Benefit of HSP90α intervention on ischemia-reperfusion injury of venous blood-congested flaps Corrigendum in /10.3892/etm.2016.3488

Hu,Xiao-Ying; Chen,Zhen-Yu; Zhang,Bin; Leng,Xiang-Feng; Fan,Xiao-Jian; Liu,Tao

doi:10.3892/etm.2016.3317

July-2016 Volume 12 Issue 1

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July-2016 Volume 12 Issue 1

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Article Open Access

Benefit of HSP90α intervention on ischemia-reperfusion injury of venous blood-congested flaps

Corrigendum in: /10.3892/etm.2016.3488

Authors:
- Xiao-Ying Hu
- Zhen-Yu Chen
- Bin Zhang
- Xiang-Feng Leng
- Xiao-Jian Fan
- Tao Liu
View Affiliations / Copyright

Affiliations: Department of Plastic and Cosmetic Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Breast Surgery, Qingdao Eighth People's Hospital, Qingdao, Shandong 266100, P.R. China, Department of Otolaryngology, Qingdao Eighth People's Hospital, Qingdao, Shandong 266100, P.R. China

Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 177-182
|
Published online on: May 9, 2016

https://doi.org/10.3892/etm.2016.3317
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Abstract

In order to decrease the incidence of flap necrosis after reconstructive surgeries, new approaches are required. In the present study, a model of venous congested flaps in rats was established to test the heat shock protein (HSP) 90α, ‘F-5’, protein as an intervention therapy to alleviate ischemia-reperfusion injury. A recombinant plasmid pET15b-F-5 carrying the HSP90α gene was constructed and the induced protein was purified from bacterial cell cultures. The rats in the study were divided into three different intervention groups: group A rats were treated with normal saline prior to flap establishment, group B rats were treated with HSP90α, ‘F-5’, protein prior to flap establishment, and group C rats were treated with the same ‘F-5’ protein after the surgical procedure. Additionally, the reperfusion time-points, ischemia for 6 or 8 h (5 rats each), were established in each group. After set periods of time, the flaps were observed for skin appearance, blood flow, survival rate and histological changes including neovascularization and re-epithelialization. The results showed that the flaps in the rats pre-treated with ‘F-5’ protein performed better than the flaps of rats in the other two groups: the blood flow was higher, flap survival rate was increased, inflammatory cell infiltration was decreased and angiogenesis increased, and new skin structure was better completed by the end of the experiment. The parameters examind were improved for all the groups when the ischemia time was 6 h instead of 8 h. In conclusion, HSP90α intervention prior to flap establishment was shown to be beneficial in the model of ischemia-reperfusion injury in venous-congested flaps.

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1	Park CJ and Seo YS: Heat shock proteins: a review of the molecular chaperones for plant immunity. Plant Pathol J. 31:323–333. 2015. View Article : Google Scholar : PubMed/NCBI
2	Woodley DT, Wysong A, DeClerck B, Chen M and Li W: Keratinocyte migration and a hypothetical new role for extracellular heat shock protein 90 alpha in orchestrating skin wound healing. Adv Wound Care (New Rochelle). 4:203–212. 2015. View Article : Google Scholar : PubMed/NCBI
3	Jayaprakash P, Dong H, Zou M, Bhatia A, O'Brien K, Chen M, Woodley DT and Li W: Hsp90α and Hsp90β together operate a hypoxia and nutrient paucity stress-response mechanism during wound healing. J Cell Sci. 128:1475–1480. 2015. View Article : Google Scholar : PubMed/NCBI
4	Wright L, Barril X, Dymock B, Sheridan L, Surgenor A, Beswick M, Drysdale M, Collier A, Massey A, Davies N, et al: Structure-activity relationships in purine-based inhibitor binding to HSP90 isoforms. Chem Biol. 11:775–785. 2004. View Article : Google Scholar : PubMed/NCBI
5	Imai Y, Sakurai M, Horinouchi T, Lee YS and Yamada A: Epithelial cells and adipose cells both have their own temporal profile in 72-kd heat-shock protein expression determining their tolerance for ischaemia. J Plast Reconstr Aesthet Surg. 59:230–238. 2006. View Article : Google Scholar : PubMed/NCBI
6	Babovic S, Angel MF, Im MJ, Ress AM and Manson PN: Effects of tissue expansion on secondary ischemic tolerance in experimental free flaps. Ann Plast Surg. 34:593–598. 1995. View Article : Google Scholar : PubMed/NCBI
7	Hauge EM, Balling E, Hartmund T and Hjortdal VE: Secondary ischemia caused by venous or arterial occlusion shows differential effects on myocutaneous island flap survival and muscle ATP levels. Plast Reconstr Surg. 99:825–833. 1997. View Article : Google Scholar : PubMed/NCBI
8	Petry JJ and Wortham KA: The anatomy of the epigastric flap in the experimental rat. Plast Reconstr Surg. 74:410–413. 1984. View Article : Google Scholar : PubMed/NCBI
9	Wang BH, Ye C, Stagg CA, Lin M, Fawcett T, VanderKolk CA and Udelsman R: Improved free musculocutaneous flap survival with induction of heat shock protein. Plast Reconstr Surg. 101:776–784. 1998. View Article : Google Scholar : PubMed/NCBI
10	Li W, Li Y, Guan S, Fan J, Cheng CF, Bright AM, Chinn C, Chen M and Woodley DT: Extracellular heat shock protein-90alpha: Linking hypoxia to skin cell motility and wound healing. EMBO J. 26:1221–1233. 2007. View Article : Google Scholar : PubMed/NCBI
11	Cheng CF, Sahu D, Tsen F, Zhao Z, Fan J, Kim R, Wang X, O'Brien K, Li Y, Kuang Y, et al: A fragment of secreted Hsp90α carries properties that enable it to accelerate effectively both acute and diabetic wound healing in mice. J Clin Invest. 121:4348–4361. 2011. View Article : Google Scholar : PubMed/NCBI
12	Harashina T, Sawada Y and Watanabe S: The relationship between venous occlusion time in island flaps and flap survivals. Plast Reconstr Surg. 60:92–95. 1977. View Article : Google Scholar : PubMed/NCBI
13	Zhang Y, Bai X, Wang Y, Li N, Li X, Han F, Su L and Hu D: Role for heat shock protein 90α in the proliferation and migration of HaCaT cells and in the deep second-degree burn wound healing in mice. PLoS One. 9:e1037232014. View Article : Google Scholar : PubMed/NCBI