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Article

Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells

  • Authors:
    • Xiao Chen
    • Dong‑Dong Wang
    • Tong Wei
    • Su‑Mei He
    • Guan‑Ying Zhang
    • Qun‑Li Wei
  • View Affiliations / Copyright

    Affiliations: Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou, Jiangsu 221004, P.R. China
  • Pages: 2561-2566
    |
    Published online on: March 24, 2016
       https://doi.org/10.3892/etm.2016.3194
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Abstract

Diabetic nephropathy (DN) exhibits a deteriorating course that may lead to end‑stage renal failure. Astragalosides have been clinically tested for the treatment of DN, but the mechanism is unclear at present. In this study, the effects of astragalosides were investigated on high glucose‑induced proliferation and expression of transforming growth factor‑β1 (TGF‑β1), connective tissue growth factor (CTGF), type IV collagen (colIV) and fibronectin (FN) in glomerular mesangial cells (MCs). Cell proliferation was determined by 5‑bromo‑2'‑deoxyuridine assay, and the expression of TGF‑β1, CTGF, colIV and FN mRNA and proteins in MCs was detected by reverse transcription‑polymerase chain reaction and ELISA assay, respectively. The results showed that high glucose clearly induced the proliferation of MCs and increased the expression of TGF‑β1, CTGF, colIV and FN. Treatment with 50, 100, 200 µg/ml astragalosides inhibited cell proliferation and the expression of TGF‑β1, CTGF, colIV and FN induced by high glucose. Thus, it is concluded that astragalosides inhibit the increased cell proliferation and expression of major extracellular matrix proteins that are induced by high glucose, indicating their value for the prophylaxis and therapy of DN.
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1 

Ritz E and Orth SR: Nephropathy in patients with type 2 diabetes mellitus. N Engl J Med. 341:1127–1133. 1999. View Article : Google Scholar : PubMed/NCBI

2 

Remuzzi G, Schieppati A and Ruggenenti P: Clinical practice: Nephropathy in patients with type 2 diabetes. N Engl J Med. 346:1145–1151. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Kolset SO, Reinholt FP and Jenssen T: Diabetic nephropathy and extracellular matrix. J Histochem Cytochem. 60:976–986. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Tervaert TW, Mooyaart AL, Amann K, Cohen AH, Cook HT, Tervaert TW, Mooyaart AL, Amann K, Cohen AH, Cook HT, Drachenberg CB, Ferrario F, Fogo AB, Haas M, de Heer E, et al: Pathologic classification of diabetic nephropathy. J Am Soc Nephrol. 21:556–563. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Phillips CA and Molitch ME: The relationship between glucose control and the development and progression of diabetic nephropathy. Curr Diab Rep. 2:523–529. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Mishra R, Emancipator SN, Kern T and Simonson MS: High glucose evokes an intrinsic proapoptotic signaling pathway in mesangial cells. Kidney Int. 67:82–93. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Cooper ME: Interaction of metabolic and haemodynamic factors in mediating experimental diabetic nephropathy. Diabetologia. 44:1957–1972. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Ha H and Lee HB: Reactive oxygen species as glucose signaling molecules in mesangial cells cultured under high glucose. Kidney Int Suppl. 77:S19–S25. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Gao Q, Qin WS, Jia ZH, Zheng JM, Zeng CH, Li LS and Liu ZH: Rhein improves renal lesion and ameliorates dyslipidemia in db/db mice with diabetic nephropathy. Planta Med. 76:27–33. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Brosius FC III: New insights into the mechanisms of fibrosis and sclerosis in diabetic nephropathy. Rev Endocr Metab Disord. 9:245–254. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Wang JY, Yin XX, Wu YM, Tang DQ, Gao YY, Wan MR, Hou XY and Zhang B: Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells. Acta Pharmcol Sin. 27:1222–1230. 2006. View Article : Google Scholar

12 

Lal MA, Brismar H, Eklöf AC and Aperia A: Role of oxidative stress in advanced glycation end product-induced mesangial cells activation. Kidney Int. 61:2006–2014. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Dronavalli S, Duka I and Bakris GL: The pathogenesis of diabetic nephropathy. Nat Clin Pract Endocrinol Metab. 4:444–452. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Wang JY, Yin XX, Wu YM, Tang DQ, Gao YY, Wan MR, Hou XY and Zhang B: Ginkgo biloba extract suppresses hypertrophy and extracellular matrix accumulation in rat mesangial cells. Acta Pharmacol Sin. 27:1222–1230. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Xu W, Shao X, Tian L, Gu L, Zhang M, Wang Q, Wu B, Wang L, Yao J, Xu X, et al: Astragaloside IV ameliorates renal fibrosis via the inhibition of mitogen-activated protein kinases and antiapoptosis in vivo and in vitro. J Pharmacol Exp Ther. 350:552–562. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Sato S, Kawamura H, Takemoto M, Maezawa Y, Fujimoto M, Shimoyama T, Koshizaka M, Tsurutani Y, Watanabe A, Ueda S, et al: Halofuginone prevents extracellular matrix deposition in diabetic nephropathy. Biochem Biophys Res Commun. 379:411–416. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Rios JL and Waterman PG: A review of the pharmacology and toxicology of Astragalus. Phytother Res. 11:411–418. 1997. View Article : Google Scholar

