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Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk

  • Authors:
    • Sertaç Argun Kıvanç
    • Merih Kıvanç
    • Tülay Yiğit
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, School of Medicine, Uludağ University, Görükle, 16059 Bursa, Turkey, Department of Microbiology, Faculty of Science, Anadolu University, Eskişehir 26470, Turkey, Institute of Science, Anadolu University, Eskişehir 26470, Turkey
    Copyright: © Kıvanç et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1732-1740
    |
    Published online on: July 26, 2016
       https://doi.org/10.3892/etm.2016.3545
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Abstract

Enterococci, which have useful biotechnological applications, produce bacteriocins, including those that exert anti-Listerial activity. The present study aimed to determine the antibiotic susceptibility patterns and antimicrobial activity of Enterococcus faecium strains isolated from human breast milk. The strains were identified using carbohydrate fermentation tests and ribotyping. Subsequently, the antibacterial activity of the isolates was investigated, and the quantities of lactic acid and hydrogen peroxide produced, and the proteolytic activity of E. faecium, were determined. In addition, biofilm formation by E. faecium strains was assessed. E. faecium strains exhibited antimicrobial activity against food‑borne and clinical bacterial isolates. Furthermore, following 24 h incubation, the tested strains exhibited resistance to a pH range of 2.0‑9.5 and tolerance of bile acid, lysozyme activity and phenol. Supernatants of the E. faecium TM13, TM15, TM17 and TM18 strains were shown to be effective against Listeria monocytogenes, and were also resistant to heat. Further studies are required in order to determine whether certain strains of E. faecium may be used for the development of novel antibacterial agents.
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1 

Lindemann PC, Foshaugen I and Lindemann R: Characteristics of breast milk and serology of women donating breast milk to a milk bank. Arc Dis Child Fetal Neonatal Ed. 8:F440–F441. 2004. View Article : Google Scholar

2 

Martin R, Langa S, Reviriego C, Jiménez E, Marín ML, Olivares M, Boza J, Jiménez J, Fernández L, Xuas J and Rodríguez JM: The commensal microflora of human milk: New perspectives for food bacteriotherapy and probiotics. Trends Food Sci Technol. 15:121–127. 2004. View Article : Google Scholar

3 

Saavedra JM: Clinical applications of probiotic agents. Am J Clin Nutr. 73:1147S–1151S. 2001.PubMed/NCBI

4 

Ip S, Chung M, Raman G, Chew P, Magula N, DeVine D, Trikalinos T and Lau J: Breastfeeding and maternal and infant health outcomes in developed countries. Evid Rep Technol Assess (Full Rep). 1–186. 2007.

5 

Labbok M: Breastfeeding: A woman's reproductive right. Int J Gynaecol Obstet. 94:277–286. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Giraffa G: Functionality of enterococci in dairy products. Int J Food Microbiol. 88:215–222. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Foulquié Moreno MR, Sarantinopoulos P, Tsakalidou EL and De Vuyst L: The role and application of enterococci in food and health. Int J Food Microbiol. 106:1–24. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Cocolin L, Foschino R, Comi G and Fortina M: Description of the bacteriocins produced by two strains of Enterococcus faecium isolated from Italian goat milk. Food Microbiol. 24:752–758. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Schillinger U and Lucke FK: Identification of lactobacilli from meat and meat products. Food Microbiol. 4:199–208. 1987. View Article : Google Scholar

10 

Stiles ME and Holzapfel WH: Lactic acid bacteria of foods and their current taxonomy. Int J Food Microbiol. 36:1–29. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Creti R, Imperi M, Bertuccini L, Fabretti F, Orefici G, Di Rosa R and Baldassarri L: Survey for virulence determinants among Enterococus faecalis isolated from different sources. J Med Microbiol. 53:13–20. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Stepanovic S, Vukovic D, Dakic I, Savić B and Svabic-Vlahovic M: A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods. 40:175–179. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Christensen GD, Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM and Beachey EH: Adherence of coagulase-negative staphylococci to plastic tissue culture plates: A quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 22:996–1006. 1985.PubMed/NCBI

