Effects of testosterone on the expression levels of AMH, VEGF and HIF‑1α in mouse granulosa cells

Zhang,Ying; Wang,Shan‑Feng; Zheng,Jin‑Dan; Zhao,Chun‑Bo; Zhang,Yi‑Nan; Liu,Li‑Li; Huang,Jian‑Hua

doi:10.3892/etm.2016.3436

August-2016 Volume 12 Issue 2

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August-2016 Volume 12 Issue 2

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Effects of testosterone on the expression levels of AMH, VEGF and HIF‑1α in mouse granulosa cells

Authors:
- Ying Zhang
- Shan‑Feng Wang
- Jin‑Dan Zheng
- Chun‑Bo Zhao
- Yi‑Nan Zhang
- Li‑Li Liu
- Jian‑Hua Huang
View Affiliations / Copyright

Affiliations: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China, Department of Key Laboratory of Tissue Engineering, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121000, P.R. China

Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 883-888
|
Published online on: June 8, 2016

https://doi.org/10.3892/etm.2016.3436
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Abstract

The present study aimed to investigate the effects of testosterone on mouse granulosa cell morphology, and the expression levels of anti‑Müllerian hormone (AMH), vascular endothelial growth factor (VEGF) and hypoxia‑inducible factor‑1α (HIF‑1α). Mouse granulosa cells were isolated and identified, and their morphology was examined using hematoxylin and eosin, F‑actin, and follicle‑stimulating hormone receptor staining. The mRNA expression levels of AMH, VEGF and HIF‑1α were examined using reverse transcription‑quantitative polymerase chain reaction, and their protein secretion levels were investigated using enzyme‑linked immunosorbent assays. Testosterone treatment did not affect granulosa cell morphology; however, it significantly increased the mRNA expression levels of AMH and VEGF, and the protein secretion levels of AMH, VEGF and HIF‑1α. These results suggested that testosterone was able to regulate the functions of granulosa cells by upregulating the expression levels of AMH, VEGF and HIF‑1α.

