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Article

Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma

  • Authors:
    • Qi Chen
    • Bin Cao
    • Ning Nan
    • Yu Wang
    • Xu Zhai
    • Youfang Li
    • Tie Chong
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 377-383
    |
    Published online on: May 11, 2016
       https://doi.org/10.3892/etm.2016.3335
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Abstract

The function of kinesin family member 18A (KIF18A) in human renal cell carcinoma (RCC) is unclear. The purpose of the current study was to determine the expression and prognostic significance of KIF18A in RCC. Specimens from 273 RCC patients undergoing nephrectomies were studied. Expression of KIF18A mRNA was examined by reverse transcription‑polymerase chain reaction (RT‑PCR) or quantitative PCR, and the expression of KIF18A protein was examined by immunohistochemistry and western blotting. The expression of KIF18A in clear‑cell RCC cell lines was decreased using small interfering RNA targeting KIF18A, and increased by transfection with KIF18A cDNA. The proliferative ability of RCC cells in vitro and in vivo was detected by WST‑1 assay and an animal xenograft model with BALB/c nude mice, respectively. The association between KIF18A expression and overall survival was calculated using Kaplan‑Meier analysis. The results showed that KIF18A expression was significantly increased in RCC tissues compared with normal kidney tissues. The level of KIF18A expression was significantly associated with tumor stage, histological grade, metastasis and tumor size. Moreover, KIF18A increased the proliferation of RCC cells in vitro and in vivo. KIF18A expression was upregulated in RCC and enhanced the proliferation of RCC cells. Therefore, it appears that KIF18A plays a key role in the carcinogenesis and progression of RCC, and is a novel candidate prognostic marker for RCC patients. Furthermore, silencing KIF18A expression may serve as a new therapeutic strategy against RCC.
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1 

Siegel R, Naishadham D and Jemal A: Cancer statistics, 2013. CA Cancer J Clin. 63:11–30. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Deng FM and Melamed J: Histologic variants of renal cell carcinoma: Does tumor type influence outcome? Urol Clin North Am. 39:119–132. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Yang L, Parkin DM, Ferlay J, Li L and Chen Y: Estimates of cancer incidence in China for 2000 and projections for 2005. Cancer Epidemiol Biomark Prev. 14:243–250. 2005.

4 

Athar U and Gentile TC: Treatment options for metastatic renal cell carcinoma: A review. Can J Urol. 15:3954–3966. 2008.PubMed/NCBI

5 

Janowitz T, Welsh SJ, Zaki K, Mulders P and Eisen T: Adjuvant therapy in renal cell carcinoma - past, present and future. Semin Oncol. 40:482–491. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Ljungberg B, Cowan NC, Hanbury DC, Hora M, Kuczyk MA, Merseburger AS, Patard JJ, Mulders PF and Sinescu IC: European Association of Urology Guideline Group: EAU guidelines on renal cell carcinoma: The 2010 update. Eur Urol. 58:398–406. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Bratslavsky G, Sanford T, Srinivasan R, Aprelikova O, Liu J, Quezado M, Merino M and Linehan WM: Differential genetic expression in large versus small clear cell renal cell carcinoma: Results from microarray analysis. J Cancer. 2:271–279. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Jordan MA and Wilson L: Microtubules as a target for anticancer drugs. Nat Rev Cancer. 4:253–265. 2004. View Article : Google Scholar : PubMed/NCBI

9 

Gardner MK, Odde KJ and Bloom K: Kinesin-8 molecular motors: Putting the brakes on chromosome oscillations. Trends Cell Biol. 18:307–310. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Du Y, English CA and Ohi R: The kinesin-8 Kif18A dampens microtubule plus-end dynamics. Curr Biol. 20:374–380. 2010. View Article : Google Scholar : PubMed/NCBI

11 

Weaver LN, Ems-McClung SC, Stout JR, LeBlanc C, Shaw SL, Gardner MK and Walczak CE: Kif18A uses a microtubule binding site in the tail for plus-end localization and spindle length regulation. Curr Biol. 21:1500–1506. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Mayr MI, Hümmer S, Bormann J, Grüner T, Adio S, Woehlke G and Mayer TU: The human kinesin Kif18A is a motile microtubule depolymerase essential for chromosome congression. Curr Biol. 17:488–498. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Hartwell LH and Kastan MB: Cell cycle control and cancer. Science. 266:1821–1828. 1994. View Article : Google Scholar : PubMed/NCBI

14 

Malumbres M and Barbacid M: Cell cycle, CDKs and cancer: A changing paradigm. Nat Rev Cancer. 9:153–166. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Mayr MI, Storch M, Howard J and Mayer TU: A non-motor microtubule binding site is essential for the high processivity and mitotic function of kinesin-8 Kif18A. PLoS One. 6:e274712011. View Article : Google Scholar : PubMed/NCBI

