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Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis

  • Authors:
    • Meng Li
    • Quan Sun
    • Shengli Li
    • Yanan Zhai
    • Jingjing Wang
    • Baian Chen
    • Jing Lu
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Animal Science, School of Basic Medical Science, Capital Medical University, Beijing 100069, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2147-2152
    |
    Published online on: March 30, 2016
       https://doi.org/10.3892/etm.2016.3205
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Abstract

Stress as a cofactor has been reported to affect the progression and severity of liver diseases. The present study investigated the effect of chronic restraint stress on carbon tetrachloride (CCl4)‑induced liver fibrosis. A total of 30 male BALB/c mice were randomly divided into three groups: Oil‑treated control group; CCl4‑treated group; and CCl4 + restraint‑treated group. CCl4 was administrated via intraperitoneal injection once every 3 days over a period of 42 days. In the CCl4 + restraint‑treated group, mice were immobilized using 50 ml centrifuge tubes for 0.5 h to inflict chronic restraint stress immediately after the injection of CCl4. On day 42, blood and liver tissue samples were collected for analysis. The effect of restraint on CCl4‑induced liver fibrosis in mice was evaluated by analyzing the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Histopathological examination of liver samples was performed using hematoxylin and eosin (HE), Masson's trichrome, 5‑hydroxytryptamine 2B (5‑HT2B) receptor and α‑smooth muscle actin (α‑SMA) immumohistochemical staining. ALT, AST, 5‑HT2B receptor and α‑SMA expression levels were significantly increased in mice exposed to CCl4 in comparison with those in the oil‑treated control mice (P<0.01). However, these increases were significantly reduced by exposure to restraint (P<0.05). HE and Masson's trichrome staining revealed that restraint can alleviate CCl4‑induced liver fibrosis. These results suggest that chronic restraint stress reduces the development of liver fibrosis by inhibiting the activation of hepatic stellate cells via 5‑HT2B receptor. Therefore, restraint may be a useful therapeutic approach in the management of liver fibrosis.
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Copy and paste a formatted citation
Spandidos Publications style
Li M, Sun Q, Li S, Zhai Y, Wang J, Chen B and Lu J: Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis. Exp Ther Med 11: 2147-2152, 2016.
APA
Li, M., Sun, Q., Li, S., Zhai, Y., Wang, J., Chen, B., & Lu, J. (2016). Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis. Experimental and Therapeutic Medicine, 11, 2147-2152. https://doi.org/10.3892/etm.2016.3205
MLA
Li, M., Sun, Q., Li, S., Zhai, Y., Wang, J., Chen, B., Lu, J."Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis". Experimental and Therapeutic Medicine 11.6 (2016): 2147-2152.
Chicago
Li, M., Sun, Q., Li, S., Zhai, Y., Wang, J., Chen, B., Lu, J."Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2147-2152. https://doi.org/10.3892/etm.2016.3205
Copy and paste a formatted citation
x
Spandidos Publications style
Li M, Sun Q, Li S, Zhai Y, Wang J, Chen B and Lu J: Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis. Exp Ther Med 11: 2147-2152, 2016.
APA
Li, M., Sun, Q., Li, S., Zhai, Y., Wang, J., Chen, B., & Lu, J. (2016). Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis. Experimental and Therapeutic Medicine, 11, 2147-2152. https://doi.org/10.3892/etm.2016.3205
MLA
Li, M., Sun, Q., Li, S., Zhai, Y., Wang, J., Chen, B., Lu, J."Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis". Experimental and Therapeutic Medicine 11.6 (2016): 2147-2152.
Chicago
Li, M., Sun, Q., Li, S., Zhai, Y., Wang, J., Chen, B., Lu, J."Chronic restraint stress reduces carbon tetrachloride‑induced liver fibrosis". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2147-2152. https://doi.org/10.3892/etm.2016.3205
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