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E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143

  • Authors:
    • Jibin Li
    • Xinling Wang
    • Yanshang Zhang
    • Yan Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Hebei General Hospital, Shijiazhuang, Hebei 050051, P.R. China, Department of Orthopedics, Gongli Hospital of Pudong New Area, Shanghai 200135, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 676-682
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    Published online on: June 6, 2016
       https://doi.org/10.3892/etm.2016.3429
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Abstract

Cervical cancer is one of the most common gynecological cancers worldwide. Aberrant expression of E3 ubiquitin ligase isolated by differential display (EDD) has been detected in various types of tumor and has been demonstrated to have an important role in carcinogenesis, tumor growth and drug resistance. However, the role of EDD in cervical cancer and its underlying molecular mechanisms remains unknown. The present study aimed to investigate the role of EDD in the tumorigenicity of cervical cancer. EDD expression levels were measured using reverse transcription‑quantitative polymerase chain reaction and western blotting in SiHa, HeLa, CaSki, c‑41 and c‑33A cervical cancer cell lines and cervical cancer tissue specimens. A functional study was performed using cell proliferation, colony formation, cell apoptosis assays in vitro and tumor growth assays in vivo with EDD either overexpressed or silenced. In the present study, EDD expression levels were significantly upregulated in cervical cancer cell lines and tissue samples. EDD knockdown significantly inhibited colony formation, cell proliferation and tumor growth and accelerated cell apoptosis in the cervical cancer cell lines and tissue samples. Furthermore, microRNA (miR)‑143 expression levels were low in cervical cancer tissue samples and were negatively correlated with EDD expression. miR‑143 silencing eliminated the effect of EDD on cell proliferation, colony formation and cell apoptosis in the cervical cancer cells, which suggested that miR‑143 is critical for EDD‑mediated regulation of cervical cancer cell growth. The results of the present study indicated that EDD may promote cervical cancer growth in vivo and in vitro by targeting miR‑143. In conclusion, EDD may have an oncogenic role in cervical cancer and may serve as a potential therapeutic target for the treatment of patients with cervical cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Wang X, Zhang Y and Zhang Y: E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143. Exp Ther Med 12: 676-682, 2016.
APA
Li, J., Wang, X., Zhang, Y., & Zhang, Y. (2016). E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143. Experimental and Therapeutic Medicine, 12, 676-682. https://doi.org/10.3892/etm.2016.3429
MLA
Li, J., Wang, X., Zhang, Y., Zhang, Y."E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143". Experimental and Therapeutic Medicine 12.2 (2016): 676-682.
Chicago
Li, J., Wang, X., Zhang, Y., Zhang, Y."E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143". Experimental and Therapeutic Medicine 12, no. 2 (2016): 676-682. https://doi.org/10.3892/etm.2016.3429
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Spandidos Publications style
Li J, Wang X, Zhang Y and Zhang Y: E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143. Exp Ther Med 12: 676-682, 2016.
APA
Li, J., Wang, X., Zhang, Y., & Zhang, Y. (2016). E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143. Experimental and Therapeutic Medicine, 12, 676-682. https://doi.org/10.3892/etm.2016.3429
MLA
Li, J., Wang, X., Zhang, Y., Zhang, Y."E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143". Experimental and Therapeutic Medicine 12.2 (2016): 676-682.
Chicago
Li, J., Wang, X., Zhang, Y., Zhang, Y."E3 ubiquitin ligase isolated by differential display regulates cervical cancer growth in vitro and in vivo via microRNA-143". Experimental and Therapeutic Medicine 12, no. 2 (2016): 676-682. https://doi.org/10.3892/etm.2016.3429
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