Astragaloside IV protects new born rats from anesthesia-induced apoptosis in the developing brain

Introduction

Anesthetics may damage the brain during the synaptogeneic phase of neurodevelopment due to their property at the N-methul-D-aspartic acid and γ-aminoburyric acid A receptors (1–3). The peak vulnerability window to the neurotoxicity is during synaptogenesis phase, also called brain growth spurt period (4,5). Such damage may affect the development of the neural network, and the effect may persist to adult age for the behavior and recognition ability (6,7). These findings raised the concerns about the neurotoxicity of general anesthetics, particularly the drugs used in pediatric anesthesia, where the human fetal brain may be damaged during the procedure (8).

Neuron apoptosis is the primary reason for the neurotoxicity, and the mechanisms of anesthesia-induced apoptosis have been well studied (5,9). Activation of proapoptotic proteins such as Bax, may lead to mitochondrial membrane breakage and activation of caspases, which execute cell apoptosis (10). On the other hand, generation of free radicals such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) leads to lipid peroxidation, which could cause brain damage. Such free radicals and damages may also elicit inflammatory responses, which further amplify the apoptotic response (11). By contrast, antioxidant enzymes such as superoxide dismutase (SOD), can scavenge excessive free radicals and alleviate their detrimental effect (12).

Astragaloside IV (AS IV) is a saponin purified from a traditional Chinese herbal medicine component Astragalus membraneaceus (Fisch.) Bunge (13,14). AS IV has exhibited antioxidant activity and anti-apoptosis function in various types of cells and tissues (15–19). For example, it ameliorates renal fibrosis by inhibiting mitogen-activated protein kinase (MAPK) activation and attenuating unilateral ureteral obstruction and transforming growth factor-β-induced renal tubular cell apoptosis (19,20). By contrast, AS IV is able to protect neuron cells from 1-methyl-4-phenylpyridnium ion-induced neurotoxicity by preventing ROS production and inhibition of the Bax-mediated proapoptotic pathway (21). AS IV also reduced amyloid-β-1-42-induced neurotoxicity by inhibiting the mitochrondrial permeability transition pore opening (22). However, its role in anesthesia-induced neuroapoptosis and associated neurotoxicity has not been investigated.

To determine whether AS IV is able to protect developing neurons from anesthesia-induced apoptosis, we investigated the effect of AS IV pre-treatment on isoflurane-induced neuron apoptosis in hippocampus tissues of new born rats. The levels of oxidative stress-related enzyme activities in hippocampus tissues and serum were quantified, and serum levels of proinflammatory cytokines were determined by ELISA. Protein expression levels of nuclear factor (NF)-κB, caspase-3, B-cell lymphoma 2 (Bcl-2), phosphorylated (p)-c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and protein kinase B (Akt) were detected by western blot assay. The results suggested that AS IV had a protective effect in preventing isoflurane-induced neurodegeneration, and indicated a potential mechanism underlying such a protective effect. Overall, the present study provides novel experimental evidence regarding the role of AS IV as a potential means for reducing anesthesia-associated neurotoxicity.

Materials and methods

Animals and reagents

A total of 20 male Sprague-Dawley rats at five days postpartum (P5) with body weight of 18–25 g were obtained from the animal experiment facility of the Shanghai Institute of Brain Functional Genomics Institute (Eastern China Normal University, Shanghai, China). All animal experiments were approved by the Institutional Animal Care and Use Committee. AS IV was purchased from Sigma-Aldrich (St. Louis, MO, USA), and dissolved in 100% ethanol, then diluted in 0.9% saline. Isoflurane was purchased from Abbott Laboratories (Lake Bluff, IL, USA). Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) apoptosis detection kit was from Roche Diagnostics (Rotkreuz, Switzerland). Antibodies against Akt (cat. no. 9272), phospho-Akt (p-Akt; cat. no. 4060), JNK (cat. no. 9252), p-JNK (cat. no. 4668), ERK (cat. no. 4695), p-ERK (cat. no. 4370), NF-κB p65 (cat. no. 8242), histone H3 (cat. no. 4499) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; cat. no. 5174) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Antibodies against caspase-3 (cat. no. ab2171), glycogen synthase kinase (GSK)-3β (cat. no. ab32391), p-GSK-3β (cat. no. ab75814) and Klotho (cat. no. ab181373) were purchased from Abcam, Inc. Anti-BCL2 was from Santa Cruz Biotechnology, Inc. (La Jolla, CA, USA). BCA protein quantification kit was from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). RIPA buffer and the IMS cell image analysis software was from Shanghai JRDUN Biotechnology, Co., Ltd., (Shanghai, China).

