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Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds

  • Authors:
    • Songsong Teng
    • Chaoxu Liu
    • Daniel Guenther
    • Mohamed Omar
    • Claudia Neunaber
    • Christian Krettek
    • Michael Jagodzinski
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Trauma, Hannover Medical School, 30625 Hannover, Germany
    Copyright: © Teng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2086-2094
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    Published online on: March 30, 2016
       https://doi.org/10.3892/etm.2016.3206
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Abstract

The aim of the present investigation was to compare the effects of cyclic compression, perfusion, dexamethasone (DEX) and bone morphogenetic protein‑7 (BMP‑7) on the proliferation and differentiation of human bone marrow stromal cells (hBMSCs) in polyurethane scaffolds in a perfusion bioreactor. Polyurethane scaffolds seeded with hBMSCs were cultured under six different conditions, as follows: 10% Cyclic compression at 0.5 and 5 Hz; 10 ml/min perfusion; 100 nM DEX; 100 ng/ml BMP‑7; and 1 ml/min perfusion without mechanical and biochemical stimulation (control). On days 7 and 14, samples were tested for the following data: Cell proliferation; mRNA expression of Runx2, COL1A1 and osteocalcin; osteocalcin content; calcium deposition; and the equilibrium modulus of the tissue specimen. The results indicated that BMP‑7 and 10 ml/min perfusion promoted cell proliferation, which was inhibited by 5 Hz cyclic compression and DEX. On day 7, the 5 Hz cyclic compression inhibited Runx2 expression, whereas the 0.5 Hz cyclic compression and BMP‑7 upregulated the COL1A1 mRNA levels on day 7 and enhanced the osteocalcin expression on day 14. The DEX‑treated hBMSCs exhibited downregulated osteocalcin expression. After 14 days, the BMP‑7 group exhibited the highest calcium deposition, followed by the 0.5 Hz cyclic compression and the DEX groups. The equilibrium modulus of the engineered constructs significantly increased in the BMP‑7, 0.5 Hz cyclic compression and DEX groups. In conclusion, the present results suggest that BMP‑7 and perfusion enhance cell proliferation, whereas high frequency cyclic compression inhibits the proliferation and osteogenic differentiation of hBMSCs. Low frequency cyclic compression is more effective than DEX, but less effective compared with BMP‑7 on the osteogenic differentiation of hBMSCs seeded on polyurethane scaffolds.
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View References

1 

Murphy CM, O'Brien FJ, Little DG and Schindeler A: Cell-scaffold interactions in the bone tissue engineering triad. Eur Cell Mater. 26:120–132. 2013.PubMed/NCBI

2 

Colnot C: Cell sources for bone tissue engineering: Insights from basic science. Tissue Eng Part B Rev. 17:449–457. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Wang YK and Chen CS: Cell adhesion and mechanical stimulation in the regulation of mesenchymal stem cell differentiation. J Cell Mol Med. 17:823–832. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Langenbach F and Handschel J: Effects of dexamethasone, ascorbic acid and beta-glycerophosphate on the osteogenic differentiation of stem cells in vitro. Stem Cell Res Ther. 4:1172013. View Article : Google Scholar : PubMed/NCBI

5 

Sittichokechaiwut A, Edwards JH, Scutt AM and Reilly GC: Short bouts of mechanical loading are as effective as dexamethasone at inducing matrix production by human bone marrow mesenchymal stem cell. Eur Cell Mater. 20:45–57. 2010.PubMed/NCBI

6 

Li R, Liang L, Dou Y, Huang Z, Mo H, Wang Y and Yu B: Mechanical strain regulates osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells. Biomed Res Int. 2015:8732512015.PubMed/NCBI

7 

Cardwell RD, Kluge JA, Thayer PS, Guelcher SA, Dahlgren LA, Kaplan DL and Goldstein AS: Static and cyclic mechanical loading of mesenchymal stem cells on elastomeric, electrospun polyurethane meshes. J Biomech Eng. 137:2015. View Article : Google Scholar : PubMed/NCBI

8 

Kim TJ, Joo C, Seong J, Vafabakhsh R, Botvinick EL, Berns MW, Palmer AE, Wang N, Ha T, Jakobsson E, et al: Distinct mechanisms regulating mechanical force-induced Ca2+ signals at the plasma membrane and the ER in human MSCs. eLife. 4:e048762015. View Article : Google Scholar : PubMed/NCBI

