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Article

Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model

  • Authors:
    • Yong Liu
    • Tao Yu
    • Xue‑Xiao Ma
    • Hong‑Fei Xiang
    • You‑Gu Hu
    • Bo‑Hua Chen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
  • Pages: 1399-1404
    |
    Published online on: February 9, 2016
       https://doi.org/10.3892/etm.2016.3063
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Abstract

The present study examined the effects of transforming growth factor (TGF)-β3, connective tissue growth factor (CTGF) and tissue inhibitor of metalloproteinase 1 (TIMP1) gene transduction, using a lentiviral vector, on rabbit intervertebral disc degeneration in vivo, with the intention of investigating their potential use in gene therapy. A model of lumbar intervertebral disc degeneration was created by needle puncture into the annulus fibrosus of 15 New Zealand white rabbits. Empty lentivirus or recombinant lentiviral plasmid lenti‑TGFβ3‑P2A‑CTGF‑T2A‑TIMP1 was injected into degenerative lumbar intervertebral discs (representing the control and experimental groups, respectively), whilst untreated degenerative lumbar intervertebral discs served as the puncture group. After 16 and 20 weeks, magnetic resonance imaging (MRI) was conducted and the changes in intensity on micrographs of degenerative intervertebral discs were measured. The mRNA levels of aggrecan and type II collagen in nucleus pulposus tissue were determined by reverse transcription‑polymerase chain reaction, and protein expression levels of type II collagen and aggrecan were determined by western blot analysis. MRI results indicated that intervertebral disc degeneration was ameliorated in the experimental group when compared with the control and the puncture group. Furthermore, the expression levels of type II collagen and aggrecan in the puncture and control groups were significantly lower than in the experimental group (P<0.05). In conclusion, lenti‑TGFβ3‑P2A‑CTGF‑T2A‑TIMP1 co‑transduction can promote synthesis of aggrecan and type II collagen in degenerative intervertebral discs, thereby delaying intervertebral disc degeneration. These results indicate the potential of gene therapy in treatment of intervertebral disc degeneration.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Yu T, Ma XX, Xiang HF, Hu YG and Chen BH: Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model. Exp Ther Med 11: 1399-1404, 2016.
APA
Liu, Y., Yu, T., Ma, X., Xiang, H., Hu, Y., & Chen, B. (2016). Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model. Experimental and Therapeutic Medicine, 11, 1399-1404. https://doi.org/10.3892/etm.2016.3063
MLA
Liu, Y., Yu, T., Ma, X., Xiang, H., Hu, Y., Chen, B."Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model". Experimental and Therapeutic Medicine 11.4 (2016): 1399-1404.
Chicago
Liu, Y., Yu, T., Ma, X., Xiang, H., Hu, Y., Chen, B."Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1399-1404. https://doi.org/10.3892/etm.2016.3063
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Yu T, Ma XX, Xiang HF, Hu YG and Chen BH: Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model. Exp Ther Med 11: 1399-1404, 2016.
APA
Liu, Y., Yu, T., Ma, X., Xiang, H., Hu, Y., & Chen, B. (2016). Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model. Experimental and Therapeutic Medicine, 11, 1399-1404. https://doi.org/10.3892/etm.2016.3063
MLA
Liu, Y., Yu, T., Ma, X., Xiang, H., Hu, Y., Chen, B."Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model". Experimental and Therapeutic Medicine 11.4 (2016): 1399-1404.
Chicago
Liu, Y., Yu, T., Ma, X., Xiang, H., Hu, Y., Chen, B."Lentivirus-mediated TGF-β3, CTGF and TIMP1 gene transduction as a gene therapy for intervertebral disc degeneration in an in vivo rabbit model". Experimental and Therapeutic Medicine 11, no. 4 (2016): 1399-1404. https://doi.org/10.3892/etm.2016.3063
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