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Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning

  • Authors:
    • Yi‑Hua Fa
    • Jian‑Qiang Ni
    • Xiao‑Jin Wu
    • Jia‑Qing Tan
    • Yi‑Wei Wu
  • View Affiliations / Copyright

    Affiliations: School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, Jiangsu 215000, P.R. China, Department of Neurology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Hematology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China, Department of Nuclear Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China
    Copyright: © Fa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 101-109
    |
    Published online on: November 27, 2015
       https://doi.org/10.3892/etm.2015.2900
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Abstract

The aim of the present study was to investigate the use of 18F-fallypride micro‑positron emission tomography (micro-PET) imaging in the evaluation of the early therapeutic efficacy of L-dopa in the treatment of Parkinson's disease (PD) and the underlying mechanism. 18F-fallypride was synthesized and its specific binding with dopamine (DA) receptors in normal mouse brain was studied. Following the establishment of a mouse model of PD, the animals were divided into normal control, PD model and L‑dopa treatment groups. General behavior, swimming test, locomotor activity counts, transmission electron microscopy, immunohistochemical analysis, high performance liquid chromatography-electrochemical detection and 18F‑fallypride micro‑PET imaging were used to study intergroup differences and the correlation between the changes of striatal uptake of 18F‑fallypride and the therapeutic efficacy. The general behavioral features of PD model mice were similar to the clinical symptoms of PD patients and were alleviated after treatment. The swimming time, locomotor activity and frequency of standing posture of PD model mice were lower than those of the control mice, but had no difference from those of the control mice after L‑dopa treatment. The number of tyrosine hydroxylase‑positive neurons and the striatal contents of glutathione peroxidase, superoxide dismutase, DA and its metabolites 3,5-dihydroxyphenylacetic acid and homovanillic acid in the PD group were lower than those in the control group, but were significantly improved following the treatment; the significant reduction in DOPAC/DA and HVA/DA ratios post treatment suggested that the rate of DA metabolism decreased significantly. The striatal malondialdehyde content in the PD group increased compared with that in the control group, but was reduced after L‑dopa treatment. Micro‑PET imaging indicated that the uptake of 18F‑fallypride in the mouse striatum of the PD group was lower than that of the control group and was significantly increased after the treatment. The mechanism of treatment of PD with L‑dopa in mice may involve increasing the number of TH-positive cells and DA receptor levels, as well as reducing the rate of DA metabolism; such changes can be noninvasively observed in vitro by 18F-fallypride imaging.
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Copy and paste a formatted citation
Spandidos Publications style
Fa YH, Ni JQ, Wu XJ, Tan JQ and Wu YW: Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning. Exp Ther Med 11: 101-109, 2016.
APA
Fa, Y., Ni, J., Wu, X., Tan, J., & Wu, Y. (2016). Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning. Experimental and Therapeutic Medicine, 11, 101-109. https://doi.org/10.3892/etm.2015.2900
MLA
Fa, Y., Ni, J., Wu, X., Tan, J., Wu, Y."Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning". Experimental and Therapeutic Medicine 11.1 (2016): 101-109.
Chicago
Fa, Y., Ni, J., Wu, X., Tan, J., Wu, Y."Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning". Experimental and Therapeutic Medicine 11, no. 1 (2016): 101-109. https://doi.org/10.3892/etm.2015.2900
Copy and paste a formatted citation
x
Spandidos Publications style
Fa YH, Ni JQ, Wu XJ, Tan JQ and Wu YW: Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning. Exp Ther Med 11: 101-109, 2016.
APA
Fa, Y., Ni, J., Wu, X., Tan, J., & Wu, Y. (2016). Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning. Experimental and Therapeutic Medicine, 11, 101-109. https://doi.org/10.3892/etm.2015.2900
MLA
Fa, Y., Ni, J., Wu, X., Tan, J., Wu, Y."Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning". Experimental and Therapeutic Medicine 11.1 (2016): 101-109.
Chicago
Fa, Y., Ni, J., Wu, X., Tan, J., Wu, Y."Evaluation of the early response and mechanism of treatment of Parkinson's disease with L-dopa using 18F-fallypride micro-positron emission tomography scanning". Experimental and Therapeutic Medicine 11, no. 1 (2016): 101-109. https://doi.org/10.3892/etm.2015.2900
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