9 

Delaine-Smith RM, MacNeil S and Reilly GC: Matrix production and collagen structure are enhanced in two types of osteogenic progenitor cells by a simple fluid shear stress stimulus. Eur Cell Mater. 24:162–174. 2012.PubMed/NCBI

10 

Yourek G, McCormick SM, Mao JJ and Reilly GC: Shear stress induces osteogenic differentiation of human mesenchymal stem cells. Regen Med. 5:713–724. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Liu Y, Wu J, Zhu Y and Han J: Therapeutic application of mesenchymal stem cells in bone and joint diseases. Clin Exp Med. 14:13–24. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Gautschi OP, Frey SP and Zellweger R: Bone morphogenetic proteins in clinical applications. ANZ J Surg. 77:626–631. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Lee KB, Taghavi CE, Murray SS, Song KJ, Keorochana G and Wang JC: BMP induced inflammation: A comparison of rhBMP-7 and rhBMP-2. J Orthop Res. 30:1985–1994. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Weiskirchen R and Meurer SK: BMP-7 counteracting TGF-beta1 activities in organ fibrosis. Front Biosci (Landmark Ed). 18:1407–1434. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Black CR, Goriainov V, Gibbs D, Kanczler J, Tare RS and Oreffo RO: Bone Tissue Engineering. Curr Mol Biol Rep. 1:132–140. 2015. View Article : Google Scholar : PubMed/NCBI

16 

Gong T, Xie J, Liao J, Zhang T, Lin S and Lin Y: Nanomaterials and bone regeneration. Bone Res. 3:150292015. View Article : Google Scholar : PubMed/NCBI

17 

Liu C, Abedian R, Meister R, Haasper C, Hurschler C, Krettek C, von Lewinski G and Jagodzinski M: Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds. Biomaterials. 33:1052–1064. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Verdonk P, Beaufils P, Bellemans J, Djian P, Heinrichs EL, Huysse W, Laprell H, Siebold R and Verdonk R: Actifit Study Group: Successful treatment of painful irreparable partial meniscal defects with a polyurethane scaffold: Two-year safety and clinical outcomes. Am J Sports Med. 40:844–853. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Hannink G, de Mulder EL, van Tienen TG and Buma P: Effect of load on the repair of osteochondral defects using a porous polymer scaffold. J Biomed Mater Res B Appl Biomater. 100:2082–2089. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Steinert AF, Rackwitz L, Gilbert F, Noth U and Tuan RS: Concise review: the clinical application of mesenchymal stem cells for musculoskeletal regeneration: current status and perspectives. Stem Cells Transl Med. 1:237–247. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Jagodzinski M, Drescher M, Zeichen J, Hankemeier S, Krettek C, Bosch U and van Griensven M: Effects of cyclic longitudinal mechanical strain and dexamethasone on osteogenic differentiation of human bone marrow stromal cells. Eur Cell Mater. 7:35–41, Discussion 41. 2004.PubMed/NCBI

22 

van Tienen TG, Heijkants RG, Buma P, de Groot JH, Pennings AJ and Veth RP: Tissue ingrowth and degradation of two biodegradable porous polymers with different porosities and pore sizes. Biomaterials. 23:1731–1738. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Jagodzinski M, Breitbart A, Wehmeier M, Hesse E, Haasper C, Krettek C, Zeichen J and Hankemeier S: Influence of perfusion and cyclic compression on proliferation and differentiation of bone marrow stromal cells in 3-dimensional culture. J Biomech. 41:1885–1891. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Liu J, Zhang B, Song S, Ma M, Si S, Wang Y, Xu B, Feng K, Wu J and Guo Y: Bovine collagen peptides compounds promote the proliferation and differentiation of MC3T3-E1 pre-osteoblasts. PloS One. 9:e999202014. View Article : Google Scholar : PubMed/NCBI

