Possible involvement of AMP‑activated protein kinase in PGE1‑induced synthesis of osteoprotegerin in osteoblasts

  • Authors:
    • Shingo Kainuma
    • Takanobu Otsuka
    • Gen Kuroyanagi
    • Naohiro Yamamoto
    • Rie Matsushima‑Nishiwaki
    • Osamu Kozawa
    • Haruhiko Tokuda
  • View Affiliations

  • Published online on: February 22, 2016     https://doi.org/10.3892/etm.2016.3099
  • Pages: 2042-2048
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Abstract

AMP‑activated protein kinase (AMPK) is firmly established as a central regulator of cellular energy homeostasis. We have previously reported that prostaglandin E1 (PGE1) stimulates the synthesis of osteoprotegerin through p38 mitogen‑activated protein (MAP) kinase and stress‑activated protein kinase/c‑Jun N‑terminal kinase (SAPK/JNK) in osteoblast‑like MC3T3‑E1 cells. The present study investigated the involvement of AMPK in PGE1‑induced osteoprotegerin synthesis in MC3T3‑E1 cells. The levels of osteoprotegerin were measured using an enzyme‑linked immunosorbent assay, while the phosphorylation of AMPK, acetyl‑CoA carboxylase, p38 MAP kinase and SAPK/JNK were analyzed by western blotting. In addition, the mRNA expression levels of osteoprotegerin were determined by a reverse transcription‑quantitative polymerase chain reaction. It was revealed that PGE1 significantly induced the phosphorylation of the α and β subunits of AMPK in a time‑dependent manner (P<0.05). In addition, acetyl‑CoA carboxylase, a direct substrate of AMPK, was significantly phosphorylated by PGE1 (P<0.05). Compound C, an AMPK inhibitor, was revealed to suppress the phosphorylation of acetyl‑CoA carboxylase, which significantly reduced the release and mRNA expression levels of PGE1‑stimulated osteoprotegerin (P<0.05). However, the PGE1‑induced phosphorylation of p38 MAP kinase and SAPK/JNK were not affected by compound C. The results of the present study indicated that AMPK may positively regulate PGE1‑stimulated osteoprotegerin synthesis in osteoblasts; thus providing novel insight into the regulatory mechanisms underlying bone metabolism.
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May-2016
Volume 11 Issue 5

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Kainuma S, Otsuka T, Kuroyanagi G, Yamamoto N, Matsushima‑Nishiwaki R, Kozawa O and Tokuda H: Possible involvement of AMP‑activated protein kinase in PGE1‑induced synthesis of osteoprotegerin in osteoblasts. Exp Ther Med 11: 2042-2048, 2016
APA
Kainuma, S., Otsuka, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Kozawa, O., & Tokuda, H. (2016). Possible involvement of AMP‑activated protein kinase in PGE1‑induced synthesis of osteoprotegerin in osteoblasts. Experimental and Therapeutic Medicine, 11, 2042-2048. https://doi.org/10.3892/etm.2016.3099
MLA
Kainuma, S., Otsuka, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Possible involvement of AMP‑activated protein kinase in PGE1‑induced synthesis of osteoprotegerin in osteoblasts". Experimental and Therapeutic Medicine 11.5 (2016): 2042-2048.
Chicago
Kainuma, S., Otsuka, T., Kuroyanagi, G., Yamamoto, N., Matsushima‑Nishiwaki, R., Kozawa, O., Tokuda, H."Possible involvement of AMP‑activated protein kinase in PGE1‑induced synthesis of osteoprotegerin in osteoblasts". Experimental and Therapeutic Medicine 11, no. 5 (2016): 2042-2048. https://doi.org/10.3892/etm.2016.3099