Neuroprotective mechanism of HIF-1α overexpression in the early stage of acute cerebral infarction in rats

Sun,Yuhua; He,Weiya; Geng,Lijiao

doi:10.3892/etm.2016.3288

Neuroprotective mechanism of HIF-1α overexpression in the early stage of acute cerebral infarction in rats

Authors:
- Yuhua Sun
- Weiya He
- Lijiao Geng
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Affiliations: Department of Neurology, Henan University Huaihe Hospital, Kaifeng, Henan 475001, P.R. China
Published online on: April 21, 2016 https://doi.org/10.3892/etm.2016.3288
Pages: 391-395

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Abstract

The present study aimed to explore the expression and neuroprotective mechanism of hypoxia inducible factor (HIF‑1α) in the brain tissue of a rat model of early acute cerebral infarction. A total of 64 Sprague Dawley rats were randomly divided into surgery and sham groups and the model of focal cerebral infarction was established by the suture‑occluded method. In the sham group, blood vessels were separated but not occluded. Rats in the surgery and sham groups were subdivided into eight groups (n=4/group). Blood samples was collected at 8 time points including 30 min and 1, 3, 6, 12, 48, 24 and 72 h, respectively, and HIF‑1α content was detected using ELISA. Brain tissues of rats in all groups were harvested following blood collection. HIF‑1α protein expression was detected by immunohistochemistry and terminal deoxynucleotidyl transferase (TdT) dUTP nick‑end labeling was used to analyze the brain cell apoptosis index. ELISA results demonstrated that rats in the surgery group began to express HIF‑1α within 30 min, and HIF‑1α expression levels gradually increased, peaking at 12 h. HIF‑1α expression levels were significantly increased in the surgery group at all time points, as compared with the sham group (P<0.05). The concentration of HIF‑1α decreased rapidly in 12 h. At various time points, HIF‑1α protein expression in the brain tissue of rats in the sham group was negative. HIF‑1α protein expression was significantly increased in the surgery group (P<0.05), peaking at 12 h, and decreasing after this point. As compared with the sham group, the apoptosis indices of the brain tissue of rats in the surgery group exhibited a gradual increasing trend with significant decreases observed after 12 h (P<0.05). Intra‑group comparison of all indices in the surgery group, indicated that there was a statistically significant difference between postoperative 12 h and other time points (P<0.05). In conclusion, the present study demonstrated that HIF‑1α was highly expressed in the brain tissue of rat models of early acute cerebral infarction. The results also indicated that HIF‑1α significantly reduced the apoptosis of infarcted cells, suggesting that HIF‑1α may have a neuroprotective role in early acute cerebral infarction.

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