Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43

  • Authors:
    • Ying‑Yan Zhou
    • Run‑Yue Huang
    • Jie‑Hua Lin
    • Yong‑Yue Xu
    • Xiao‑Hong He
    • Yi‑Ting He
  • View Affiliations

  • Published online on: April 26, 2016     https://doi.org/10.3892/etm.2016.3292
  • Pages: 347-353
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Abstract

Bushen‑Qiangdu‑Zhilv (BQZ) decoction is a traditional Chinese medicinal compound widely used for treating ankylosing spondylitis (AS). However, the mechanisms underlying effects of BQZ remain largely unknown. Osteoblast differentiation of fibroblasts plays an important role in heterotopic ossification (HO) of AS, and connexin 43 (Cx43) is crucially involved in the osteoblast differentiation of fibroblasts. The aim of the present study was to evaluate the effects of BQZ on the osteogenic differentiation of fibroblasts by regulating Cx43. Rat fibroblasts were treated with freeze‑dried powder of BQZ, in the presence or absence of recombinant human bone morphogenetic protein‑2 (rhBMP‑2). MTS assays were performed to examine the inhibitory effects of BQZ on fibroblast proliferation. Western blot assays were conducted to detect the protein expression of core‑binding factor alpha 1 (Cbfα1), Cx43 and phosphorylated Cx43 (pCx43). BQZ appeared to inhibit fibroblast proliferation in a dose‑dependent manner. Furthermore, the expression of Cbfα1 and Cx43/pCx43 was significantly suppressed by BQZ, with or without rhBMP‑2 stimulation. Therefore, the present results indicate that BQZ may exert an anti‑AS effect by suppressing the osteogenic differentiation of fibroblasts via Cx43 regulation.
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July-2016
Volume 12 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhou YY, Huang RY, Lin JH, Xu YY, He XH and He YT: Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43. Exp Ther Med 12: 347-353, 2016
APA
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., & He, Y. (2016). Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43. Experimental and Therapeutic Medicine, 12, 347-353. https://doi.org/10.3892/etm.2016.3292
MLA
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., He, Y."Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43". Experimental and Therapeutic Medicine 12.1 (2016): 347-353.
Chicago
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., He, Y."Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43". Experimental and Therapeutic Medicine 12, no. 1 (2016): 347-353. https://doi.org/10.3892/etm.2016.3292