Open Access

Osteoarthritis prevention and meniscus regeneration induced by transplantation of mesenchymal stem cell sheet in a rat meniscal defect model

  • Authors:
    • Yiying Qi
    • Guangnan Chen
    • Gang Feng
  • View Affiliations

  • Published online on: May 10, 2016     https://doi.org/10.3892/etm.2016.3325
  • Pages: 95-100
  • Copyright: © Qi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Transplantation of mesenchymal stem cells (MSCs) is a potential therapy for meniscus regeneration. However, when using single cell suspension injection, there is frequently a significant loss of cells, with only a small percentage of cells remaining at the target site. This issue may be solved with the use of MSC sheets. In the present study, we investigated whether the use of MSC sheets were able to regenerate the meniscus effectively in a rat meniscectomized model. The anterior half of the medial meniscus in 10 rats was excised and an MSC sheet was transplanted in the MSC sheet treatment group, while untreated rats served as the control. After 4 and 8 weeks, the knee joints were examined by gross and histological observation. Histological observation revealed that the anterior portion of meniscus was similar to the native tissue, showing typical fibrochondrocytes surrounded by richer extracellular matrix in the MSC sheet group. In addition, predominant collagen-rich matrix bridging the interface was observed and the neo‑meniscus integrated well with its host meniscus. Furthermore, degenerative changes of tibial plateau and femoral condyle occurred in the two groups. MSC sheet transplantation alleviated the degenerative changes efficiently. In conclusion, transplantation of MSC sheets may efficiently promote meniscus regeneration, as well as inhibit the progression of osteoarthritis in knee joints.
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July-2016
Volume 12 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Qi Y, Chen G and Feng G: Osteoarthritis prevention and meniscus regeneration induced by transplantation of mesenchymal stem cell sheet in a rat meniscal defect model. Exp Ther Med 12: 95-100, 2016
APA
Qi, Y., Chen, G., & Feng, G. (2016). Osteoarthritis prevention and meniscus regeneration induced by transplantation of mesenchymal stem cell sheet in a rat meniscal defect model. Experimental and Therapeutic Medicine, 12, 95-100. https://doi.org/10.3892/etm.2016.3325
MLA
Qi, Y., Chen, G., Feng, G."Osteoarthritis prevention and meniscus regeneration induced by transplantation of mesenchymal stem cell sheet in a rat meniscal defect model". Experimental and Therapeutic Medicine 12.1 (2016): 95-100.
Chicago
Qi, Y., Chen, G., Feng, G."Osteoarthritis prevention and meniscus regeneration induced by transplantation of mesenchymal stem cell sheet in a rat meniscal defect model". Experimental and Therapeutic Medicine 12, no. 1 (2016): 95-100. https://doi.org/10.3892/etm.2016.3325