Experiment groups and anesthesia procedure

The rats (P5) were randomly divided into five groups (n=4/group), as follows: Inhaled isoflurane group; isoflurane + AS IV pre-treatment (10, 40 or 100 mg/kg) groups; and sham group without anesthesia. The rats were administered AS IV pre-treatment for three days at 0, 10, 40 or 100 mg/kg/day, then exposed to anesthesia. The sham group was pre-treated with ethanol in 0.9% saline vehicle. The general anesthetics procedure was performed on a platform over a water bath at 37–38°C. The isoflurane was flowed by oxygen at a concentration of 1.3%. The isoflurane concentration, oxygen concentration and CO2 concentration inside the incubator were regulated using a monitoring machine (Datex-Ohmeda, Inc., Madison, WI, USA). The rats were monitored for SpO2, skin color and breathing frequency. The body orientation was frequently changed to maintain SpO2 at >90% during the 3-h anesthesia. Sham group rats were placed in the incubator with air only.

Sample preparation, ELISA and biochemistry analysis

Rats were injected with phenobarbital (100 mg/kg) and sacrificed by CO2 asphyxiation at 12 h post-anesthesia, and placed on ice. Then the hippocampus tissues from rat brains were isolated. One part was fixed by 10% formalin for 12 h at 4°C, then transferred into 50% ethanol for 4–5 h. The tissues were processed for histology slides at 5-µm and subjected to hematoxylin and eosin (H&E) staining. Another part was dissolved in RIPA lysis buffer, and protein was isolated for western blot assay or to undergo biochemical analysis of enzymatic activities. Tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-1β levels were measured in blood and brain tissue collected at 12 h after isofluorane exposure using ELISA kits from R&D Systems, Inc. (Minneapolis, MN, USA). Blood samples were centrifuged at 3,000 × g for 10 min, and the serum was separated and stored at −80°C until assayed. For detecting TNF-α, IL-6 and IL-1β in brain tissue, frozen tissue samples were weighed and homogenized, then centrifuged at 12,000 × g for 10 min. The supernatants were stored at −80°C prior to analysis. The nitric oxide (NO) and malondialdehyde (MDA) levels and enzyme activities of nitric oxide synthase (iNOS) and superoxide dismutase (SOD) were quantified using analytical kits from the Nanjing Jiancheng Biological Engineering Institute (Nanjing, China), following manufacturers' instruction.

Western blot analysis

Brain tissues were cut into small pieces and homogenized in RIPA buffer containing protease and phosphatase inhibitors. The samples were centrifuged at 21,952 × g for 15 min at 4°C. The supernatant was quantified for protein concentration using the BCA method and stored at −80°C until use. The nuclear and cytosolic fractions were separated using the nuclear and cytoplasmic extraction buffers for detecting NF-κB p65 protein. Equal quantities of protein from each sample (80 µg) were loaded per lane and separated on 10% SDS-polyacrylamide gels. The resolved proteins were electrophoretically transferred to nitrocellulose membranes (EMD Millipore, Billerica, MA, USA), then blocked in 5% nonfat milk for 1 h, followed by overnight primary antibody incubation at 4°C. The primary antibodies used were anti-NF-κB p65 (1:1,000), anti-H3 (1:800), anti-Akt (1:1,000), anti-ERK (1:1,000), anti-JNK (1:1,000), anti-Bcl-2 (1:100), anti-GSK-3β (1:500), anti-Klotho (1:800) and anti-GAPDH (1:1,500). Subsequently the membranes were washed three times with Tris-buffered saline with 0.1% Tween-20, then incubated for 1 h with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (1:4,000; cat. no. ab6721; Abcam, Inc.) at room temperature. The membranes were then incubated with enhanced chemiluminescence detection reagents and exposed to X-ray films. The optical density of the bands was measured using the ChemiDoc™ Imaging System (Bio-Rad laboratories Inc., Hercules, CA, USA).

