Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
July-2016 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2016 Volume 12 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43

  • Authors:
    • Ying‑Yan Zhou
    • Run‑Yue Huang
    • Jie‑Hua Lin
    • Yong‑Yue Xu
    • Xiao‑Hong He
    • Yi‑Ting He
  • View Affiliations / Copyright

    Affiliations: Department of Rheumatology, College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Department of Rheumatology, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China, Department of New Drug Development, The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China
  • Pages: 347-353
    |
    Published online on: April 26, 2016
       https://doi.org/10.3892/etm.2016.3292
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Bushen‑Qiangdu‑Zhilv (BQZ) decoction is a traditional Chinese medicinal compound widely used for treating ankylosing spondylitis (AS). However, the mechanisms underlying effects of BQZ remain largely unknown. Osteoblast differentiation of fibroblasts plays an important role in heterotopic ossification (HO) of AS, and connexin 43 (Cx43) is crucially involved in the osteoblast differentiation of fibroblasts. The aim of the present study was to evaluate the effects of BQZ on the osteogenic differentiation of fibroblasts by regulating Cx43. Rat fibroblasts were treated with freeze‑dried powder of BQZ, in the presence or absence of recombinant human bone morphogenetic protein‑2 (rhBMP‑2). MTS assays were performed to examine the inhibitory effects of BQZ on fibroblast proliferation. Western blot assays were conducted to detect the protein expression of core‑binding factor alpha 1 (Cbfα1), Cx43 and phosphorylated Cx43 (pCx43). BQZ appeared to inhibit fibroblast proliferation in a dose‑dependent manner. Furthermore, the expression of Cbfα1 and Cx43/pCx43 was significantly suppressed by BQZ, with or without rhBMP‑2 stimulation. Therefore, the present results indicate that BQZ may exert an anti‑AS effect by suppressing the osteogenic differentiation of fibroblasts via Cx43 regulation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Wendling D and Claudepierre P: New bone formation in axial spondyloarthritis. Joint Bone Spine. 80:454–458. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Xiang YJ and Dai SM: Prevalence of rheumatic diseases and disability in China. Rheumatol Int. 29:481–490. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Yu F, Cui Y, Zhou X, Zhang X and Han J: Osteogenic differentiation of human ligament fibroblasts induced by conditioned medium of osteoclast-like cells. Biosci Trends. 5:46–51. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Nishimura R, Hata K, Harris SE, Ikeda F and Yoneda T: Core-binding factor alpha 1 (Cbfa1) induces osteoblastic differentiation of C2C12 cells without interactions with Smad1 and Smad5. Bone. 31:303–312. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Kopf J, Petersen A, Duda GN and Knaus P: BMP2 and mechanical loading cooperatively regulate immediate early signalling events in the BMP pathway. BMC Biol. 10:372012. View Article : Google Scholar : PubMed/NCBI

6 

Zhang J and Wang JH: BMP-2 mediates PGE(2)-induced reduction of proliferation and osteogenic differentiation of human tendon stem cells. J Orthop Res. 30:47–52. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Chen HA, Chen CH, Lin YJ, Chen PC, Chen WS, Lu CL and Chou CT: Association of bone morphogenetic proteins with spinal fusion in ankylosing spondylitis. J Rheumatol. 37:2126–2132. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Hashida Y, Nakahama K, Shimizu K, Akiyama M, Harada K and Morita I: Communication-dependent mineralization of osteoblasts via gap junctions. Bone. 61:19–26. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Yang HS, Lu XH, Chen DY, Yuan W, Yang LL, He HL and Chen Y: Upregulated expression of connexin43 in spinal ligament fibroblasts derived from patients presenting ossification of the posterior longitudinal ligament. Spine (Phila Pa 1976). 36:2267–2274. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Haroon N, Kim TH and Inman RD: NSAIDs and radiographic progression in ankylosing spondylitis Bagging big game with small arms? Ann Rheum Dis. 71:1593–1595. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Braun J, van den Berg R, Baraliakos X, Boehm H, Burgos-Vargas R, Collantes-Estevez E, Dagfinrud H, Dijkmans B, Dougados M, Emery P, et al: 2010 update of the ASAS/EULAR recommendations for the management of ankylosing spondylitis. Ann Rheum Dis. 70:896–904. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Kristensen LE, Jakobsen AK, Askling J, Nielsson F and Jacobsson LT: Safety of etoricoxib, celecoxib, and nonselective Nonsteroidal Antiinflammatory Drugs in Ankylosing spondylitis and other Spondyloarthritis patients: A Swedish national population-based cohort study. Arthritis Care Res (Hoboken). 67:1137–1149. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Escalas C, Trijau S and Dougados M: Evaluation of the treatment effect of NSAIDs/TNF blockers according to different domains in ankylosing spondylitis: Results of a meta-analysis. Rheumatology (Oxford). 49:1317–1325. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Baraliakos X, Haibel H, Listing J, Sieper J and Braun J: Continuous long-term anti-TNF therapy does not lead to an increase in the rate of new bone formation over 8 years in patients with ankylosing spondylitis. Ann Rheum Dis. 73:710–715. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Zhang N, Zhang YZ, Tao QW and Yan XP: Treatment of ankylosing spondylitis by modified bushen zhuanggu recipe: A clinical observation. Zhongguo Zhong Xi Yi Jie He Za Zhi. 33:1611–1616. 2013.(In Chinese). PubMed/NCBI