14 

Demirci M and Gunduz H: Dairy Technology Handbook. Hasad Press; Turkey: pp. 1841994

15 

Citti J, Sandine WE and Elliker PR: Some observations on the Hull method for measurement of proteolysis in milk. J Dairy Sci. 46:3371963. View Article : Google Scholar

16 

Rajagopal SN and Sandine WE: Fissociative growth and proteolysis of Streptococcus thermophilus and Lactobacillus bulgaricus in Skim Milk. J Dairy Sci. 73:894–899. 1990. View Article : Google Scholar

17 

Patrick WA and Wagner HB: Determination of hydrogen peroxide in small concentrations. Anal Chem. 21:1279–1280. 1949. View Article : Google Scholar

18 

Clinical and Laboratory Standards Institute, . Performance Standards for Antimimicrobial Susceptibility TestingTwenty-Third Informational Supplement. CLSI; Wayne, PA: pp. 1992013

19 

Morandi S, Brasca M, Andrighetto C, Lombardi A and Lodi R: Technological and molecular characterization of enterococci isolated from north-west Italian dairy products. Int Dairy J. 16:867–875. 2006. View Article : Google Scholar

20 

Martín R, Langa S, Reviriego C, Jimínez E, Marín ML, Xaus J, Fernández L and Rodríguez JM: Human milk is a source of lactic acid bacteria for the infant gut. J Pediatr. 143:754–758. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Shoughy SS and Tabbara KF: Listeria monocytogenes endophthalmitis following keratoconjunctivitis. Clin Ophthalmol. 8:301–304. 2014.PubMed/NCBI

22 

Gandhi M and Chikindas ML: Listeria: A foodborne pathogen that knows how to survive. Int J Food Microbiol. 113:1–15. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Kang JH and Lee MS: Characterization of a bacteriocin produced by Enterococcus faecium GM-1 isolated from an infant. J Appl Microbiol. 98:1169–1176. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Ohmomo S, Murata S, Katayama N, Nitisinprasart S, Kobayashi M, Nakajima T, Yajima M and Nakanishi K: Purification and some characteristics of enterocin ON-157, a bacteriocin produced by Enterococcus faecium NIAI 157. J Appl Microbiol. 88:81–89. 2000. View Article : Google Scholar : PubMed/NCBI

25 

Mannu L, Paba A, Daga E, Comunian R, Zanetti S, Duprè I and Sechi LA: Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int J Food Microbiol. 88:291–304. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Banwo K, Sanni A and Tan H: Technological properties and probiotic potential of Enterococcus faecium strains isolated from cow milk. J Appl Microbiol. 114:229–241. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Andrighetto C, Knijff E, Lombardi A, Torriani S, Vancanneyt M, Kersters K, Swings J and Dellaglio F: Phenotypic and genetic diversity of enterococci isolated from Italian cheeses. J Dairy Res. 68:303–316. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Vassos D, Bezırtzoglou EA, Voıdarou C, Alexopoulos A and Maıpa V: Biochemical and antimicrobial profile of Enterococcus faecium and E. faecalis isolated from traditional dairy products and infant intestinal microbiota. Microb Ecol Health Dis. 21:241–250. 2009. View Article : Google Scholar

29 

Bottazzi V, Battistotti B, Bosi F, Corradini C and Dell'Acqua E: Effects of lysozyme on the thermophilous lactic ferments. 20th International Dairy Congress. Paris. pp. 535–536. 1978; (In French).