Apoptosis measurement

The TUNEL assay was performed using a commercially available kit (Thermo Fisher Scientific, Inc.), according to the manufacturer's protocol. Six CA1 areas from each slide were selected and analyzed for the positive cell number using the IMS cell image analysis system. TUNEL positive cells are apoptotic cells with dispersed distribution with condensed chromosomes, and manifested with brown particles in nucleus. The number of apoptotic cells in each field was counted for samples from different groups.

Statistic analysis

All the results were presented as the mean ± standard deviation. SPSS software, version 13.0 (SPSS, Inc., Chicago, IL, USA) was used for analysis. Differences among groups were compared using one-way analysis of variance, followed by Bonferroni post-hoc test. P<0.05 was considered to indicate a statistically significant difference.

Results

Isoflurane-induced neuron apoptosis in hippocampus CA1 region

We observed the tissue damage in hippocampus area in isofluorane-exposed rats, while there were few dead cells in hippocampus CA1 neuron in sham group rats (Fig. 1A and B); however, the number of dead cells showing shrunken cytoplasm and degenerated nuclei increased in tissues from the isoflurane-treated group. These results suggested that isoflurane exposure induced neuron cell death. By contrast, AS IV treatment (Fig. 1C-E) reduced isoflurane-induced neuron cell death. This indicated that AS IV exerts neuroprotective effects on isoflurane-induced neuron cell damage in the CA1 region of hippocampus in rats. To confirm whether the dead cells identified in the H&E stained tissues were apoptotic cells, we further examined cell apoptosis in these tissues by TUNEL assay (Fig. 2A). In the sham group, the neuron in hippocampus CA1 zone was rarely stained positive in the TUNEL assay. Isoflurane exposure significantly increased the apoptotic neuron numbers (P<0.05 vs. sham group, Fig. 2B). This indicates that the neurodegenerative damage caused by isoflurane was primarily a result of hippocampus neuron apoptosis. By contrast, AS IV treatment (40 and 100 mg/kg) significantly reduced isoflurane-induced neuron cell apoptosis (P<0.01).

Figure 1. Hematoxylin and eosin staining on hippocampus CA1 region tissues of newborn rats after isoflurane exposure with or without AS IV pre-treatment (magnification, ×10). (A) Sham, (B) isoflurane, (C) isoflurane + AS IV (10 mg/kg), (D) isoflurane + AS IV (40 mg/kg) and (E) isoflurane + AS IV (100 mg/kg). Note that a marked neuronal loss occurred in (B) compared with (A), whereas AS IV pre-treated groups (C-E) showed reduced neuron death compared with (B). AS IV, astragaloside IV.

Figure 2. Effect of AS IV on isoflurane-induced hippocampus neuron cell apoptosis. (A) Representative TUNEL staining of rat hippocampus tissues from different groups (magnification, ×40). (B) Numbers of TUNEL positive cells per area from hippocampus tissues of different groups. **P<0.01 vs. sham group; ##P<0.01 vs. AS IV (0 mg/kg). AS IV, astrogaloside IV.

SOD, iNOS, MDA and NO measurement in hippocampus tissue and rat serum

To test the levels of isoflurane-induced oxidative stress in different groups, we isolated rat serum and hippocampus tissues and measured the enzyme activities of a number of oxidative stress-associated antioxidant enzymes, including SOD, iNOS, the lipid peroxide marker MDA and NO. In the sham group, MDA levels were relatively low in the hippocampus CA1 zone and rat serum of the sham group (Figs. 3 and 4). Isoflurane exposure significantly inhibited the enzyme activities of total iNOS, SOD and NO (P<0.01 vs. sham), but increased MDA levels, which may contribute to the neuron apoptosis. By contrast, AS IV treatment (40 and 100 mg/kg) significantly inhibited isoflurane-induced MDA production and ameliorated the suppression of iNOS and SOD enzyme activity and NO production by isoflurane (Figs. 3 and 4).

Figure 3. AS IV inhibited isoflurane-induced nitrogen species production in hippocampus tissues. (A) SOD and (B) iNOS activity, (C) MDA and (D) NO levels detected in rat hippocampus tissues at 12 h post-anesthesia. **P<0.01 vs. Sham group; #P<0.05, ##P<0.01 vs. AS IV (0 mg/kg). AS IV, astragaloside IV; SOD, superoxide dismutase; iNOS, nitric oxide synthase; MDA, malondialdehyde; NO, nitric oxide.