26 

Kelly DJ, Crawford A, Dickinson SC, Sims TJ, Mundy J, Hollander AP, Prendergast PJ and Hatton PV: Biochemical markers of the mechanical quality of engineered hyaline cartilage. J Mater Sci Mater Med. 18:273–281. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Skerry TM: The response of bone to mechanical loading and disuse: fundamental principles and influences on osteoblast/osteocyte homeostasis. Arch Biochem Biophys. 473:117–123. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Turner CH, Owan I, Alvey T, Hulman J and Hock JM: Recruitment and proliferative responses of osteoblasts after mechanical loading in vivo determined using sustained-release bromodeoxyuridine. Bone. 22:463–469. 1998. View Article : Google Scholar : PubMed/NCBI

29 

Jones LC, Yeoumans B, Hungerford DS and Frondoza CG: The response of osteoblast-like cells to dexamethasone and cyclic loading. Biomed Sci Instrum. 42:273–277. 2006.PubMed/NCBI

30 

Makhdom AM and Hamdy RC: The role of growth factors on acceleration of bone regeneration during distraction osteogenesis. Tissue Eng Part B Rev. 19:442–453. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Huang C, Dai J and Zhang XA: Environmental physical cues determine the lineage specification of mesenchymal stem cells. Biochim Biophys Acta. 1850:1261–1266. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Michalopoulos E, Knight RL, Korossis S, Kearney JN, Fisher J and Ingham E: Development of methods for studying the differentiation of human mesenchymal stem cells under cyclic compressive strain. Tissue Eng Part C Methods. 18:252–262. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Delaine-Smith RM and Reilly GC: Mesenchymal stem cell responses to mechanical stimuli. Muscles Ligaments Tendons J. 2:169–180. 2012.PubMed/NCBI

34 

Murphy CM, Haugh MG and O'Brien FJ: The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering. Biomaterials. 31:461–466. 2010. View Article : Google Scholar : PubMed/NCBI

35 

Karageorgiou V and Kaplan D: Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 26:5474–5491. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Roosa SM, Kemppainen JM, Moffitt EN, Krebsbach PH and Hollister SJ: The pore size of polycaprolactone scaffolds has limited influence on bone regeneration in an in vivo model. J Biomed Mater Res A. 92:359–368. 2010. View Article : Google Scholar : PubMed/NCBI

37 

Kuboki Y, Jin Q and Takita H: Geometry of carriers controlling phenotypic expression in BMP-induced osteogenesis and chondrogenesis. J Bone Joint Surg Am. 83-A(Suppl 1): S105–S115. 2001.PubMed/NCBI

38 

Gorna K and Gogolewski S: Biodegradable porous polyurethane scaffolds for tissue repair and regeneration. J Biomed Mater Res A. 79:128–138. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Gaspar DA, Gomide V and Monteiro FJ: The role of perfusion bioreactors in bone tissue engineering. Biomatter. 2:167–175. 2012. View Article : Google Scholar : PubMed/NCBI

40 

Shea CM, Edgar CM, Einhorn TA and Gerstenfeld LC: BMP treatment of C3H10T1/2 mesenchymal stem cells induces both chondrogenesis and osteogenesis. J Cell Biochem. 90:1112–1127. 2003. View Article : Google Scholar : PubMed/NCBI

41 

Cheng SL, Yang JW, Rifas L, Zhang SF and Avioli LV: Differentiation of human bone marrow osteogenic stromal cells in vitro: Induction of the osteoblast phenotype by dexamethasone. Endocrinology. 134:277–286. 1994. View Article : Google Scholar : PubMed/NCBI

42 

Pelaez D, Huang CY and Cheung HS: Cyclic compression maintains viability and induces chondrogenesis of human mesenchymal stem cells in fibrin gel scaffolds. Stem Cells Dev. 18:93–102. 2009. View Article : Google Scholar : PubMed/NCBI

43 

Mathews S, Bhonde R, Gupta PK and Totey S: Novel biomimetic tripolymer scaffolds consisting of chitosan, collagen type 1 and hyaluronic acid for bone marrow-derived human mesenchymal stem cells-based bone tissue engineering. J Biomed Mater Res B Appl Biomater. 102:1825–1834. 2014. View Article : Google Scholar : PubMed/NCBI

44 

Kim K, Dean D, Mikos AG and Fisher JP: Effect of initial cell seeding density on early osteogenic signal expression of rat bone marrow stromal cells cultured on cross-linked poly(propylene fumarate) disks. Biomacromolecules. 10:1810–1817. 2009. View Article : Google Scholar : PubMed/NCBI

