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Article

Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep

  • Authors:
    • Mingxing Zhu
    • Fu Gao
    • Zihua Li
    • Xiuqing Wang
    • Hao Wang
    • Zhisheng Wang
    • Jiaqing Zhao
    • Wei Zhao
  • View Affiliations / Copyright

    Affiliations: Center of Scientific Technology, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Institute of Clinical Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Key Laboratory of Hydatid Disease, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Experimental Animal Center, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
  • Pages: 1585-1590
    |
    Published online on: July 5, 2016
       https://doi.org/10.3892/etm.2016.3501
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Abstract

The aims of the present study were to investigate the immunoprotection of recombinant Echinococcus granulosus myophilin (rEg.myophilin) against the establishment of a challenge oral infection with E. granulosus eggs, as well as to determine the mechanisms underlying this protection. Sheep were subcutaneously immunized two times with rEg.myophilin, followed by the challenge with E. granulosus eggs orally. The animals were sacrificed 44 weeks after infection and the immunoglobulin (Ig) and cytokine levels were analyzed using ELISA. The results identified significant changes in several indexes of animal immune response subsequent to immunization with rEg.myophilin. These changes included reduced number of formed cysts, as well as elevated levels of IgG, IgA and cytokines. The present data suggest that immunization with rEg.myophilin in sheep can successfully reduce the formation of cysts caused by challenge E. granulosus infection and stimulate immune response, suggesting that rEg.myophilin a has potential value as a candidate vaccine against E. granulosus.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu M, Gao F, Li Z, Wang X, Wang H, Wang Z, Zhao J and Zhao W: Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep. Exp Ther Med 12: 1585-1590, 2016.
APA
Zhu, M., Gao, F., Li, Z., Wang, X., Wang, H., Wang, Z. ... Zhao, W. (2016). Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep. Experimental and Therapeutic Medicine, 12, 1585-1590. https://doi.org/10.3892/etm.2016.3501
MLA
Zhu, M., Gao, F., Li, Z., Wang, X., Wang, H., Wang, Z., Zhao, J., Zhao, W."Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep". Experimental and Therapeutic Medicine 12.3 (2016): 1585-1590.
Chicago
Zhu, M., Gao, F., Li, Z., Wang, X., Wang, H., Wang, Z., Zhao, J., Zhao, W."Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1585-1590. https://doi.org/10.3892/etm.2016.3501
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu M, Gao F, Li Z, Wang X, Wang H, Wang Z, Zhao J and Zhao W: Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep. Exp Ther Med 12: 1585-1590, 2016.
APA
Zhu, M., Gao, F., Li, Z., Wang, X., Wang, H., Wang, Z. ... Zhao, W. (2016). Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep. Experimental and Therapeutic Medicine, 12, 1585-1590. https://doi.org/10.3892/etm.2016.3501
MLA
Zhu, M., Gao, F., Li, Z., Wang, X., Wang, H., Wang, Z., Zhao, J., Zhao, W."Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep". Experimental and Therapeutic Medicine 12.3 (2016): 1585-1590.
Chicago
Zhu, M., Gao, F., Li, Z., Wang, X., Wang, H., Wang, Z., Zhao, J., Zhao, W."Immunoprotection of recombinant Eg.myophilin against echinococcus granulosus infection in sheep". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1585-1590. https://doi.org/10.3892/etm.2016.3501
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