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Article

Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model

  • Authors:
    • Ying Chen
    • Xianfei Hong
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, Jinhua Traditional Chinese Medical Hospital, Jinhua, Zhejiang 321000, P.R. China
  • Pages: 1987-1992
    |
    Published online on: March 10, 2016
       https://doi.org/10.3892/etm.2016.3140
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Abstract

Carvedilol is a novel third generation β-blocker that acts as an antagonist of β and α adrenergic receptors, and is able to regulate various cell factors. In addition, it possesses antioxidant activity, is capable of reversing cardiac remodeling effects and has anti-arrhythmic effects. The present study aimed to investigate whether the effects of carvedilol were able to reduce conjunctivitis clinical scores. Initially, 24 Sprague Dawley (SD) rats were randomly divided into three equal groups as follows: Control group, model group and carvedilol group. The model and carvedilol group adult SD rats were injected with lipopolysaccharide (LPS) to induce conjunctivitis. In the carvedilol group, the eight SD rats with LPS‑induced conjunctivitis also received 50 mg/kg/day of carvedilol for 4 weeks. Next, the effects carvedilol were assessed utilizing a system of clinical sign scores, and an enzyme‑linked immunosorbent assay was used to determine the expression levels of interleukin‑1β (IL‑1β), IL‑6, IL‑8 and tumor necrosis factor-α (TNF-α). Finally, nuclear factor‑κB (NF‑κB), nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) were analyzed by western blotting. Carvedilol was observed to significantly reduce clinical sign scores in a dose‑dependent manner (P<0.01), and reduce IL‑1β, IL‑6, IL‑8 and TNF‑α expression levels (P<0.01) in the LPS‑induced rat model of conjunctivitis. Carvedilol was also able to significantly reduce the protein expression levels of NF-κB, and induce the protein expression levels of NGF and VEGF in the LPS‑induced rat model of conjunctivitis (P<0.01). In conclusion, the effects of carvedilol may reduce conjunctivitis clinical scores through inflammation, NGF and VEGF in LPS-induced rat models.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y and Hong X: Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model. Exp Ther Med 11: 1987-1992, 2016.
APA
Chen, Y., & Hong, X. (2016). Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model. Experimental and Therapeutic Medicine, 11, 1987-1992. https://doi.org/10.3892/etm.2016.3140
MLA
Chen, Y., Hong, X."Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model". Experimental and Therapeutic Medicine 11.5 (2016): 1987-1992.
Chicago
Chen, Y., Hong, X."Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1987-1992. https://doi.org/10.3892/etm.2016.3140
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y and Hong X: Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model. Exp Ther Med 11: 1987-1992, 2016.
APA
Chen, Y., & Hong, X. (2016). Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model. Experimental and Therapeutic Medicine, 11, 1987-1992. https://doi.org/10.3892/etm.2016.3140
MLA
Chen, Y., Hong, X."Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model". Experimental and Therapeutic Medicine 11.5 (2016): 1987-1992.
Chicago
Chen, Y., Hong, X."Effects of carvedilol reduce conjunctivitis through changes in inflammation, NGF and VEGF levels in a rat model". Experimental and Therapeutic Medicine 11, no. 5 (2016): 1987-1992. https://doi.org/10.3892/etm.2016.3140
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