Protective effect of microRNA-138 against cerebral ischemia/reperfusion injury in rats

  • Authors:
    • Xiang‑Jun Tang
    • Ming‑Huan Yang
    • Gang Cao
    • Jun‑Ti Lu
    • Jie Luo
    • Long‑Jun Dai
    • Kuan‑Ming Huang
    • Li Zhang
  • View Affiliations

  • Published online on: January 22, 2016     https://doi.org/10.3892/etm.2016.3021
  • Pages: 1045-1050
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Abstract

MicroRNAs (miRs) serve a regulatory function in oxidative radical-mediated inflammation and apoptosis during ischemia/reperfusion (IR) injury. Lipocalin 2 (Lcn‑2), a target protein of miR‑138, is widely involved in the systemic response to IR injury. The aim of the present study was to investigate the association between miR‑138 and Lcn‑2 in a rat model of cerebral ischemia/reperfusion (CIR) injury and to verify the interaction between miR‑138 and Lcn‑2 in a PC12 cell model of hypoxia/reoxygenation injury. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to detect the mRNA and protein expression levels of miR‑138 and Lcn‑2. Cell proliferation was determined by MTT assay. The results suggested that the expression of miR‑138 was inversely correlated with the expression of Lcn-2 in the CIR rat model and the PC12 cells subjected to hypoxia and reoxygenation. The expression of Lcn‑2 was inhibited by miR‑138 mimics and enhanced by miR‑138 inhibitors, thereby indicating that miR‑138 functions as a negative regulator for Lcn‑2 expression. This study provides an experimental basis for the further study of miR-138-based therapy for CIR injury.
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March-2016
Volume 11 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Tang XJ, Yang MH, Cao G, Lu JT, Luo J, Dai LJ, Huang KM and Zhang L: Protective effect of microRNA-138 against cerebral ischemia/reperfusion injury in rats. Exp Ther Med 11: 1045-1050, 2016
APA
Tang, X., Yang, M., Cao, G., Lu, J., Luo, J., Dai, L. ... Zhang, L. (2016). Protective effect of microRNA-138 against cerebral ischemia/reperfusion injury in rats. Experimental and Therapeutic Medicine, 11, 1045-1050. https://doi.org/10.3892/etm.2016.3021
MLA
Tang, X., Yang, M., Cao, G., Lu, J., Luo, J., Dai, L., Huang, K., Zhang, L."Protective effect of microRNA-138 against cerebral ischemia/reperfusion injury in rats". Experimental and Therapeutic Medicine 11.3 (2016): 1045-1050.
Chicago
Tang, X., Yang, M., Cao, G., Lu, J., Luo, J., Dai, L., Huang, K., Zhang, L."Protective effect of microRNA-138 against cerebral ischemia/reperfusion injury in rats". Experimental and Therapeutic Medicine 11, no. 3 (2016): 1045-1050. https://doi.org/10.3892/etm.2016.3021