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Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway

  • Authors:
    • Yan Sun
    • Caimei Xiu
    • Wei Liu
    • Yuan Tao
    • Jianrong Wang
    • Yi Qu
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Jinan Second People's Hospital, Jinan, Shandong 250001, P.R. China, Department of Ophthalmology, Yantai Yuhuangding Hospital Affiliated to Medical College of Qingdao University, Yantai, Shandong 264000, P.R. China, Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1253-1258
    |
    Published online on: January 29, 2016
       https://doi.org/10.3892/etm.2016.3033
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Abstract

The present study aimed to investigate whether grape seed proanthocyanidin extract (GSPE) has a protective effect on diabetic retinal function. A total of 30 Wistar rats were randomly divided into three equal groups, including the control, diabetic and GSPE‑treated diabetic groups. Retinal tissue was harvested and subsequently stained with hematoxylin and eosin. Superoxide dismutase (SOD), glutathione peroxidase (GSH‑Px) and methane dicarboxylic aldehyde (MDA) levels were evaluated using respective assay kits; whereas nuclear erythroid 2‑related factor 2 (Nrf2) and heme oxygenase (HO)‑1 expression levels were assessed by immunohistochemical and western blot analysis. Cell apoptosis in the retina was determined using the terminal deoxynucleotidyl transferase‑mediated dUTP nick‑end labeling method. The results showed that the structure of the retina was damaged in diabetic rats, as compared with the control rats. Notably, the structure of the retina improved in the GSPE‑treated diabetic group, as compared with the diabetic group. SOD and GSH‑Px activities were significantly increased in the retina of rats in the GSPE‑treated diabetic group, as compared with the diabetic group (P=0.011 and P=0.001, respectively). Furthermore, a significant reduction in MDA was detected (P=0.013) and the expression levels of Nrf2 and HO‑1 in the bladders of rats in the GSPE‑treated diabetic group were significantly increased, as compared with the diabetic group (P=0.038 and P=0.043, respectively). Apoptosis of retinal cells was significantly increased in the diabetic group, as compared with the control group (P<0.001); a significant reduction was also detected in the GSPE‑treated diabetic group, as compared with the diabetic group (P=0.014). These results demonstrate that GSPE administration may protect the retina against hyperglycemic damage, possibly by ameliorating oxidative stress‑mediated injury via the activation of the Nrf2 pathway.
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1 

Ruta LM, Magliano DJ, Lemesurier R, Taylor HR, Zimmet PZ and Shaw JE: Prevalence of diabetic retinopathy in Type 2 diabetes in developing and developed countries. Diabet Med. 30:387–398. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Man RE, Sasongko MB, Wang JJ, MacIsaac R, Wong TY, Sabanayagam C and Lamoureux EL: The Association of Estimated Glomerular Filtration Rate With Diabetic Retinopathy and Macular Edema. Invest Ophthalmol Vis Sci. 56:4810–4816. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Malek M, Khamseh ME, Aghili R, Emami Z, Najafi L and Baradaran HR: Medical management of diabetic retinopathy: An overview. Arch Iran Med. 15:635–640. 2012.PubMed/NCBI

4 

Barber AJ, Gardner TW and Abcouwer SF: The significance of vascular and neural apoptosis to the pathology of diabetic retinopathy. Invest Ophthalmol Vis Sci. 52:1156–1163. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Hu WK, Liu R, Pei H and Li B: Endoplasmic reticulum stress-related factors protect against diabetic retinopathy. Exp Diabetes Res. 2012:5079862012. View Article : Google Scholar : PubMed/NCBI

6 

Williams M, Hogg RE and Chakravarthy U: Antioxidants and diabetic retinopathy. Curr Diab Rep. 13:481–487. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Ferreira D and Li XC: Oligomeric proanthocyanidins: Naturally occurring O-heterocycles. Nat Prod Rep. 17:193–212. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Houde V, Grenier D and Chandad F: Protective effects of grape seed proanthocyanidins against oxidative stress induced by lipopolysaccharides of periodontopathogens. J Periodontol. 77:1371–1379. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Li M, Ma YB, Gao HQ, Li BY, Cheng M, Xu L, Li XL and Li XH: A novel approach of proteomics to study the mechanism of action of grape seed proanthocyanidin extracts on diabetic retinopathy in rats. Chin Med J (Engl). 121:2544–2552. 2008.PubMed/NCBI

10 

Kaspar JW, Niture SK and Jaiswal AK: Nrf2:INrf2 (Keap1) signaling in oxidative stress. Free Radic Biol Med. 47:1304–1309. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Zhong Q, Mishra M and Kowluru RA: Transcription factor Nrf2-mediated antioxidant defense system in the development of diabetic retinopathy. Invest Ophthalmol Vis Sci. 54:3941–3948. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Xu Z, Wei Y, Gong J, Cho H, Park JK, Sung ER, Huang H, Wu L, Eberhart C, Handa JT, et al: NRF2 plays a protective role in diabetic retinopathy in mice. Diabetologia. 57:204–213. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Tan SM and De Haan JB: Combating oxidative stress in diabetic complications with Nrf2 activators: How much is too much? Redox Rep. 19:107–117. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Wang S, Park JK and Duh EJ: Novel targets against retinal angiogenesis in diabetic retinopathy. Curr Diab Rep. 12:355–363. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Scapagnini G, Vasto S, Abraham NG, Caruso C, Zella D and Fabio G: Modulation of Nrf2/ARE pathway by food polyphenols: A nutritional neuroprotective strategy for cognitive and neurodegenerative disorders. Mol Neurobiol. 44:192–201. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Jeong IK and King GL: New perspectives on diabetic vascular complications: The loss of endogenous protective factors induced by hyperglycemia. Diabetes Metab J. 35:8–11. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Sun JK, Keenan HA, Cavallerano JD, Asztalos BF, Schaefer EJ, Sell DR, Strauch CM, Monnier VM, Doria A, Aiello LP and King GL: Protection from retinopathy and other complications in patients with type 1 diabetes of extreme duration: The joslin 50-year medalist study. Diabetes Care. 34:968–974. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Kojima K, Maki K, Tofani I, Kamitani Y and Kimura M: Effects of grape seed proanthocyanidins extract on rat mandibular condyle. J Musculoskelet Neuronal Interact. 4:301–307. 2004.PubMed/NCBI

