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Article

Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region

  • Authors:
    • Majid Zarrin
    • Maryam Erfaninejad
  • View Affiliations / Copyright

    Affiliations: Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357‑15794, Iran, Department of Medical Mycology, Medical School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Khuzestan 61357‑15794, Iran
  • Pages: 1628-1632
    |
    Published online on: June 24, 2016
       https://doi.org/10.3892/etm.2016.3479
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Abstract

Aspergillus flavus is the second most common disease-causing species of Aspergillus in humans. The fungus is frequently associated with life‑threatening infections in immunocompromised hosts. The primary aim of the present study was to analyze the genetic variability among different isolates of A. flavus using polymerase chain reaction (PCR)‑based restriction fragment length polymorphism (RFLP). A total of 62 A. flavus isolates were tested in the study. Molecular variability was searched for by analysis of the PCR amplification of the internal transcribed spacer (ITS) regions of ribosomal DNA using restriction enzymes. PCR using primers for ITS1 and ITS4 resulted in a product of ~600 bp. Amplicons were subjected to digestion with restriction endonucleases EcoRI, HaeIII and TaqI. Digestion of the PCR products using these restriction enzymes produced different patterns of fragments among the isolates, with different sizes and numbers of fragments, revealing genetic variability. In conclusion, ITS‑RFLP is a useful molecular tool in screening for nucleotide polymorphisms among A. flavus isolates.
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1 

Krishnan S, Manavathu EK and Chandrasekar PH: Aspergillus flavus: An emerging non-fumigatus Aspergillus species of significance. Mycoses. 52:206–222. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Pasqualotto AC: Differences in pathogenicity and clinical syndromes due to Aspergillus fumigatus and Aspergillus flavus. Med Mycol. 47(Suppl 1): S261–S270. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Ehrlich KC and Mack BM: Comparison of Expression of Secondary Metabolite Biosynthesis Cluster Genes in Aspergillus flavus, A. parasiticus, and A. oryzae. Toxins (Basel). 6:1916–1928. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Karthikeyan M, Sandosskumar R, Mathiyazhagan S, Mohankumar M, Valluvaparidasan V, Kumar S and Velazhahan R: Genetic variability and aflatoxigenic potential of Aspergillus flavus isolates from maize. Arch Phytopathol Plant Protect. 42:83–91. 2009. View Article : Google Scholar

5 

Zhang ZG, Zhang JY, Zheng XB, Yang YW and Ko WH: Molecular distinctions between Phytophthora capsici and P. tropicalis based on ITS sequences of ribosomal DNA. J Phytopathol. 152:358–364. 2004. View Article : Google Scholar

6 

Khoodoo MHR and Jaufeerally-Fakim Y: RAPD-PCR fingerprinting and southern analysis of Xanthomonas axonopodis pv. Dieffenbachiae strains isolated from different aroid hosts and locations. Plant Dis. 88:980–988. 2004. View Article : Google Scholar

7 

Henry T, Iwen PC and Hinrichs SH: Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J Clin Microbiol. 38:1510–1515. 2000.PubMed/NCBI

8 

Gomes EA, Maria Kasuya CM, de Barros EG, Borges AC and Araújo EF: Polymorphism in the internal transcribed spacer (ITS) of the ribosomal DNA of 26 isolates of ectomycorrhizal fungi. Genet Mol Biol. 25:477–483. 2002. View Article : Google Scholar

9 

Krimitzas A, Pyrri I, Kouvelis VN, Kapsanaki-Gotsi E and Typas MA: A phylogenetic analysis of Greek isolates of Aspergillus species based on morphology and nuclear and mitochondrial gene sequences. Biomed Res Int. 2013:2603952013. View Article : Google Scholar : PubMed/NCBI

10 

Criseo G, Bagnara A and Bisignano G: Differentiation of aflatoxin producing and non-producing strains of Aspergillus flavus group. Lett Appl Microbiol. 33:291–295. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Chen RS, Tsay JG, Huang YF and Chiou RY: Polymerase chain reaction-mediated characterization of molds belonging to the Aspergillus flavus group and detection of Aspergillus parasiticus in peanut kernels by multiplex polymerase chain reaction. J Food Prot. 65:840–844. 2002.PubMed/NCBI

