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Article

Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis

  • Authors:
    • Lufang Chen
    • Keying Xu
    • Qifeng Gui
    • Yue Chen
    • Deying Chen
    • Yunmei Yang
  • View Affiliations / Copyright

    Affiliations: Department of Geriatrics, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, State Key Laboratory and Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
  • Pages: 1836-1842
    |
    Published online on: July 20, 2016
       https://doi.org/10.3892/etm.2016.3534
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Abstract

A number of clinical trials have demonstrated that the use of probiotics has the potential to prevent nosocomial infections. However, the mechanism underlying probiotic-induced anti‑infection and sepsis remains to be investigated. In the present study, 200 µl/day of Lactobacillus rhamnosus GG (LGG) or normal saline (control) was orally administrated to 4-week-old C57BL6 mice 4 weeks prior to cecal ligation and puncture (CLP). A number of mice were sacrificed 24 h after CLP, and the remaining mice were used for survival studies. Ileum tissues were collected to evaluate the injury on the intestine. Blood samples were also obtained to investigate the changed metabolic pattern in mice that underwent different treatments using ultra‑performance liquid chromatography coupled with quadrupole time‑of‑flight mass spectrometry (UPLC‑QTOF‑MS). In the survival studies, the mortality of CLP‑induced septic mice pretreated with LGG was significantly lower compared with untreated mice (P=0.029). Ileum mucosal damage was evident in the control septic mice. Based on the data of UPLC‑QTOF‑MS, phosphatidylcholines were increased and lysophosphatidylcholines (LPCs) that contained polyunsaturated fatty acids were decreased in septic mice, whereas saturated fatty acid LPCs reveal no significant difference between septic and sham mice. In addition, the metabolic profile in the septic mice pretreated with LGG was much closer to that of sham mice compared with control septic mice. The results of the present study suggest that probiotic pre‑administration reduces the mortality in septic mice by decreasing ileum mucosal damage, increasing the gut barrier integrity and altering global serum metabolic profiles.
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Copy and paste a formatted citation
Spandidos Publications style
Chen L, Xu K, Gui Q, Chen Y, Chen D and Yang Y: Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis. Exp Ther Med 12: 1836-1842, 2016.
APA
Chen, L., Xu, K., Gui, Q., Chen, Y., Chen, D., & Yang, Y. (2016). Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis. Experimental and Therapeutic Medicine, 12, 1836-1842. https://doi.org/10.3892/etm.2016.3534
MLA
Chen, L., Xu, K., Gui, Q., Chen, Y., Chen, D., Yang, Y."Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis". Experimental and Therapeutic Medicine 12.3 (2016): 1836-1842.
Chicago
Chen, L., Xu, K., Gui, Q., Chen, Y., Chen, D., Yang, Y."Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1836-1842. https://doi.org/10.3892/etm.2016.3534
Copy and paste a formatted citation
x
Spandidos Publications style
Chen L, Xu K, Gui Q, Chen Y, Chen D and Yang Y: Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis. Exp Ther Med 12: 1836-1842, 2016.
APA
Chen, L., Xu, K., Gui, Q., Chen, Y., Chen, D., & Yang, Y. (2016). Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis. Experimental and Therapeutic Medicine, 12, 1836-1842. https://doi.org/10.3892/etm.2016.3534
MLA
Chen, L., Xu, K., Gui, Q., Chen, Y., Chen, D., Yang, Y."Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis". Experimental and Therapeutic Medicine 12.3 (2016): 1836-1842.
Chicago
Chen, L., Xu, K., Gui, Q., Chen, Y., Chen, D., Yang, Y."Probiotic pre-administration reduces mortality in a mouse model of cecal ligation and puncture-induced sepsis". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1836-1842. https://doi.org/10.3892/etm.2016.3534
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