Figure 4. AS IV inhibited isoflurane-induced nitrogen species production in rat serum. (A) SOD and (B) iNOS activity, (C) MDA and (D) NO levels detected in rat sera at 12 h post-anesthesia. **P<0.01 vs. Sham group; #P<0.05, ##P<0.01 vs. AS IV (0 mg/kg). AS IV, astragaloside IV; SOD, superoxide dismutase; iNOS, nitric oxide synthase; MDA, malondialdehdye; NO, nitric oxide.

Proinflammatory cytokine measurement in the serum and culture supernatant

To test the levels of isoflurane-induced proinflammatory responses in different groups, we isolated rat serum and measured levels of the major proinflammatory cytokines TNF-α, IL-6 and IL-1β (Fig. 5). In the sham group, these cytokine levels were relatively low in rat serum. Isoflurane exposure significantly increased the levels of all the three proinflammatory cytokines (P<0.01). By contrast, AS IV treatment (10, 40 and 100 mg/kg groups) significantly reduced isoflurane-induced proinflammatory cytokine release (P<0.01). These results suggested that isoflurane exposure induced systemic inflammatory responses in neonatal rats, and AS IV alleviated such inflammation.

Figure 5. AS IV reduced isoflurane-induced proinflammatory cytokine production in rat serum. (A) TNF-α, (B) IL-1β and (C) IL-6 levels detected in rat sera at 12 h post-anesthesia. **P<0.01 vs. Sham group; ##P<0.01 vs. AS IV (0 mg/kg). TNF-α, tumor necrosis factor-α; AS IV, astragaloside IV; IL, interleukin.

Inflammatory and antiapoptotic signaling pathway analysis

The proinflammatory cytokine responses and reactive nitrogen species production are primarily regulated by the NF-κB pathway. Thus, we measured the NF-κB activity in rat hippocampus tissues by western blot. In protein samples from the sham group, NF-κB protein was detected predominantly in the cytoplasm and levels were low in the nucleus, suggesting low NF-κB activity (Fig. 6A). Isoflurane exposure markedly increased nuclear levels of NF-κB protein, indicating NF-κB activation in hippocampus tissue. By contrast, AS IV treatment reduced isoflurane-induced NF-κB nuclear localization, indicating suppressed NF-κB activation (Fig. 6A).

Figure 6. Astragaloside IV (AS IV) attenuated isoflurane-induced NF-κB activation and apoptotic pathway activation in hippocampus tissues. Lane 1: Sham group; 2: Isoflurane group; 3: Isoflurane + AS IV (10 mg/kg); 4: Isoflurane + AS IV (40 mg/kg); and 5: Isoflurane + AS IV (100 mg/kg). (A) Cytoplasmic and nuclear NF-κB levels from rat hippocampus tissues. Histone H3 and GAPDH were used as loading control for nuclear protein and cytoplasmic protein, respectively. (B) Expression levels of apoptosis-related proteins in total cell lysates from rat hippocampus tissues were determined by western blot assay. GAPDH levels were used for equal loading control. NF-κB, nuclear factor-κB; Bcl-2, B-cell lymphoma 2; GSK-3β, glycogen synthase kinase 3β; JNK, c-Jun N-terminal kinase; ERK, extracellular signal-regulated kinase; AKT, protein kinase B; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

We further measured the expression levels of a number of apoptosis-associated proteins in different groups. In protein samples from the sham group, the expression of the proapoptotic marker protein caspase-3 was relatively low. By contrast, the antiapoptotic marker protein Bcl-2 was abundantly expressed. Isoflurane exposure markedly increased caspase-3 protein levels and decreased Bcl-2 protein levels (Fig. 6B). By contrast, AS IV treatment inhibited isoflurane-induced caspase-3 upregulation and Bcl-2 downregulation, suggesting that AS IV suppressed isoflurane-induced hippocampus apoptosis. Similarly, isoflurane exposure induced GSK-3β and decreased Klotho and phosphorylated Akt protein levels; however, AS IV pre-treatment inhibited isoflurane-induced changes in these proteins.