16 

Zhang C, Zhu C, Chen H, Li L, Guo L, Jiang W and Lu SH: Kif18A is involved in human breast carcinogenesis. Carcinogenesis. 31:1676–1684. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Nagahara M, Nishida N, Iwatsuki M, Ishimaru S, Mimori K, Tanaka F, Nakagawa T, Sato T, Sugihara K, Hoon DS and Mori M: Kinesin 18A expression: Clinical relevance to colorectal cancer progression. Int J Cancer. 129:2543–2552. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Tooker BC, Newman LS, Bowler RP, Karjalainen A, Oksa P, Vainio H, Pukkala E and Brandt-Rauf PW: Proteomic detection of cancer in asbestosis patients using SELDI-TOF discovered serum protein biomarkers. Biomarkers. 16:181–191. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Rucksaken R, Khoontawad J, Roytrakul S, Pinlaor P, Hiraku Y, Wongkham C, Pairojkul C, Boonmars T and Pinlaor S: Proteomic analysis to identify plasma orosomucoid 2 and kinesin 18A as potential biomarkers of cholangiocarcinoma. Cancer Biomark. 12:81–95. 2012.PubMed/NCBI

20 

Elmore JM, Kadesky KT, Koeneman KS and Sagalowsky AI: Reassessment of the 1997 TNM classification system for renal cell carcinoma. Cancer. 98:2329–2334. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Hong SK, Jeong CW, Park JH, Kim HS, Kwak C, Choe G, Kim HH and Lee SE: Application of simplified Fuhrman grading system in clear-cell renal cell carcinoma. BJU Int. 107:409–415. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Johnson GL, Bibby DF, Wong S, Agrawal SG and Bustin SA: A MIQE-compliant real-time PCR assay for Aspergillus detection. PLoS One. 7:e400222012. View Article : Google Scholar : PubMed/NCBI

23 

Grimholt RM, Urdal P, Klingenberg O and Piehler AP: Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR. BMC Hematol. 14:42014. View Article : Google Scholar : PubMed/NCBI

24 

Rath O and Kozielski F: Kinesins and cancer. Nat Rev Cancer. 12:527–539. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Wood KW, Cornwell WD and Jackson JR: Past and future of the mitotic spindle as an oncology target. Curr Opin Pharmacol. 1:370–377. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Huszar D, Theoclitou ME, Skolnik J and Herbst R: Kinesin motor proteins as targets for cancer therapy. Cancer Metastasis Rev. 28:197–208. 2009. View Article : Google Scholar : PubMed/NCBI

27 

Liao W, Huang G, Liao Y, Yang J, Chen Q, Xiao S, Jin J, He S and Wang C: High KIF18A expression correlates with unfavorable prognosis in primary hepatocellular carcinoma. Oncotarget. 5:10271–10279. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Zhu H, Xu W, Zhang H, Liu J, Xu H, Lu S, Dang S, Kuang Y, Jin X and Wang Z: Targeted deletion of Kif18a protects from colitis-associated colorectal (CAC) tumors in mice through impairing Akt phosphorylation. Biochem Biophys Res Commun. 438:97–102. 2013. View Article : Google Scholar : PubMed/NCBI

29 

De Wever V, Nasa I, Chamousset D, Lloyd D, Nimick M, Xu H, Trinkle-Mulcahy L and Moorhead GB: The human mitotic kinesin KIF18A binds protein phosphatase 1 (PP1) through a highly conserved docking motif. Biochem Biophys Res Commun. 453:432–437. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Zusev M and Benayahu D: The regulation of MS-KIF18A expression and cross talk with estrogen receptor. PLoS One. 4:e64072009. View Article : Google Scholar : PubMed/NCBI

31 

Zusev M and Benayahu D: New insights on cellular distribution, microtubule interactions and post-translational modifications of MS-KIF18A. J Cell Physiol. 217:618–625. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen Q, Cao B, Nan N, Wang Y, Zhai X, Li Y and Chong T: Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma. Exp Ther Med 12: 377-383, 2016.
APA
Chen, Q., Cao, B., Nan, N., Wang, Y., Zhai, X., Li, Y., & Chong, T. (2016). Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma. Experimental and Therapeutic Medicine, 12, 377-383. https://doi.org/10.3892/etm.2016.3335
MLA
Chen, Q., Cao, B., Nan, N., Wang, Y., Zhai, X., Li, Y., Chong, T."Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma". Experimental and Therapeutic Medicine 12.1 (2016): 377-383.
Chicago
Chen, Q., Cao, B., Nan, N., Wang, Y., Zhai, X., Li, Y., Chong, T."Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma". Experimental and Therapeutic Medicine 12, no. 1 (2016): 377-383. https://doi.org/10.3892/etm.2016.3335
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Q, Cao B, Nan N, Wang Y, Zhai X, Li Y and Chong T: Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma. Exp Ther Med 12: 377-383, 2016.
APA
Chen, Q., Cao, B., Nan, N., Wang, Y., Zhai, X., Li, Y., & Chong, T. (2016). Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma. Experimental and Therapeutic Medicine, 12, 377-383. https://doi.org/10.3892/etm.2016.3335
MLA
Chen, Q., Cao, B., Nan, N., Wang, Y., Zhai, X., Li, Y., Chong, T."Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma". Experimental and Therapeutic Medicine 12.1 (2016): 377-383.
Chicago
Chen, Q., Cao, B., Nan, N., Wang, Y., Zhai, X., Li, Y., Chong, T."Elevated expression of KIF18A enhances cell proliferation and predicts poor survival in human clear cell renal carcinoma". Experimental and Therapeutic Medicine 12, no. 1 (2016): 377-383. https://doi.org/10.3892/etm.2016.3335
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