45 

Frank O, Heim M, Jakob M, Barbero A, Schäfer D, Bendik I, Dick W, Heberer M and Martin I: Real-time quantitative RT-PCR analysis of human bone marrow stromal cells during osteogenic differentiation in vitro. J Cell Biochem. 85:737–746. 2002. View Article : Google Scholar : PubMed/NCBI

46 

Xiao G, Wang D, Benson MD, Karsenty G and Franceschi RT: Role of the alpha2-integrin in osteoblast-specific gene expression and activation of the Osf2 transcription factor. J Biol Chem. 273:32988–32994. 1998. View Article : Google Scholar : PubMed/NCBI

47 

Fiorentini E, Granchi D, Leonardi E, Baldini N and Ciapetti G: Effects of osteogenic differentiation inducers on in vitro expanded adult mesenchymal stromal cells. Int J Artif Organs. 34:998–1011. 2011. View Article : Google Scholar : PubMed/NCBI

48 

Ito S, Suzuki N, Kato S, Takahashi T and Takagi M: Glucocorticoids induce the differentiation of a mesenchymal progenitor cell line, ROB-C26 into adipocytes and osteoblasts, but fail to induce terminal osteoblast differentiation. Bone. 40:84–92. 2007. View Article : Google Scholar : PubMed/NCBI

49 

Kim HJ, Kim UJ, Vunjak-Novakovic G, Min BH and Kaplan DL: Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells. Biomaterials. 26:4442–4452. 2005. View Article : Google Scholar : PubMed/NCBI

50 

Dahlin RL, Gershovich JG, Kasper FK and Mikos AG: Flow perfusion co-culture of human mesenchymal stem cells and endothelial cells on biodegradable polymer scaffolds. Ann Biomed Eng. 42:1381–1390. 2014. View Article : Google Scholar : PubMed/NCBI

51 

Kim J and Ma T: Bioreactor strategy in bone tissue engineering: Pre-culture and osteogenic differentiation under two flow configurations. Tissue Eng Part A. 18:2354–2364. 2012. View Article : Google Scholar : PubMed/NCBI

52 

Duty AO, Oest ME and Guldberg RE: Cyclic mechanical compression increases mineralization of cell-seeded polymer scaffolds in vivo. J Biomech Eng. 129:531–539. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Teng S, Liu C, Guenther D, Omar M, Neunaber C, Krettek C and Jagodzinski M: Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds. Exp Ther Med 11: 2086-2094, 2016.
APA
Teng, S., Liu, C., Guenther, D., Omar, M., Neunaber, C., Krettek, C., & Jagodzinski, M. (2016). Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds. Experimental and Therapeutic Medicine, 11, 2086-2094. https://doi.org/10.3892/etm.2016.3206
MLA
Teng, S., Liu, C., Guenther, D., Omar, M., Neunaber, C., Krettek, C., Jagodzinski, M."Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds". Experimental and Therapeutic Medicine 11.6 (2016): 2086-2094.
Chicago
Teng, S., Liu, C., Guenther, D., Omar, M., Neunaber, C., Krettek, C., Jagodzinski, M."Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2086-2094. https://doi.org/10.3892/etm.2016.3206
Copy and paste a formatted citation
x
Spandidos Publications style
Teng S, Liu C, Guenther D, Omar M, Neunaber C, Krettek C and Jagodzinski M: Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds. Exp Ther Med 11: 2086-2094, 2016.
APA
Teng, S., Liu, C., Guenther, D., Omar, M., Neunaber, C., Krettek, C., & Jagodzinski, M. (2016). Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds. Experimental and Therapeutic Medicine, 11, 2086-2094. https://doi.org/10.3892/etm.2016.3206
MLA
Teng, S., Liu, C., Guenther, D., Omar, M., Neunaber, C., Krettek, C., Jagodzinski, M."Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds". Experimental and Therapeutic Medicine 11.6 (2016): 2086-2094.
Chicago
Teng, S., Liu, C., Guenther, D., Omar, M., Neunaber, C., Krettek, C., Jagodzinski, M."Influence of biomechanical and biochemical stimulation on the proliferation and differentiation of bone marrow stromal cells seeded on polyurethane scaffolds". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2086-2094. https://doi.org/10.3892/etm.2016.3206
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