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Establishment of a reversible model of prehepatic portal hypertension in rats

  • Authors:
    • Xin Zhao
    • Jian Dou
    • Qing‑Jun Gao
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, The Third Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 939-944
    |
    Published online on: May 30, 2016
       https://doi.org/10.3892/etm.2016.3405
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Abstract

The aim of the present study was to improve upon the traditional model of pre‑hepatic portal hypertension in rats, and simulate the anhepatic phase of orthotopic liver transplantation without veno‑venous bypass. A reversible model of portal hypertension was induced by portal vein ligation, with a label ring ligated along the portal vein. A total of 135 male Wistar rats were divided into three groups: i) Normal control (NC) group; ii) portal hypertensive control (PHTC) group; and iii) reperfusion (R) group. In the R group, rats with portal hypertension underwent simultaneous clamping of the portal triad and retrohepatic vena cava for 1 h, followed by removal of the clamps to enable blood reperfusion. Portal venography and portal vein pressure were recorded during the surgery. Arterial oxygen pressure (PaO2), and alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBil) levels were determined, and pathological changes of the liver were investigated by immunohistochemical staining. The results demonstrated that, 3 weeks after portal vein ligation, the vein area and the free portal pressures in the PHTC group were significantly increased compared with those in the NC group. The serum ALT and AST levels in the R group at different time points were significantly elevated compared with those in the PHTC group, and reached their maximal levels at 24 h after reperfusion. Furthermore, the PaO2 at 24 h after reperfusion was significantly decreased. In conclusion, the reversible model of pre‑hepatic portal hypertension in rats was successfully established using the introduction of a label ring. This model may be useful for basic research focusing on the anhepatic phase of orthotopic liver transplantation without veno‑venous bypass.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Dou J and Gao QJ: Establishment of a reversible model of prehepatic portal hypertension in rats. Exp Ther Med 12: 939-944, 2016.
APA
Zhao, X., Dou, J., & Gao, Q. (2016). Establishment of a reversible model of prehepatic portal hypertension in rats. Experimental and Therapeutic Medicine, 12, 939-944. https://doi.org/10.3892/etm.2016.3405
MLA
Zhao, X., Dou, J., Gao, Q."Establishment of a reversible model of prehepatic portal hypertension in rats". Experimental and Therapeutic Medicine 12.2 (2016): 939-944.
Chicago
Zhao, X., Dou, J., Gao, Q."Establishment of a reversible model of prehepatic portal hypertension in rats". Experimental and Therapeutic Medicine 12, no. 2 (2016): 939-944. https://doi.org/10.3892/etm.2016.3405
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Dou J and Gao QJ: Establishment of a reversible model of prehepatic portal hypertension in rats. Exp Ther Med 12: 939-944, 2016.
APA
Zhao, X., Dou, J., & Gao, Q. (2016). Establishment of a reversible model of prehepatic portal hypertension in rats. Experimental and Therapeutic Medicine, 12, 939-944. https://doi.org/10.3892/etm.2016.3405
MLA
Zhao, X., Dou, J., Gao, Q."Establishment of a reversible model of prehepatic portal hypertension in rats". Experimental and Therapeutic Medicine 12.2 (2016): 939-944.
Chicago
Zhao, X., Dou, J., Gao, Q."Establishment of a reversible model of prehepatic portal hypertension in rats". Experimental and Therapeutic Medicine 12, no. 2 (2016): 939-944. https://doi.org/10.3892/etm.2016.3405
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