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Article

Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation

  • Authors:
    • Xiao‑Gang Liu
    • Hong‑Wei Hou
    • Yi‑Lin Liu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Weihai Hospital of Qingdao University, Weihai, Shandong 264200, P.R. China, Department of Information, Weihai Hospital of Qingdao University, Weihai, Shandong 264200, P.R. China, Department of Internal Medicine, Worker's Hospital of Xinjiang Dushanzi Mining Area Services Division, Karamay, Xinjiang 833699, P.R. China
  • Pages: 2333-2340
    |
    Published online on: April 11, 2016
       https://doi.org/10.3892/etm.2016.3250
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Abstract

The present study aimed to investigate the expression and roles of interleukin (IL)‑17 and tumor necrosis factor (TNF)‑α in intervertebral disc degeneration (IDD) and to identify the association between the effects of IL‑17 and TNF‑α in IDD. This may increase understanding of the pathogenic mechanism underlying IDD, and aid the development of alternative therapies. The experimental group consisted of 40 samples of nucleus pulposus tissue obtained from the intervertebral discs (IVDs) of patients with IDD by surgical intervention, and was further divided into an annulus fibrosus disrupted group, comprising 18 patients in which the external annulus was ruptured, and an annulus fibrosus intact group comprising 22 patients. The control group consisted of 20 samples of nucleus pulposus tissue from the IVDs of patients with traumatic lumbar disc fractures. The mRNA and protein expression levels of IL‑17 and TNF‑α in the 50 tissue samples were detected by semi‑quantitative reverse transcription polymerase chain reaction and immunohistochemical staining, respectively, and the results were statistically analyzed. The IL‑17 and TNF‑α protein and mRNA expression levels in the annulus fibrosus disrupted and annulus fibrosus intact groups were both higher compared with those in the control group. In addition, the expression levels of IL‑17 and TNF‑α in the annulus fibrosus disrupted group were significantly higher compared with those in the annulus fibrosus intact group (P<0.01). A positive correlation was identified between the mRNA and protein expression levels of IL‑17 and TNF‑α in the experimental group (r=0.957, P<0.01). IL‑17 and TNF‑α may therefore be involved in the progression of human IDD, and may have synergistic effects in the development of IDD.
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Copy and paste a formatted citation
Spandidos Publications style
Liu XG, Hou HW and Liu YL: Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation. Exp Ther Med 11: 2333-2340, 2016.
APA
Liu, X., Hou, H., & Liu, Y. (2016). Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation. Experimental and Therapeutic Medicine, 11, 2333-2340. https://doi.org/10.3892/etm.2016.3250
MLA
Liu, X., Hou, H., Liu, Y."Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation". Experimental and Therapeutic Medicine 11.6 (2016): 2333-2340.
Chicago
Liu, X., Hou, H., Liu, Y."Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2333-2340. https://doi.org/10.3892/etm.2016.3250
Copy and paste a formatted citation
x
Spandidos Publications style
Liu XG, Hou HW and Liu YL: Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation. Exp Ther Med 11: 2333-2340, 2016.
APA
Liu, X., Hou, H., & Liu, Y. (2016). Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation. Experimental and Therapeutic Medicine, 11, 2333-2340. https://doi.org/10.3892/etm.2016.3250
MLA
Liu, X., Hou, H., Liu, Y."Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation". Experimental and Therapeutic Medicine 11.6 (2016): 2333-2340.
Chicago
Liu, X., Hou, H., Liu, Y."Expression levels of IL‑17 and TNF‑α in degenerated lumbar intervertebral discs and their correlation". Experimental and Therapeutic Medicine 11, no. 6 (2016): 2333-2340. https://doi.org/10.3892/etm.2016.3250
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