Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th1, Th2, Th17 and Treg cells following thoracotomy of patients with lung cancer

Wu,Huaxing; Wang,Kun; Li,Guixian; Meng,Dexin; Han,Jiacheng; Wang,Guonian; Li,Yu

doi:10.3892/etm.2015.2913

February-2016 Volume 11 Issue 2

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February-2016 Volume 11 Issue 2

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Article

Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th1, Th2, Th17 and Treg cells following thoracotomy of patients with lung cancer

Authors:
- Huaxing Wu
- Kun Wang
- Guixian Li
- Dexin Meng
- Jiacheng Han
- Guonian Wang
- Yu Li
View Affiliations / Copyright

Affiliations: School of Life Science and Technology, Harbin Institute of Technology, Harbin, Heilongjiang 150080, P.R. China, Department of Anesthesiology, Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China, Department of Mechanical Design and Theory, School of Mechatronics Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, P.R. China, Department of Clinical Oncology, Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
Pages: 495-502
|
Published online on: December 4, 2015

https://doi.org/10.3892/etm.2015.2913
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Abstract

An imbalance in the various T lymphocytes, including T-helper (Th)1, Th2 and Th17 cells, and regulatory T (Treg) cells, has been associated with immune dysfunction, and may occur following thoracotomy of patients with lung cancer. The use of transcutaneous acupoint electrical stimulation (TAES) has previously been demonstrated to exert immunoregulatory effects; therefore, the present study aimed to evaluate whether TAES was able to attenuate postoperative immune suppression in patients with lung cancer. Thoracic surgical patients with lung cancer (n=27) underwent TAES (frequency, 2/100 Hz; intensity, 4‑12 mA) at the bilateral large intestine 4, pericardium 6, small intestine 3 and San Jiao 6 acupuncture points for 30 min, prior to incision, and at 20, 44, 68, 92 and 116 h following thoracotomy. The number of Th1, Th2, Th17 and Treg cells, and the protein and mRNA expression levels of related cytokines were measured by flow cytometry, ELISA and polymerase chain reaction, respectively. The balance of Th1, Th2, Th17 and Treg cells in the peripheral blood of patients with lung cancer was disrupted following thoracotomy. TAES administration increased the percentage of Th1 and Th17 cells, the protein expression levels of interleukin (IL)‑2 and interferon‑γ, the mRNA expression levels of T‑bet and RAR‑related orphan receptor‑γt, and decreased the percentage of Th2 cells, IL‑10 protein expression levels, and GATA binding protein 3 mRNA expression levels. The results of the present study demonstrated that TAES was able to partially attenuate the postoperative immune depression of patients with lung cancer, by regulating the balance of Th1, Th2, Th17 and Treg cells, and the expression levels of related cytokines and transcription factors; therefore, TAES may be considered to be a promising strategy for treating postoperative immune dysfunction in patients with lung cancer.