12 

Toju H, Tanabe AS, Yamamoto S and Sato H: High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples. PLoS One. 7:e408632012. View Article : Google Scholar : PubMed/NCBI

13 

Diguta CF, Vincent B, Guilloux-Benatier M, Alexandre H and Rousseaux S: PCR ITS-RFLP: A useful method for identifying filamentous fungi isolates on grapes. Food Microbiol. 28:1145–1154. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Pereira F, Carneiro J and Amorim A: Identification of species with DNA-based technology: Current progress and challenges. Recent Pat DNA Gene Seq. 2:187–199. 2008. View Article : Google Scholar : PubMed/NCBI

15 

Huang A, Li JW, Shen ZQ, Wang XW and Jin M: High-throughput identification of clinical pathogenic fungi by hybridization to an oligonucleotide microarray. J Clin Microbiol. 44:3299–3305. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Nicholson P, Simpson DR, Weston G, Rezanoor HN, Lees AK, Parry DW and Joyce D: Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays. Physiol Mol Plant Pathol. 53:17–37. 1998. View Article : Google Scholar

17 

O'Donnell K, Cigelnik E and Nirenberg HI: Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia. 90:465–493. 1998. View Article : Google Scholar

18 

Paterson RRM: Identification and quantification of mycotoxigenic fungi by PCR. Process Biochem. 71:1467–1474. 2006. View Article : Google Scholar

19 

Ferrer C, Colom F, Frasés S, Mulet E, Abad JL and Alió JL: Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J Clin Microbiol. 39:2873–2879. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Mirete S, Patiño B, Vázquez C, Jiménez M, Hinojo MJ, Soldevilla C and González-Jaén MT: Fumonisin production by Gibberella fujikuroi strains from Pinus species. Int J Food Microbiol. 89:213–221. 2003. View Article : Google Scholar : PubMed/NCBI

21 

González-Salgado A, Patño B, Vázquez C and González-Jaén MT: Discrimination of Aspergillus niger and other Aspergillus species belonging to section Nigri by PCR assays. FEMS Microbiol Lett. 245:353–361. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Kizis D, Natskoulis P, Nychas GJ and Panagou EZ: Biodiversity and ITS-RFLP characterisation of Aspergillus section Nigri isolates in grapes from four traditional grape-producing areas in Greece. PLoS One. 9:e939232014. View Article : Google Scholar : PubMed/NCBI

23 

Dubey SC, Tripathi A and Singh SR: ITS-RFLP fingerprinting and molecular marker for detection of Fusarium oxysporum f.sp. ciceris. Folia Microbiol (Praha). 55:629–634. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Mohankumar M, Vijayasamundeeswari A, Karthikeyan M, Mathiyazhagan S, Paranidharan V and Velazhahan R: Analysis of molecular variability among isolates of Aspergillus flavus by PCR-RFLP of the its regions of rDNA. J Plant Prot Res. 50:446–451. 2010. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Zarrin M and Erfaninejad M: Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region. Exp Ther Med 12: 1628-1632, 2016.
APA
Zarrin, M., & Erfaninejad, M. (2016). Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region. Experimental and Therapeutic Medicine, 12, 1628-1632. https://doi.org/10.3892/etm.2016.3479
MLA
Zarrin, M., Erfaninejad, M."Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region". Experimental and Therapeutic Medicine 12.3 (2016): 1628-1632.
Chicago
Zarrin, M., Erfaninejad, M."Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1628-1632. https://doi.org/10.3892/etm.2016.3479
Copy and paste a formatted citation
x
Spandidos Publications style
Zarrin M and Erfaninejad M: Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region. Exp Ther Med 12: 1628-1632, 2016.
APA
Zarrin, M., & Erfaninejad, M. (2016). Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region. Experimental and Therapeutic Medicine, 12, 1628-1632. https://doi.org/10.3892/etm.2016.3479
MLA
Zarrin, M., Erfaninejad, M."Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region". Experimental and Therapeutic Medicine 12.3 (2016): 1628-1632.
Chicago
Zarrin, M., Erfaninejad, M."Molecular variation analysis of Aspergillus flavus using polymerase chain reaction-restriction fragment length polymorphism of the internal transcribed spacer rDNA region". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1628-1632. https://doi.org/10.3892/etm.2016.3479
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