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1	Trivax B and Azziz R: Diagnosis of polycystic ovary syndrome. Clin Obstet Gynecol. 50:168–177. 2007. View Article : Google Scholar : PubMed/NCBI
2	Azziz R, Carmina E, Dewailly D, Diamanti-Kandarakis E, Escobar-Morreale HF, Futterweit W, Janssen OE, Legro RS, Norman RJ, Taylor AE and Witchel SF: Androgen Excess Society: Positions statement: Criteria for defining polycystic ovary syndrome as a predominantly hyperandrogenic syndrome: An Androgen Excess Society guideline. J Clin Endocrinol Metab. 91:4237–4245. 2006. View Article : Google Scholar : PubMed/NCBI
3	Barber TM, Wass JA, McCarthy MI and Franks S: Metabolic characteristics of women with polycystic ovaries and oligo-amenorrhoea but normal androgen levels: Implications for the management of polycystic ovary syndrome. Clin Endocrinol (Oxf). 66:513–517. 2007.PubMed/NCBI
4	Dokras A, Clifton S, Futterweit W and Wild R: Increased risk forabnormal depression scores in women with polycystic ovary syndrome: A systematic review and meta-analysis. Obstet Gynecol. 117:145–152. 2011. View Article : Google Scholar : PubMed/NCBI
5	Solomon CG: The epidemiology of polycystic ovary syndrome. Prevalence and associated disease risks. Endocrinol Metab Clin North Am. 28:247–263. 1999. View Article : Google Scholar : PubMed/NCBI
6	Schneider JG, Tompkins C, Blumenthal RS and Mora S: The metabolic syndrome in women. Cardiol Rev. 14:286–291. 2006. View Article : Google Scholar : PubMed/NCBI
7	Duncan WC, van den Driesche S and Fraser HM: Inhibition of vascular endothelial growth factor in the primate ovary up-regulates hypoxia-inducible factor-1alpha in the follicle and corpus luteum. Endocrinology. 149:3313–3320. 2008. View Article : Google Scholar : PubMed/NCBI
8	Keith B, Johnson RS and Simon MC: HIF1α and HIF2α: Sibling rivalry in hypoxic tumour growth and progression. Nat Rev Cancer. 12:9–22. 2011.PubMed/NCBI
9	van Disseldorp J, Faddy MJ, Themmen AP, de Jong FH, Peeters PH, van der Schouw YT and Broekmans FJ: Relationship of serum antimüllerian hormone concentration to age at menopause. J Clin Endocrinol Metab. 93:2129–2134. 2008. View Article : Google Scholar : PubMed/NCBI
10	Freeman EW, Gracia CR, Sammel MD, Lin H, Lim LC and Strauss JF III: Association of anti-mullerian hormone levels with obesity in late reproductive-age women. Fertil Steril. 87:101–106. 2007. View Article : Google Scholar : PubMed/NCBI
11	Ferrara N, Gerber HP and LeCouter J: The biology of VEGF and its receptors. Nat Med. 9:669–676. 2003. View Article : Google Scholar : PubMed/NCBI
12	Wang HX, Li TY and Kidder GM: WNT2 regulates DNA synthesis in mouse granulosa cells through beta-catenin. Biol Reprod. 82:865–875. 2010. View Article : Google Scholar : PubMed/NCBI
13	Vendola KA, Zhou J, Adesanya OO, Weil SJ and Bondy CA: Androgens stimulate early stages of follicular growth in the primate ovary. J Clin Invest. 101:2622–2629. 1998. View Article : Google Scholar : PubMed/NCBI
14	Weil SJ, Vendola K, Zhou J, Adesanya OO, Wang J, Okafor J and Bondy CA: Androgen receptor gene expression in the primate ovary: Cellular localization, regulation, and functional correlations. J Clin Endocrinol Metab. 83:2479–2485. 1998. View Article : Google Scholar : PubMed/NCBI
15	Duda M: The influence of FSH: LH and testosterone on steroidsecretion by two subpopulations of porcine granulosa cells. J Physiol Pharmacol. 48:89–96. 1997.PubMed/NCBI
16	Seifer DB and Maclaughlin DT: Mullerian inhibiting Substance is an ovarian growth factor of emerging clinical significance. Fertil Steril. 88:539–546. 2007. View Article : Google Scholar : PubMed/NCBI
17	Tal R and Seifer DB: Potential mechanisms for racial and ethnic differences in antimüllerian hormone and ovarian reserve. Int J Endocrinol. 2013:8189122013. View Article : Google Scholar : PubMed/NCBI
18	Veiga-Lopez A, Ye W and Padmanabhan V: Developmental programming: Prenatal testosterone excess disrupts anti-Müllerian hormone expression in preantral and antral follicles. Fertil Steril. 97:748–756. 2012. View Article : Google Scholar : PubMed/NCBI
19	Fraser HM and Duncan WC: Vascular morphogenesis in the primate ovary. Angiogenesis. 8:101–116. 2005. View Article : Google Scholar : PubMed/NCBI
20	Kisliouk T, Levy N, Hurwitz A and Meidan R: Presence and regulation of endocrine gland vascular endothelial growth factor/prokineticin-1 and its receptors in ovarian cells. J Clin Endocrinol Metab. 88:3700–3707. 2003. View Article : Google Scholar : PubMed/NCBI
21	Grzesiak M, Williams L and Luck MR: Testosterone influences water transport in porcine granulosa cells. Reprod Domest Anim. 48:e52–e54. 2013. View Article : Google Scholar : PubMed/NCBI
22	Vanderhyden BC and Macdonald EA: Mouse oocytes regulate granulosa cell steroidogenesis throughout follicular development. Biol Reprod. 59:1296–1301. 1998. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effects of testosterone on the expression levels of AMH, VEGF and HIF‑1α in mouse granulosa cells

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