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Article

Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway

  • Authors:
    • Meng‑Hua Dong
    • Qian Zhang
    • Yuan‑Yuan Wang
    • Bai‑Sui Zhou
    • Yu‑Fei Sun
    • Qiang Fu
  • View Affiliations / Copyright

    Affiliations: College of Basic Medicine, Binzhou Medical University, Yantai, Shandong 264003, P.R. China, Department of Oncology, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China, Department of Bone and Joint Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, Shandong 264003, P.R. China
  • Pages: 1475-1480
    |
    Published online on: February 9, 2016
       https://doi.org/10.3892/etm.2016.3061
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Abstract

Euphorbia fischeriana Steud, a traditional Chinese medicine, has been shown to inhibit the growth of various cancers by the induction of apoptosis and cell cycle arrest. The purpose of the present study was to investigate the association between the phosphoinositide‑3‑kinase (PI3K)/protein kinase B (Akt) signaling pathway and the inhibitory effect of Euphorbia fischeriana Steud on the growth and metastasis of melanoma B16 cells in vitro, and the underlying mechanisms. MTT assay results indicated that Euphorbia fischeriana Steud inhibited the growth of B16 cells in a time- and dose-dependent manner. Flow cytometric analysis revealed that Euphorbia fischeriana Steud markedly induced apoptosis of the B16 cells, with arrest at the G0/G1 phase of the cell cycle. In addition, in a Transwell assay Euphorbia fischeriana Steud significantly suppressed the migration of B16 cells. Western blot analysis revealed that the expression levels of phosphatase and tensin homolog (PTEN) were upregulated, and the phosphorylation of Akt was downregulated, which resulted in inhibition of the PI3K/Akt signaling pathway and the eventual suppression of its downstream targets, such as matrix metalloproteinase‑2 mRNA, in B16 cells. The results demonstrated that Euphorbia fischeriana Steud inhibited the growth and migration of B16 cells, possibly via modulation of the PI3K/Akt signaling pathway and upregulation of PTEN expression levels, in addition to downregulation of p-Akt expression. The aforementioned findings suggest that Euphorbia fischeriana Steud may have broad therapeutic applications in the treatment of malignant melanoma.
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Copy and paste a formatted citation
Spandidos Publications style
Dong MH, Zhang Q, Wang YY, Zhou BS, Sun YF and Fu Q: Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway. Exp Ther Med 11: 1475-1480, 2016.
APA
Dong, M., Zhang, Q., Wang, Y., Zhou, B., Sun, Y., & Fu, Q. (2016). Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway. Experimental and Therapeutic Medicine, 11, 1475-1480. https://doi.org/10.3892/etm.2016.3061
MLA
Dong, M., Zhang, Q., Wang, Y., Zhou, B., Sun, Y., Fu, Q."Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway". Experimental and Therapeutic Medicine 11.4 (2016): 1475-1480.
Chicago
Dong, M., Zhang, Q., Wang, Y., Zhou, B., Sun, Y., Fu, Q."Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1475-1480. https://doi.org/10.3892/etm.2016.3061
Copy and paste a formatted citation
x
Spandidos Publications style
Dong MH, Zhang Q, Wang YY, Zhou BS, Sun YF and Fu Q: Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway. Exp Ther Med 11: 1475-1480, 2016.
APA
Dong, M., Zhang, Q., Wang, Y., Zhou, B., Sun, Y., & Fu, Q. (2016). Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway. Experimental and Therapeutic Medicine, 11, 1475-1480. https://doi.org/10.3892/etm.2016.3061
MLA
Dong, M., Zhang, Q., Wang, Y., Zhou, B., Sun, Y., Fu, Q."Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway". Experimental and Therapeutic Medicine 11.4 (2016): 1475-1480.
Chicago
Dong, M., Zhang, Q., Wang, Y., Zhou, B., Sun, Y., Fu, Q."Euphorbia fischeriana Steud inhibits malignant melanoma via modulation of the phosphoinositide-3-kinase/Akt signaling pathway". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1475-1480. https://doi.org/10.3892/etm.2016.3061
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