19 

Mansouri E, Panahi M, Ghaffari MA and Ghorbani A: Effects of grape seed proanthocyanidin extract on oxidative stress induced by diabetes in rat kidney. Iran Biomed J. 15:100–106. 2011.PubMed/NCBI

20 

Okudan N, Barışkaner H, Gökbel H, Sahin AS, Belviranlı M and Baysal H: The effect of supplementation of grape seed proanthocyanidin extract on vascular dysfunction in experimental diabetes. J Med Food. 14:1298–1302. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Ding Y, Dai X, Jiang Y, Zhang Z, Bao L, Li Y, Zhang F, Ma X, Cai X, Jing L, et al: Grape seed proanthocyanidin extracts alleviate oxidative stress and ER stress in skeletal muscle of low-dose streptozotocin- and high-carbohydrate/high-fat diet-induced diabetic rats. Mol Nutr Food Res. 57:365–369. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Cui XP, Li BY, Gao HQ, Wei N, Wang WL and Lu M: Effects of grape seed proanthocyanidin extracts on peripheral nerves in streptozocin-induced diabetic rats. J Nutr Sci Vitaminol (Tokyo). 54:321–328. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Yamagishi S and Matsui T: Advanced glycation end products (AGEs), oxidative stress and diabetic retinopathy. Curr Pharm Biotechnol. 12:362–368. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Bagchi D, Sen CK, Ray SD, Das DK, Bagchi M, Preuss HG and Vinson JA: Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract. Mutat Res 523–524. 87–97. 2003. View Article : Google Scholar

25 

Zou X, Feng Z, Li Y, Wang Y, Wertz K, Weber P, Weber P, Fu Y and Liu J: Stimulation of GSH synthesis to prevent oxidative stress-induced apoptosis by hydroxytyrosol in human retinal pigment epithelial cells: Activation of Nrf2 and JNK-p62/SQSTM1 pathways. J Nutr Biochem. 23:994–1006. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Pehar M, Vargas MR, Robinson KM, Cassina P, Díaz-Amarilla PJ, Hagen TM, Radi R, Barbeito L and Beckman JS: Mitochondrial superoxide production and nuclear factor erythroid 2-related factor 2 activation in p75 neurotrophin receptor-induced motor neuron apoptosis. J Neurosci. 27:7777–7785. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Fan J, Xu G, Jiang T and Qin Y: Pharmacologic induction of heme oxygenase-1 plays a protective role in diabetic retinopathy in rats. Invest Ophthalmol Vis Sci. 53:6541–6556. 2012. View Article : Google Scholar : PubMed/NCBI

28 

He M, Pan H, Chang RC, So KF, Brecha NC and Pu M: Activation of the Nrf2/HO-1 antioxidant pathway contributes to the protective effects of Lycium barbarum polysaccharides in the rodent retina after ischemia reperfusion induced damage. PloS One. 9:e848002014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun Y, Xiu C, Liu W, Tao Y, Wang J and Qu Y: Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway. Exp Ther Med 11: 1253-1258, 2016.
APA
Sun, Y., Xiu, C., Liu, W., Tao, Y., Wang, J., & Qu, Y. (2016). Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway. Experimental and Therapeutic Medicine, 11, 1253-1258. https://doi.org/10.3892/etm.2016.3033
MLA
Sun, Y., Xiu, C., Liu, W., Tao, Y., Wang, J., Qu, Y."Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway". Experimental and Therapeutic Medicine 11.4 (2016): 1253-1258.
Chicago
Sun, Y., Xiu, C., Liu, W., Tao, Y., Wang, J., Qu, Y."Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1253-1258. https://doi.org/10.3892/etm.2016.3033
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Y, Xiu C, Liu W, Tao Y, Wang J and Qu Y: Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway. Exp Ther Med 11: 1253-1258, 2016.
APA
Sun, Y., Xiu, C., Liu, W., Tao, Y., Wang, J., & Qu, Y. (2016). Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway. Experimental and Therapeutic Medicine, 11, 1253-1258. https://doi.org/10.3892/etm.2016.3033
MLA
Sun, Y., Xiu, C., Liu, W., Tao, Y., Wang, J., Qu, Y."Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway". Experimental and Therapeutic Medicine 11.4 (2016): 1253-1258.
Chicago
Sun, Y., Xiu, C., Liu, W., Tao, Y., Wang, J., Qu, Y."Grape seed proanthocyanidin extract protects the retina against early diabetic injury by activating the Nrf2 pathway". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1253-1258. https://doi.org/10.3892/etm.2016.3033
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