Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression

Yang,Liping; Sun,Xueyan; Zhan,Yongli; Liu,Huijie; Wen,Yumin; Mao,Huimin; Dong,Xi; Li,Ping

doi:10.3892/etm.2016.3027

Yi Qi Qing Re Gao-containing serum inhibits lipopolysaccharide-induced rat mesangial cell proliferation by suppressing the Wnt pathway and TGF-β1 expression

Authors:
- Liping Yang
- Xueyan Sun
- Yongli Zhan
- Huijie Liu
- Yumin Wen
- Huimin Mao
- Xi Dong
- Ping Li
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Affiliations: Department of Nephrology, Guang'Anmen Hospital of China Academy of Traditional Chinese Medicine Sciences, Beijing 100053, P.R. China, Beijing University of Chinese Medicine, Chaoyang, Liaoning 100029, P.R. China, Department of Pharmacology, Institute of Clinical Medical Sciences, China‑Japan Friendship Hospital, Beijing 100029, P.R. China
Published online on: January 28, 2016 https://doi.org/10.3892/etm.2016.3027
Pages: 1410-1416

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Abstract

The aim of the present study was to investigate the effect of Yi Qi Qing Re Gao-containing serum (YQ‑S) on rat mesangial cell (MC) proliferation and to investigate the underlying mechanism. MCs were divided into the control, lipopolysaccharide (LPS)-stimulated, YQ‑S and fosinopril-containing serum (For‑S) groups, and cultured for 48 h. An MTT assay was used to evaluate the proliferation of MCs. In addition, reverse transcription‑quantitative polymerase chain reaction and western blot analysis were conducted to detect the expression levels of Wnt4, β‑catenin and transforming growth factor (TGF)‑β1 in MCs. The results indicated that YQ‑S inhibited LPS‑induced MC proliferation. The Wnt4 and TGF‑β1 mRNA expression levels were reduced in the YQ‑S group (P<0.01 or P<0.05). Furthermore, the Wnt4, β‑catenin and TGF‑β1 protein expression levels were suppressed in the YQ‑S group (P<0.01 or P<0.05). Therefore, YQ‑S appears to inhibit MC proliferation, and its mechanism may involve the inhibition of the Wnt signaling pathway and downregulation of TGF-β1 expression.

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