18 

Liu G, Song J, Guo Y, Wang T and Zhou Z: Astragalus injection protects cerebral ischemic injury by inhibiting neuronal apoptosis and the expression of JNK3 after cerebral ischemic reperfusion in rats. Behav Brain Funct. 9:362013. View Article : Google Scholar : PubMed/NCBI

19 

Wei XP, Wu ZL and Wang JT: Clinical research on treating diabetes with Astragalus injection and TMP injection. Zhongguo Lin Chuang Yan Jiu. 6:142014.(In Chinese).

20 

Xu M, Yin J, Xie L, Zhang J, Zou C, Zou J, Liu F, Ju W and Li P: Pharmacokinetics and tolerance of total astragalosides after intravenous infusion of astragalosides injection in healthy Chinese volunteers. Phytomedicine. 20:1105–1111. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Mason RM and Wahab NA: Extracellular matrix metabolism in diabetic nephropathy. J Am Soc Nephrol. 14:1358–1373. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Nakajima T, Haseqawa G, Kamiuchi K, Fukui M, Yamasaki M, Tominaga M, Asano M, Hosoda H, Yoshikawa T and Nakamura N: Differential regulation of intracellular redox state by extracellular matrix proteins in glomerular mesangial cells: Potential role in diabetic nephropathy. Redox Rep. 11:223–230. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Sakharova OV, Taal MW and Brenner BM: Pathogenesis of diabetic nephropathy: Focus on transforming growth factor-beta and connective tissue growth factor. Curr Opin Nephrol Hypertens. 10:727–738. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Zhao TT, Zhang HJ, Lu XG, Huang XR, Zhang WK, Wang H, Lan HY and Li P: Chaihuang-Yishen granule inhibits diabetic kidney disease in rats through blocking TGF-β/Smad3 signaling. PLoS One. 9:e908072014. View Article : Google Scholar : PubMed/NCBI

25 

Sharma K and Ziyadeh FN: Renal hypertrophy is associated with upregulation of TGF-beta 1 gene expression in diabetic BB rat and NOD mouse. Am J Physiol. 267:F1094–F1101. 1994.PubMed/NCBI

26 

Border WA and Noble NA: Evidence that TGF-beta should be a therapeutic target in diabetic nephropathy. Kidney Int. 54:1390–1391. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Liu X, Luo F, Pan K, Wu W and Chen H: High glucose upregulates connective tissue growth factor expression in human vascular smooth muscle cells. BMC Cell Biol. 8:12007. View Article : Google Scholar : PubMed/NCBI

28 

Guha M, Xu ZG, Tung D, Lanting L and Natarajan R: Specific down-regulation of connective tissue growth factor attenuates progression of nephropathy in mouse models of type 1 and type 2 diabetes. FASEB J. 21:3355–3368. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Qi W, Chen X, Poronnik P and Pollock CA: Transforming growth factor-beta/connective tissue growth factor axis in the kidney. Int J Biochem Cell Biol. 40:9–13. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen X, Wang DD, Wei T, He SM, Zhang GY and Wei QL: Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells. Exp Ther Med 11: 2561-2566, 2016.
APA
Chen, X., Wang, D., Wei, T., He, S., Zhang, G., & Wei, Q. (2016). Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells. Experimental and Therapeutic Medicine, 11, 2561-2566. https://doi.org/10.3892/etm.2016.3194
MLA
Chen, X., Wang, D., Wei, T., He, S., Zhang, G., Wei, Q."Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells". Experimental and Therapeutic Medicine 11.6 (2016): 2561-2566.
Chicago
Chen, X., Wang, D., Wei, T., He, S., Zhang, G., Wei, Q."Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2561-2566. https://doi.org/10.3892/etm.2016.3194
Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Wang DD, Wei T, He SM, Zhang GY and Wei QL: Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells. Exp Ther Med 11: 2561-2566, 2016.
APA
Chen, X., Wang, D., Wei, T., He, S., Zhang, G., & Wei, Q. (2016). Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells. Experimental and Therapeutic Medicine, 11, 2561-2566. https://doi.org/10.3892/etm.2016.3194
MLA
Chen, X., Wang, D., Wei, T., He, S., Zhang, G., Wei, Q."Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells". Experimental and Therapeutic Medicine 11.6 (2016): 2561-2566.
Chicago
Chen, X., Wang, D., Wei, T., He, S., Zhang, G., Wei, Q."Effects of astragalosides from Radix Astragali on high glucose‑induced proliferation and extracellular matrix accumulation in glomerular mesangial cells". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2561-2566. https://doi.org/10.3892/etm.2016.3194
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