels of SIPA‑1 exhibited increased migration and invasion In vivo, an increased tumor count was obtained in the mice with elevated levels of SIPA‑1. Therefore, the results of the present study indicate that SIPA‑1 is able to regulate bladder cancer cell metastasis and invasion by reducing the expression of E‑cadherin and ZO‑1.

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