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1	Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI
2	Dasanu CA, Sethi N and Ahmed N: Immune alterations and emerging immunotherapeutic approaches in lung cancer. Expert Opin Biol Ther. 12:923–937. 2012. View Article : Google Scholar : PubMed/NCBI
3	Micheli DC, Fernandes PC Jr, Cruvinel JC, Nomelini ID, Murta EF and Tavares-Murta BM: Circulating cytokines and nitric oxide are involved in the Inhibition of Neutrophil Migration in patients with Uterine Cervical Neoplasia. Clin Med Insights Oncol. 6:233–242. 2012.PubMed/NCBI
4	Bobocea AC, Trandafir B, Bolca C and Cordoş I: Minimally invasive surgery in cancer. Histopathology. Chirurgia (Bucur). 107:154–157. 2012.PubMed/NCBI
5	Leaver HA, Craig SR, Yap PL and Walker WS: Lymphocyte responses following open and minimally invasive thoracic surgery. Eur J Clin Invest. 30:230–238. 2000. View Article : Google Scholar : PubMed/NCBI
6	Ren XF, Li WZ, Meng FY and Lin CF: Differential effects of propofol and isoflurane on the activation of T-helper cells in lung cancer patients. Anaesthesia. 65:478–482. 2010. View Article : Google Scholar : PubMed/NCBI
7	Dai H, Sun T, Liu Z, Zhang J and Zhou M: The imbalance between regulatory and IL-17-secreting CD4⁺T cells in multiple-trauma rat. Injury. 44:1521–1527. 2013. View Article : Google Scholar : PubMed/NCBI
8	Kong QF, Sun B, Bai SS, Zhai DX, Wang GY, Liu YM, Zhang SJ, Li R, Zhao W, Sun YY, et al: Administration of bone marrow stromal cells ameliorates experimental autoimmune myasthenia gravis by altering the balance of Th1/Th2/Th17/Treg cell subsets through the secretion of TGF-beta. J Neuroimmunol. 207:83–91. 2009. View Article : Google Scholar : PubMed/NCBI
9	Ye J, Liu H, Zhang G, Li P, Wang Z, Huang S, Yang Q and Li Y: The treg/th17 imbalance in patients with obstructive sleep apnoea syndrome. Mediators Inflamm. 2012:8153082012. View Article : Google Scholar : PubMed/NCBI
10	Yang L, Yang L and Gao X: Transcutaneous electrical nerve stimulation on Yongquan acupoint reduces CFA-induced thermal hyperalgesia of rats via down-regulation of ERK2 phosphorylation and c-Fos expression. Anat Rec (Hoboken). 293:1207–1213. 2010. View Article : Google Scholar : PubMed/NCBI
11	Zheng CH, Zhang J, Wu J and Zhang MM: The effect of transcutaneous electrical acupoint stimulation on pregnancy rates in women undergoing in vitro fertilization: A study protocol for a randomized controlled trial. Trials. 15:1622014. View Article : Google Scholar : PubMed/NCBI
12	Chapman CR, Schimek F, Gehrig JD, Gerlach R and Colpitts YH: Effects of nitrous oxide, transcutaneous electrical stimulation, and their combination on brain potentials elicited by painful stimulation. Anesthesiology. 58:250–256. 1983. View Article : Google Scholar : PubMed/NCBI
13	Hopwood V: Acupuncture in Physiotherapy: Key Concepts and Evidence Based-Practice. Allen H and Edwards R: (1st). Oxford UK: Butterworth-Heinemann. 2004. View Article : Google Scholar
14	Kondo T and Kawamoto M: Acupuncture and moxibustion for stress-related disorders. Biopsychosoc Med. 8:72014. View Article : Google Scholar : PubMed/NCBI
15	McQuade JL, Meng Z, Chen Z, Wei Q, Zhang Y, Bei W, Palmer JL and Cohen L: Utilization of and attitudes towards Traditional Chinese Medicine therapies in a Chinese cancer hospital, A survey of patients and physicians. Evid Based Complement Alternat Med. 2012:5045072012. View Article : Google Scholar : PubMed/NCBI
16	Little JP: Consitency of ASA grading. Anaesthesia. 50:658–659. 1995.PubMed/NCBI
