Efficient inhibition of human immunodeficiency virus replication using novel modified microRNA-30a targeting 3'-untranslated region transcripts

Nejati,Ahmad; Shahmahmoodi,Shohreh; Arefian,Ehsan; Shoja,Zabihollah; Marashi,Sayed‑Mahdi; Tabatabaie,Hamideh; Mollaei‑Kandelous,Yaghoub; Soleimani,Masoud; Nategh,Rakhshandeh

doi:10.3892/etm.2016.3121

Efficient inhibition of human immunodeficiency virus replication using novel modified microRNA-30a targeting 3'-untranslated region transcripts

Authors:
- Ahmad Nejati
- Shohreh Shahmahmoodi
- Ehsan Arefian
- Zabihollah Shoja
- Sayed‑Mahdi Marashi
- Hamideh Tabatabaie
- Yaghoub Mollaei‑Kandelous
- Masoud Soleimani
- Rakhshandeh Nategh
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Affiliations: Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran 14716‑13151, Iran, Biotechnology Center, College of Science, University of Tehran, Tehran 14176‑14411, Iran, Virology Department, Pasteur Institute of Iran, Tehran 13169‑43551, Iran, Immunology Department, Iran University of Medical Science, Tehran 14716‑13151, Iran, Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, Tehran 19977‑75555, Iran
Published online on: March 2, 2016 https://doi.org/10.3892/etm.2016.3121
Pages: 1833-1838

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Abstract

RNA interference (RNAi)-based gene therapy is currently considered to be a combinatorial anti-human immunodeficiency virus-1 (HIV-1) therapy. Although artificial polycistronic microRNAs (miRs) can reduce HIV‑1 escape mutant variants, this approach may increase the risk of side effects. The present study aimed to optimize the efficiency of anti-HIV RNAi gene therapy in order to reduce the cell toxicity induced by multi-short hairpin RNA expression. An artificial miR-30a-3'-untranslated region (miR-3'‑UTR) obtained from a single RNA polymerase II was used to simultaneously target all viral transcripts. The results of the present study demonstrated that HIV-1 replication was significantly inhibited in the cells with the miR-3'-UTR construct, suggesting that miR-3'-UTR may serve as a promising tool for RNAi-based gene therapy in the treatment of HIV-1.

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