Increased 3β‑hydroxysteroid dehydrogenase 2 and 17α‑hydroxylase activities in a virilized adolescent female with adrenal adenoma: A case report

  • Authors:
    • Guoqing Yang
    • Jingtao Dou
    • Xiaolin Zhang
    • Weijun Gu
    • Zhaohui Lv
    • Jin Du
    • Jianming Ba
    • Yiming Mu
    • Juming Lu
  • View Affiliations

  • Published online on: December 14, 2015     https://doi.org/10.3892/etm.2015.2935
  • Pages: 530-534
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Abstract

In the present study, the case of a female patient with pseudo‑hermaphrodism caused by an androgen‑producing adrenocortical tumor is presented, and the possible mechanism is investigated. The expression of the luteinizing hormone/human chorionic gonadotrophin (LH/hCG) receptor in tumor tissues and normal adrenal tissues was analyzed using immunohistochemistry. Furthermore, the activities of 3β‑hydroxysteroid dehydrogenase 2 (HSD2), cytochrome P450 17α‑hydroxylase (CYP17) and 17β‑hydroxysteroid dehydrogenase 3 (HSD3) enzymes were measured using enzyme‑linked immunosorbent assay, and the expression levels of 3β‑HSD2, 17β‑HSD3, CYP17 and LH/hCG receptor mRNA were determined by quantitative polymerase chain reaction (qPCR). Immunohistochemical staining for the LH/hCG receptor was negative in the tumor tissue and positive in the normal adrenal tissue. The activities of 3β‑HSD2 and CYP17 in the tumor tissue were higher than those in the normal tissue (P<0.01), whereas the activity of 17β‑HSD3 was lower (P<0.01). The mRNA levels of 3β‑HSD2 and CYP17 were higher (P<0.01) and the levels of 17β‑HSD3 and LH/hCG receptor were lower (P<0.01) in the tumor tissue compared with those of the normal tissue. In conclusion, in the present study, a rare case of virilization by an androgen‑producing adrenocortical tumor is present. The results indicate that it may be associated with increased activities of 3β‑HSD2 and CYP17 but not with the expression of the LH/hCG receptor.
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February-2016
Volume 11 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yang G, Dou J, Zhang X, Gu W, Lv Z, Du J, Ba J, Mu Y and Lu J: Increased 3β‑hydroxysteroid dehydrogenase 2 and 17α‑hydroxylase activities in a virilized adolescent female with adrenal adenoma: A case report. Exp Ther Med 11: 530-534, 2016
APA
Yang, G., Dou, J., Zhang, X., Gu, W., Lv, Z., Du, J. ... Lu, J. (2016). Increased 3β‑hydroxysteroid dehydrogenase 2 and 17α‑hydroxylase activities in a virilized adolescent female with adrenal adenoma: A case report. Experimental and Therapeutic Medicine, 11, 530-534. https://doi.org/10.3892/etm.2015.2935
MLA
Yang, G., Dou, J., Zhang, X., Gu, W., Lv, Z., Du, J., Ba, J., Mu, Y., Lu, J."Increased 3β‑hydroxysteroid dehydrogenase 2 and 17α‑hydroxylase activities in a virilized adolescent female with adrenal adenoma: A case report". Experimental and Therapeutic Medicine 11.2 (2016): 530-534.
Chicago
Yang, G., Dou, J., Zhang, X., Gu, W., Lv, Z., Du, J., Ba, J., Mu, Y., Lu, J."Increased 3β‑hydroxysteroid dehydrogenase 2 and 17α‑hydroxylase activities in a virilized adolescent female with adrenal adenoma: A case report". Experimental and Therapeutic Medicine 11, no. 2 (2016): 530-534. https://doi.org/10.3892/etm.2015.2935