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Article

miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation

  • Authors:
    • Nan Cheng
    • Guang‑Hui Wang
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, Huaihe Hospital, Henan University, Kaifeng, Henan 475000, P.R. China
  • Pages: 1469-1474
    |
    Published online on: February 1, 2016
       https://doi.org/10.3892/etm.2016.3043
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Abstract

MicroRNAs (miRs) are a class of short and non-coding RNA molecules, which function as either oncogenes or tumor suppressors in the development of various human cancers, including nasopharyngeal carcinoma (NPC). The aim of the present study was to investigate the expression of miR-133b in NPC tissue samples, as compared with adjacent normal tissues, and to examine its roles and underlying mechanisms. Analysis using reverse transcription‑quantitative polymerase chain reaction demonstrated that miR‑133b was downregulated in NPC tissue samples, as compared with adjacent tissues. In vitro experiments using NPC cell lines transfected with miR‑133b mimics or antisense oligonucleotides further demonstrated that the overexpression of miR‑133b mimics impaired, whereas knockdown of its expression promoted, the proliferation of NPC cells. Sphingosine‑1‑phosphate receptor 1 (S1PR1) was predicted to be a target of miR‑133b. Luciferase reporter assays showed that miR‑133b inhibited the protein expression of S1PR1 by targeting its 3'‑untranslated region. Furthermore, western blot analysis demonstrated that miR‑133B altered the regulation of the signal transducer and activator of transcription-3 (STAT3) signaling pathway and the expression of downstream proteins in NPC cells. Therefore, the results of the present study suggested that a previously unknown miR-133b/S1PR1 molecular network may regulate NPC progression.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng N and Wang GH: miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation. Exp Ther Med 11: 1469-1474, 2016.
APA
Cheng, N., & Wang, G. (2016). miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation. Experimental and Therapeutic Medicine, 11, 1469-1474. https://doi.org/10.3892/etm.2016.3043
MLA
Cheng, N., Wang, G."miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation". Experimental and Therapeutic Medicine 11.4 (2016): 1469-1474.
Chicago
Cheng, N., Wang, G."miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1469-1474. https://doi.org/10.3892/etm.2016.3043
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng N and Wang GH: miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation. Exp Ther Med 11: 1469-1474, 2016.
APA
Cheng, N., & Wang, G. (2016). miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation. Experimental and Therapeutic Medicine, 11, 1469-1474. https://doi.org/10.3892/etm.2016.3043
MLA
Cheng, N., Wang, G."miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation". Experimental and Therapeutic Medicine 11.4 (2016): 1469-1474.
Chicago
Cheng, N., Wang, G."miR-133b, a microRNA targeting S1PR1, suppresses nasopharyngeal carcinoma cell proliferation". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1469-1474. https://doi.org/10.3892/etm.2016.3043
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