30 

Di Rosa R, Creti R, Venditti M, D'Amelio R, Arciola CR, Montanaro L and Baldassarri L: Relationship between biofilm formation, the enterococcal surface protein (Esp) and gelatinase in clinical isolates of Enterococcus faecalis and Enterococcus faecium. FEMS Microbiol Lett. 256:145–150. 2006. View Article : Google Scholar

31 

Arizcun C, Barcina Y and Torre P: Identification and characterization of proteolytic activity of Enterococcus spp. isolated from milk and Roncal and Idiazábal cheese. Int J Food Microbiol. 38:17–24. 1997. View Article : Google Scholar : PubMed/NCBI

32 

Suzzi G, Caruso M, Gardini F, Lombardi A, Vannini L, Guerzoni ME, Andrighetto C and Lanorte MT: A survey of the enterococci isolated from an artisanal Italian goat's cheese (semicotto caprino). J Appl Microbiol. 89:267–274. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Sarantinopoulos P, Kalantzopoulos G and Tsakalidou E: Effect of Enterococcus faecium on microbiological, physicochemical and sensory characteristics of Greek Feta cheese. Int J Food Microbiol. 76:93–105. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Juven BJ, Weisslowicz H and Harel S: Detection of hydrogen peroxide produced by meat lactic starter cultures. J Appl Bacteriol. 65:357–360. 1988. View Article : Google Scholar

35 

Cotter PD, Hill C and Ross RP: Bacteriocins: Developing innate immunity for food. Nat Rev Microbiol. 3:777–788. 2005. View Article : Google Scholar : PubMed/NCBI

36 

Perez R H, Zendo T and Sonomoto K: Novel bacteriocins from lactic acid bacteria (LAB): Various structures and applications. Microb Cell Fact. 13(Suppl 1): S32014. View Article : Google Scholar

37 

Klaenhammer TR: Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev. 12:39–85. 1993. View Article : Google Scholar : PubMed/NCBI

38 

Yousif NM, Dawyndt P, Abriouel H, Wijaya A, Schillinger U, Vancanneyt M, Swings J, Dirar HA, Holzapfel WH and Franz CM: Molecular characterization, technological properties and safety aspects of enterococci from ‘Hussuwa’, an African fermented sorghum product. J Appl Microbiol. 98:216–228. 2005. View Article : Google Scholar : PubMed/NCBI

39 

Billström H, Top J, Edlund C and Lund B: Frequent occurrence of multidrug-resistant CC17 Enterococcus faecium among clinical isolates in Sweden. J Appl Microbiol. 108:1810–1816. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kıvanç SA, Kıvanç M and Yiğit T: Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk. Exp Ther Med 12: 1732-1740, 2016.
APA
Kıvanç, S.A., Kıvanç, M., & Yiğit, T. (2016). Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk. Experimental and Therapeutic Medicine, 12, 1732-1740. https://doi.org/10.3892/etm.2016.3545
MLA
Kıvanç, S. A., Kıvanç, M., Yiğit, T."Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk". Experimental and Therapeutic Medicine 12.3 (2016): 1732-1740.
Chicago
Kıvanç, S. A., Kıvanç, M., Yiğit, T."Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1732-1740. https://doi.org/10.3892/etm.2016.3545
Copy and paste a formatted citation
x
Spandidos Publications style
Kıvanç SA, Kıvanç M and Yiğit T: Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk. Exp Ther Med 12: 1732-1740, 2016.
APA
Kıvanç, S.A., Kıvanç, M., & Yiğit, T. (2016). Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk. Experimental and Therapeutic Medicine, 12, 1732-1740. https://doi.org/10.3892/etm.2016.3545
MLA
Kıvanç, S. A., Kıvanç, M., Yiğit, T."Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk". Experimental and Therapeutic Medicine 12.3 (2016): 1732-1740.
Chicago
Kıvanç, S. A., Kıvanç, M., Yiğit, T."Antibiotic susceptibility, antibacterial activity and characterisation of Enterococcus faecium strains isolated from breast milk". Experimental and Therapeutic Medicine 12, no. 3 (2016): 1732-1740. https://doi.org/10.3892/etm.2016.3545
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