MAPK signaling pathways are intricately involved in apoptotic responses (23). We observed the activation of JNK and ERK by isoflurane exposure; indicated by increased phosphorylated protein levels. Furthermore, AS IV treatment inhibited isoflurane-induced MAPK activation (Fig. 6B).

Discussion

Apoptotic neuron cell death after anesthesia exposure has an important consequence in neurotoxicity (24). The two major pathways for cell apoptosis are the death receptor (Fas and TNFR)-mediated intrinsic pathway and mitochondria pathway (24). The mitochondria pathway can be activated by extrinsic and intrinsic stimuli, which includes oxidation and cytotoxic drug treatment. For the immature animal brain, particularly in specific development stages such as synaptogenesis period, general anesthesia can induce severe neurotoxicity, and mitochondria pathway activation is considered the initial step (5). The key step is cytochrome c release from mitochondria to the cytoplasm, and the mechanism is that isoflurane may activate IP3 receptor on endoplasmic reticulum and disrupt the calcium homeostasis, and changed the structure and function of the outer membrane-located Bcl-2 family proteins, mitochrondrial permeability transition, MPT and the channel (MPT pore) (25). Clinically, a mixture of laughing gas and isoflurane has been shown to activate the mitochondria pathway in neurons by downregulating antiapoptotic protein Bcl-xL, and immature brains are even more sensitive to isoflurane-induced neuron apoptosis by increasing proapoptotic protein Bax, decreasing antiapoptotic protein Bcl-2, and allowing free radical accumulation and cytochrome c release into cytoplasm, initiating apoptosis (26). A similar neurodegenerative effect was observed in the present study.

Oxidative stress, which is a result of the overproduction and accumulation of free radicals, including ROS and RNS, plays a major role in a variety of neurological pathogenesis (27). Disruption of the balance of free radical generation and antioxidant enzyme system may result in oxidative stress. Previous studies have reported that AS IV is an effective free radical scavenger and potentiates the intrinsic antioxidant system in the animal model of cerebral ischemia/reperfusion (16). In the present study, the results indicated that AS IV enhanced intrinsic antioxidant enzyme activity and decreased RNS accumulation in the hippocampus tissue and serum.

Notably, we also observed a systemic increase of proinflammatory cytokines (TNF-α, IL-1β and IL-6) in neonatal rats following isoflurane exposure, suggesting an inflammatory responses triggered by the anesthesia procedure. AS IV also exhibited anti-inflammatory activity, in this case by alleviating anesthesia-induced proinflammatory cytokine production. As a master regulator of inflammatory responses and cell survival, NF-κB activation has been documented in multiple neurological pathology conditions (28). Although NF-κB is primarily a survival factor in numerous cell types, its role in neuronal apoptosis may vary depending on the specific contexts and treatments (29). In the present study, we observed isoflurane-induced NF-κB activation, which could be from accumulated oxidative stress. Crucially, AS IV reduced such NF-κB activation, and ameliorated systemic proinflammatory cytokine production.

It has been reported that isoflurane exposure downregulates Bcl-2 and ERK signaling in the developing rat brain (30). However, we identified increased level of phosphorylated ERK and JNK in neuron cells after isoflurane exposure. The JNK pathway is generally considered a proapoptotic pathway, while ERK signaling is often considered neuroprotective (23). However, MAPKs are often activated simultaneously in response to stress or stimulation (23). Thus, we consider ERK activation as an early reaction in response to isoflurane exposure; however, its neuroprotective effect may be masked by other signaling pathways such as JNK pathway.

AS IV is a potent agent for antioxidant and ROS clearance. AS IV may inhibit oxidized product formation in cell membrane and nuclear DNA adducts, reduce the accumulation of ROS inside the mitochondria and alleviate oxidative stress-induced cell apoptosis (17,21). AS IV has been speculated to exert a protective effect in treating degenerative diseases in central neural system, such as Alzheimer's disease, Parkinson's disease, ischemia-reperfusion-induced brain damage, and physical damage in the brain (15,22,31,32). The results of the present study confirmed that AS IV is protective against isoflurane-induced neuronal degeneration in the developing rat brain. The underlying mechanism may be associated with the inhibition of oxidative stress and inflammation in the hippocampus region and systemic level, thereby reducing abnormal apoptosis of developing neuron. Thus, the antiapoptotic and antioxidative function of AS IV in general anesthesia-induced neuron toxicity may have potential clinical relevance.

References