miR‑155 functions downstream of angiotensin II receptor subtype 1 and calcineurin to regulate cardiac hypertrophy

  • Authors:
    • Yong Yang
    • Yong Zhou
    • Zheng Cao
    • Xin Zhu Tong
    • Hua Qiang Xie
    • Tao Luo
    • Xian Ping Hua
    • Han Qin Wang
  • View Affiliations

  • Published online on: July 5, 2016     https://doi.org/10.3892/etm.2016.3506
  • Pages: 1556-1562
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Abstract

Cardiac hypertrophy is characterized by maladaptive tissue remodeling that may lead to heart failure or sudden death. MicroRNAs (miRs) are negative regulators of angiotensin II and the angiotensin II receptor subtype 1 (AGTR1), which are two components involved in cardiac hypertrophy. In the present study, the interaction between angiotensin II receptor subtype 1 (AGTR1) signaling and miR‑155 was investigated. Rat H9C2 (2‑1) cardiomyocytes were transfected with miR‑155 analogues or inhibitors, then stimulated with angiotensin II to induce cardiac hypertrophy. miR‑155 expression was revealed to be altered following transfection with chemically‑modified miR‑155 analogues and inhibitors in rat cardiomyocytes. In cell cardiac hypertrophy models, the cell surface area, AGTR1, atrial natriuretic peptide and myosin heavy chain‑β mRNA expression levels were revealed to be lower in cells stimulated with miR‑155 analogue‑transfected cells treated with angiotensin II compared with cells stimulated with angiotensin alone (P<0.05), as determined using reverse transcription‑polymerase chain reaction (PCR), quantitative PCR and western blot analyses. Furthermore, calcineurin mRNA and protein, intracellular free calcium and nuclear factor of activated T‑cells‑4 proteins were downregulated in miR‑155 analogue‑transfected cells treated with angiotensin II, as compared with cells stimulated with angiotensin II alone (P<0.05). In conclusion, the current study indicates that miR‑155 may improve cardiac hypertrophy by downregulating AGTR1 and suppressing the calcium signaling pathways activated by AGTR1.
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September-2016
Volume 12 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Yang Y, Zhou Y, Cao Z, Tong XZ, Xie HQ, Luo T, Hua XP and Wang HQ: miR‑155 functions downstream of angiotensin II receptor subtype 1 and calcineurin to regulate cardiac hypertrophy. Exp Ther Med 12: 1556-1562, 2016
APA
Yang, Y., Zhou, Y., Cao, Z., Tong, X.Z., Xie, H.Q., Luo, T. ... Wang, H.Q. (2016). miR‑155 functions downstream of angiotensin II receptor subtype 1 and calcineurin to regulate cardiac hypertrophy. Experimental and Therapeutic Medicine, 12, 1556-1562. https://doi.org/10.3892/etm.2016.3506
MLA
Yang, Y., Zhou, Y., Cao, Z., Tong, X. Z., Xie, H. Q., Luo, T., Hua, X. P., Wang, H. Q."miR‑155 functions downstream of angiotensin II receptor subtype 1 and calcineurin to regulate cardiac hypertrophy". Experimental and Therapeutic Medicine 12.3 (2016): 1556-1562.
Chicago
Yang, Y., Zhou, Y., Cao, Z., Tong, X. Z., Xie, H. Q., Luo, T., Hua, X. P., Wang, H. Q."miR‑155 functions downstream of angiotensin II receptor subtype 1 and calcineurin to regulate cardiac hypertrophy". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1556-1562. https://doi.org/10.3892/etm.2016.3506