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Article

Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone

  • Authors:
    • Xiangyun Liu
    • Yi Cheng
    • Qi Pan
    • Wenjuan Hu
    • Li Xu
    • Xiang Meng
    • Jianhui Wu
    • Chenjing Xie
    • Han Yan
    • Zuyue Sun
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Exercise and Health Sciences (Shanghai University of Sport), Ministry of Education, Shanghai 200438, P.R. China, Laboratory Testing Division, WuXi AppTec (Shanghai) Co., Ltd., Shanghai 200131, P.R. China, Department of Pharmacology and Toxicology, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, P.R. China, Department of Pharmacy, Shanghai Children's Hospital, Shanghai Jiatong University, Shanghai 200040, P.R. China
  • Pages: 1361-1366
    |
    Published online on: February 1, 2016
       https://doi.org/10.3892/etm.2016.3044
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Abstract

The aim of the present study was to investigate the changes in mitotic reorientation and relative differential gene expression in rat prostate epithelial cells following long‑term exposure to testosterone propionate (TP). Sprague‑Dawley rats were randomly divided into two groups as follows: TP group, which received 3.7 mg/kg/day TP for 30 days (n=10); and control group, in which rats were injected with olive oil (n=10). Microscopic analysis of the prostate tissue was performed by immunohistochemical analysis and hematoxylin and eosin staining. Differential gene expression analysis was performed via gene microarray, and a total of five genes (Dkk3, Ran, Fas, Tgm4 and Wnt2) were selected and their expression levels were verified using reverse transcription‑polymerase chain reaction. For rats treated with TP, mitosis was significantly reoriented, becoming parallel to the basement membrane. By contrast, in the control group cells mitotic orientation remained perpendicular to the basement membrane. Genes such as Ran and Tgm4 in the androgen receptor (AR) signaling pathway and Wnt2 in the Wnt signaling pathway, were upregulated following treatment with TP. Conversely, the Dkk3 and Fas genes were downregulated following treatment with TP. In conclusion, mitotic orientation of prostate epithelial cells was altered following long‑term administration of TP. Wnt and AR signaling pathways influenced cell proliferation and may have participated in the mitotic orientation change.
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1 

Berry SJ, Coffey DS, Walsh PC and Ewing LL: The development of human benign prostatic hyperplasia with age. J Urol. 132:474–479. 1984.PubMed/NCBI

2 

Liu XY, Li DM, Zhang XF, Wu JH and Sun ZY: Mitosis orientation in prostate epithelial cells changed by endocrine effect. Acta Pharmacol Sin. 29:226–229. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Gao W: Androgen receptor as a therapeutic target. Adv Drug Deliv Rev. 62:1277–1284. 2010. View Article : Google Scholar : PubMed/NCBI

4 

Chatterjee B: The role of the androgen receptor in the development of prostatic hyperplasia and prostate cancer. Mol Cell Biochem. 253:89–101. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Beltrami AP, Cesselli D and Beltrami CA: At the stem of youth and health. Pharmacol Ther. 129:3–20. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Isaacs JT and Coffey DS: Etiology and disease process of benign prostatic hyperplasia. Prostate Suppl. 2:33–50. 1989. View Article : Google Scholar : PubMed/NCBI

7 

Mansour FH and Pestov DG: Separation of long RNA by agarose-formaldehyde gel electrophoresis. Anal Biochem. 441:18–20. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Vignozzi L, Gacci M, Cellai I, Santi R, Corona G, Morelli A, Rastrelli G, Comeglio P, Sebastanelli A, Maneschi E, et al: Fat boosts, while androgen receptor activation counteracts, BPH-associated prostate inflammation. Prostate. 73:789–800. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Xiang-Yun L, Ying-Wen X, Chen-Jing X, Jiu-Jiu W, Qi P, Bo G and Zu-Yue S: Possible mechanism of benign prostatic hyperplasia induced by androgen-estrogen ratios in castrated rats. Indian J Pharmacol. 42:312–317. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Hartman J, Ström A and Gustafsson JÅ: Current concepts and significance of estrogen receptor β in prostate cancer. Steroids. 77:1262–1266. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Logan CY and Nusse R: The Wnt signaling pathway in development and disease. Annu Rev Cell Dev Biol. 20:781–810. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Polakis P: Wnt signaling and cancer. Genes Dev. 14:1837–1851. 2000.PubMed/NCBI

13 

Ye X, Zerlanko B, Kennedy A, Banumathy G, Zhang R and Adams PD: Downregulation of Wnt signaling is a trigger for formation of facultative heterochromatin and onset of cell senescence in primary human cells. Mol Cell. 27:183–196. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Levasseur R, Lacombe D and de Vernejoul MC: LRP5 mutations in osteoporosis-pseudoglioma syndrome and high-bone-mass disorders. Joint Bone Spine. 72:207–214. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Davidson G, Mao B, del Barco Barrantes I and Niehrs C: Kremen proteins interact with Dickkopf1 to regulate anteroposterior CNS patterning. Development. 129:5587–5596. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Abarzua F, Sakaguchi M, Takaishi M, Nasu Y, Kurose K, Ebara S, Miyazaki M, Namba M, Kumon H and Huh NH: Adenovirus-mediated overexpression of REIC/Dkk-3 selectively induces apoptosis in human prostate cancer cells through activation of c-Jun-NH2-kinase. Cancer Res. 65:9617–9622. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Hsieh SY, Hsieh PS, Chiu CT and Chen WY: Dickkopf-3/REIC functions as a suppressor gene of tumor growth. Oncogene. 23:9183–9189. 2004.PubMed/NCBI

