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Article

Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression

  • Authors:
    • Liping Yang
    • Xueyan Sun
    • Yongli Zhan
    • Huijie Liu
    • Yumin Wen
    • Huimin Mao
    • Xi Dong
    • Ping Li
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Guang'Anmen Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100053, P.R. China, Beijing University of Chinese Medicine, Chaoyang, Liaoning 100029, P.R. China, Department of Pharmacology, Institute of Clinical Medical Sciences, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
  • Pages: 1410-1416
    |
    Published online on: January 28, 2016
       https://doi.org/10.3892/etm.2016.3027
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Abstract

The aim of the present study was to investigate the effect of Yi Qi Qing Re Gao-containing serum (YQ‑S) on rat mesangial cell (MC) proliferation and to investigate the underlying mechanism. MCs were divided into the control, lipopolysaccharide (LPS)-stimulated, YQ‑S and fosinopril-containing serum (For‑S) groups, and cultured for 48 h. An MTT assay was used to evaluate the proliferation of MCs. In addition, reverse transcription‑quantitative polymerase chain reaction and western blot analysis were conducted to detect the expression levels of Wnt4, β‑catenin and transforming growth factor (TGF)‑β1 in MCs. The results indicated that YQ‑S inhibited LPS‑induced MC proliferation. The Wnt4 and TGF‑β1 mRNA expression levels were reduced in the YQ‑S group (P<0.01 or P<0.05). Furthermore, the Wnt4, β‑catenin and TGF‑β1 protein expression levels were suppressed in the YQ‑S group (P<0.01 or P<0.05). Therefore, YQ‑S appears to inhibit MC proliferation, and its mechanism may involve the inhibition of the Wnt signaling pathway and downregulation of TGF-β1 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Sun X, Zhan Y, Liu H, Wen Y, Mao H, Dong X and Li P: Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression. Exp Ther Med 11: 1410-1416, 2016.
APA
Yang, L., Sun, X., Zhan, Y., Liu, H., Wen, Y., Mao, H. ... Li, P. (2016). Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression. Experimental and Therapeutic Medicine, 11, 1410-1416. https://doi.org/10.3892/etm.2016.3027
MLA
Yang, L., Sun, X., Zhan, Y., Liu, H., Wen, Y., Mao, H., Dong, X., Li, P."Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression". Experimental and Therapeutic Medicine 11.4 (2016): 1410-1416.
Chicago
Yang, L., Sun, X., Zhan, Y., Liu, H., Wen, Y., Mao, H., Dong, X., Li, P."Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1410-1416. https://doi.org/10.3892/etm.2016.3027
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Sun X, Zhan Y, Liu H, Wen Y, Mao H, Dong X and Li P: Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression. Exp Ther Med 11: 1410-1416, 2016.
APA
Yang, L., Sun, X., Zhan, Y., Liu, H., Wen, Y., Mao, H. ... Li, P. (2016). Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression. Experimental and Therapeutic Medicine, 11, 1410-1416. https://doi.org/10.3892/etm.2016.3027
MLA
Yang, L., Sun, X., Zhan, Y., Liu, H., Wen, Y., Mao, H., Dong, X., Li, P."Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression". Experimental and Therapeutic Medicine 11.4 (2016): 1410-1416.
Chicago
Yang, L., Sun, X., Zhan, Y., Liu, H., Wen, Y., Mao, H., Dong, X., Li, P."Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1410-1416. https://doi.org/10.3892/etm.2016.3027
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