16 

Huang RY, Lin JH, He XH, Li X, Lu CL, Zhou YY, Cai J and He YT: Anti-inflammatory activity of extracts of Bushen-Qiangdu-Zhilv decoction, a Chinese medicinal formula, in M1-polarized RAW264.7. BMC Complement Altern Med. 14:2682014. View Article : Google Scholar : PubMed/NCBI

17 

Garber JC BRBJ: Guide for the care and use of laboratory animals. United State: National Academies Press. 2011.PubMed/NCBI

18 

Men T, Piao SH and Teng CB: Regulation of differentiation of mesenchymal stem cells by the Hippo pathway effectors TAZ/YAP. Yi Chuan. 35:1283–1290. 2013.(In Chinese). View Article : Google Scholar : PubMed/NCBI

19 

Zouani OF, Rami L, Lei Y and Durrieu MC: Insights into the osteoblast precursor differentiation towards mature osteoblasts induced by continuous BMP-2 signaling. Biol Open. 2:872–881. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Umehara K, Iimura T, Sakamoto K, Lin Z, Kasugai S, Igarashi Y and Yamaguchi A: Canine oral mucosal fibroblasts differentiate into osteoblastic cells in response to BMP-2. Anat Rec (Hoboken). 295:1327–1335. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Rutherford RB, Moalli M, Franceschi RT, Wang D, Gu K and Krebsbach PH: Bone morphogenetic protein-transduced human fibroblasts convert to osteoblasts and form bone in vivo. Tissue Eng. 8:441–452. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Ducy P, Schinke T and Karsenty G: The osteoblast: A sophisticated fibroblast under central surveillance. Science. 289:1501–1504. 2000. View Article : Google Scholar : PubMed/NCBI

23 

Harada H, Tagashira S, Fujiwara M, Ogawa S, Katsumata T, Yamaguchi A, Komori T and Nakatsuka M: Cbfa1 isoforms exert functional differences in osteoblast differentiation. J Biol Chem. 274:6972–6978. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Banerjee C, Hiebert SW, Stein JL, Lian JB and Stein GS: An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteocalcin gene. Proc Natl Acad Sci USA. 93:4968–4973. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Kern B, Shen J, Starbuck M and Karsenty G: Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes. J Biol Chem. 276:7101–7107. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Lee MH, Javed A, Kim HJ, Shin HI, Gutierrez S, Choi JY, Rosen V, Stein JL, van Wijnen AJ, Stein GS, et al: Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor beta1 in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not sufficient for osteoblast differentiation. J Cell Biochem. 73:114–125. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Loiselle AE, Paul EM, Lewis GS and Donahue HJ: Osteoblast and osteocyte-specific loss of Connexin43 results in delayed bone formation and healing during murine fracture healing. J Orthop Res. 31:147–154. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Li S, Zhang H, Li S, Yang Y, Huo B and Zhang D: Connexin43 and ERK regulate tension-induced signal transduction in human periodontal ligament fibroblasts. J Orthop Res. 33:1008–1014. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Niger C, Lima F, Yoo DJ, Gupta RR, Buo AM, Hebert C and Stains JP: The transcriptional activity of osterix requires the recruitment of Sp1 to the osteocalcin proximal promoter. Bone. 49:683–692. 2011. View Article : Google Scholar : PubMed/NCBI

30 

Park MC, Park YB and Lee SK: Relationship of bone morphogenetic proteins to disease activity and radiographic damage in patients with ankylosing spondylitis. Scand J Rheumatol. 37:200–204. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Chen MH, Chen HA, Chen WS, Chen MH, Tsai CY and Chou CT: Upregulation of BMP-2 expression in peripheral blood mononuclear cells by proinflammatory cytokines and radiographic progression in ankylosing spondylitis. Mod Rheumatol. 25:913–918. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Li F, Fan C, Zeng B, Zhang C, Chai Y, Liu S and Ouyang Y: Celecoxib suppresses fibroblast proliferation and collagen expression by inhibiting ERK1/2 and SMAD2/3 phosphorylation. Mol Med Rep. 5:827–831. 2012.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhou YY, Huang RY, Lin JH, Xu YY, He XH and He YT: Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43. Exp Ther Med 12: 347-353, 2016.
APA
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., & He, Y. (2016). Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43. Experimental and Therapeutic Medicine, 12, 347-353. https://doi.org/10.3892/etm.2016.3292
MLA
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., He, Y."Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43". Experimental and Therapeutic Medicine 12.1 (2016): 347-353.
Chicago
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., He, Y."Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43". Experimental and Therapeutic Medicine 12, no. 1 (2016): 347-353. https://doi.org/10.3892/etm.2016.3292
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou YY, Huang RY, Lin JH, Xu YY, He XH and He YT: Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43. Exp Ther Med 12: 347-353, 2016.
APA
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., & He, Y. (2016). Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43. Experimental and Therapeutic Medicine, 12, 347-353. https://doi.org/10.3892/etm.2016.3292
MLA
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., He, Y."Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43". Experimental and Therapeutic Medicine 12.1 (2016): 347-353.
Chicago
Zhou, Y., Huang, R., Lin, J., Xu, Y., He, X., He, Y."Bushen‑Qiangdu‑Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43". Experimental and Therapeutic Medicine 12, no. 1 (2016): 347-353. https://doi.org/10.3892/etm.2016.3292
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team