17	Goldstraw P, Crowley J, Chansky K, Giroux DJ, Groome PA, Rami-Porta R, Postmus PE, Rusch V and Sobin L: International Association for the Study of Lung Cancer International Staging Committee; Participating Institutions: The IASLC Lung Cancer Staging Project. Proposals for the revision of the TNM stage groupings in the forthcoming (seventh) edition of the TNM Classification of malignant tumours. J Thorac Oncol. 2:706–714. 2007. View Article : Google Scholar : PubMed/NCBI
18	Alfara Fibla JJ: GómezS ebastián G, Farina Ríos C, Carvajal Carrasco A, Estrada Saló G and León González C: Lobectomy versus limited resection to treat non-small cell lung cancer in stage I: A study of 78 cases. Arch Bronconeumol. 39:217–220. 2003.PubMed/NCBI
19	Chen LL, Hsu SF, Wang MH, Chen CL, Lin YD and Lai JS: Use of acupressure to improve gastrointestinal motility in women after trans-abdominal hysterectomy. Am J Chin Med. 31:781–790. 2003. View Article : Google Scholar : PubMed/NCBI
20	Xu HY, Yang F, Zhu J, He ZP and Yan C: Effect of electroacupuncture at Hegu (LI 4) and Sanyinjiao (SP 6) on short-term adverse effects of drug-induced abortion. Zhongguo Zhen Jiu. 27:103–105. 2007.(In Chinese). PubMed/NCBI
21	Fu GQ, Zhou J, Tong QY, et al: Observation on the anti-stress effect of acupuncture-assisted anesthesia for pulmonary lobectomy patients. Zhen Ci Yan Jiu. 36:361–365. 2011.PubMed/NCBI
22	Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e452001. View Article : Google Scholar : PubMed/NCBI
23	Hunter JD: Effects of anaesthesia on the human immune system. Hosp Med. 60:658–663. 1999. View Article : Google Scholar : PubMed/NCBI
24	Engers R, Mueller M, Walter A, Collard JG, Willers R and Gabbert HE: Prognostic relevance of Tiam1 protein expression in prostate carcinomas. Br J Cancer. 95:1081–1086. 2006. View Article : Google Scholar : PubMed/NCBI
25	Chernyak GV and Sessler DI: Perioperative acupuncture and related techniques. Anesthesiology. 102:1031–1049. 2005. View Article : Google Scholar : PubMed/NCBI
26	Zhao L, Yang J, Wang HP and Liu RY: Imbalance in the Th17/Treg and cytokine environment in peripheral blood of patients with adenocarcinoma and squamous cell carcinoma. Med Oncol. 30:4612013. View Article : Google Scholar : PubMed/NCBI
27	Wang K, Wu H, Wang G, Li M, Zhang Z and Gu G: The effects of electroacupuncture on TH1/TH2 cytokine mRNA expression and mitogen-activated protein kinase signaling pathways in the splenic T cells of traumatized rats. Anesth Analg. 109:1666–1673. 2009. View Article : Google Scholar : PubMed/NCBI
28	Li G, Li S, Wang B and An L: The effect of electroacupuncture on postoperative immunoinflammatory response in patients undergoing supratentorial craniotomy. Exp Ther Med. 6:699–702. 2013.PubMed/NCBI
29	Chen C, Chen D, Zhang Y, Chen Z, Zhu W, Zhang B, Wang Z and Le H: Changes of CD4⁺CD25⁺FOXP3⁺ and CD8⁺CD28⁻ regulatory T cells in non-small cell lung cancer patients undergoing surgery. Int Immunopharmacol. 18:255–261. 2014. View Article : Google Scholar : PubMed/NCBI
30	Lv JQ, Feng RZ and Li N: P6 acupoint stimulation for prevention of postoperative nausea and vomiting in patients undergoing craniotomy, Study protocol for a randomized controlled trial. Trials. 14:1532013. View Article : Google Scholar : PubMed/NCBI
31	Hughes GS Jr, Lichstein PR, Whitlock D and Harker C: Response of plasma beta-endorphins to transcutaneous electrical nerve stimulation in healthy subjects. Phys Ther. 64:1062–1066. 1984.PubMed/NCBI
32	Ngai SP, Jones AY, Hui-Chan CW, Ko FW and Hui DS: Effect of 4 weeks of Acu-TENS on functional capacity and beta-endorphin level in subjects with chronic obstructive pulmonary disease, A randomized controlled trial. Respir Physiol Neurobiol. 173:29–36. 2010. View Article : Google Scholar : PubMed/NCBI