18 

Kawano Y, Kitaoka M, Hamada Y, Walker MM, Waxman J and Kypta RM: Regulation of prostate cell growth and morphogenesis by Dickkopf-3. Oncogene. 25:6528–6537. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Li H, Wu C, Gu X and Xiong C: A novel application of cell-free seminal mRNA: Non-invasive identification of the presence of germ cells or complete obstruction in men with azoospermia. Hum Reprod. 27:991–997. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Xu B, Harihan A, Rakshit S, Dressler GR and Wellik DM: The role of Pax2 in mouse prostate development. Prostate. 72:217–224. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Thielen JL, Volzing KG, Collier LS, Green LE, Largaespada DA and Marker PC: Markers of prostate region-specific epithelial identity define anatomical locations in the mouse prostate that are molecularly similar to human prostate cancers. Differentiation. 75:49–61. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Ran L, He W, Zhu X, Zhou Q and Gou X: Comparison of fluid absorption between transurethral enucleation and transurethral resection for benign prostate hyperplasia. Urol Int. 91:26–30. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Newton CJ, Ran G, Xie YX, Bilko D, Burgoyne CH, Adams I, Abidia A, McCollum PT and Atkin SL: Statin-induced apoptosis of vascular endothelial cells is blocked by dexamethasone. J Endocrinol. 174:7–16. 2002. View Article : Google Scholar : PubMed/NCBI

24 

Niehrs C and Acebron SP: Mitotic and mitogenic Wnt signalling. EMBO J. 31:2705–2713. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Nandan MO, Chanchevalap S, Dalton WB and Yang VW: Krüppel-like factor 5 promotes mitosis by activating the cyclin B1/Cdc2 complex during oncogenic Ras-mediated transformation. FEBS Lett. 579:4757–4762. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Antonarakis ES, Lu C, Luber B, Wang H, Chen Y, Nakazawa M, Nadal R, Paller CJ, Denmeade SR, Carducci MA, et al: Androgen receptor splice variant 7 and efficacy of taxane chemotherapy in patients with metastatic castration-resistant prostate cancer. JAMA Oncol. 1:582–591. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Mukhopadhyay NK, Cinar B, Mukhopadhyay L, Lutchman M, Ferdinand AS, Kim J, Chung LW, Adam RM, Ray SK, Leiter AB, et al: The zinc finger protein ras-responsive element binding protein-1 is a coregulator of the androgen receptor: Implications for the role of the Ras pathway in enhancing androgenic signaling in prostate cancer. Mol Endocrinol. 21:2056–2070. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Choi HJ and Zhu BT: Critical role of cyclin B1/Cdc2 up-regulation in the induction of mitotic prometaphase arrest in human breast cancer cells treated with 2-methoxyestradiol. Biochim Biophys Acta. 1823:1306–1315. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu X, Cheng Y, Pan Q, Hu W, Xu L, Meng X, Wu J, Xie C, Yan H, Sun Z, Sun Z, et al: Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone. Exp Ther Med 11: 1361-1366, 2016.
APA
Liu, X., Cheng, Y., Pan, Q., Hu, W., Xu, L., Meng, X. ... Sun, Z. (2016). Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone. Experimental and Therapeutic Medicine, 11, 1361-1366. https://doi.org/10.3892/etm.2016.3044
MLA
Liu, X., Cheng, Y., Pan, Q., Hu, W., Xu, L., Meng, X., Wu, J., Xie, C., Yan, H., Sun, Z."Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone". Experimental and Therapeutic Medicine 11.4 (2016): 1361-1366.
Chicago
Liu, X., Cheng, Y., Pan, Q., Hu, W., Xu, L., Meng, X., Wu, J., Xie, C., Yan, H., Sun, Z."Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1361-1366. https://doi.org/10.3892/etm.2016.3044
Copy and paste a formatted citation
x
Spandidos Publications style
Liu X, Cheng Y, Pan Q, Hu W, Xu L, Meng X, Wu J, Xie C, Yan H, Sun Z, Sun Z, et al: Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone. Exp Ther Med 11: 1361-1366, 2016.
APA
Liu, X., Cheng, Y., Pan, Q., Hu, W., Xu, L., Meng, X. ... Sun, Z. (2016). Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone. Experimental and Therapeutic Medicine, 11, 1361-1366. https://doi.org/10.3892/etm.2016.3044
MLA
Liu, X., Cheng, Y., Pan, Q., Hu, W., Xu, L., Meng, X., Wu, J., Xie, C., Yan, H., Sun, Z."Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone". Experimental and Therapeutic Medicine 11.4 (2016): 1361-1366.
Chicago
Liu, X., Cheng, Y., Pan, Q., Hu, W., Xu, L., Meng, X., Wu, J., Xie, C., Yan, H., Sun, Z."Changes in mitotic reorientation and Wnt/AR signaling in rat prostate epithelial cells exposed to subchronic testosterone". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1361-1366. https://doi.org/10.3892/etm.2016.3044
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