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ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway

  • Authors:
    • Zhe-Xun Lian
    • Fang Wang
    • Jun-Hua Fu
    • Zuo-Yuan Chen
    • Hui Xin
    • Ru-Yong Yao
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Cardiology, The Third People's Hospital of Qingdao, Qingdao, Shandong 266000, P.R. China, Department of Interventional Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China, Department of Central Laboratory, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China
    Copyright: © Lian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2063-2068
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    Published online on: August 2, 2016
       https://doi.org/10.3892/etm.2016.3563
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Abstract

The aim of the present study was to examine the post-infarct acute effect of adenosine-5'-triphosphate (ATP) on myocardial infarction (MI) size as well as its precise molecular mechanism. Sixty New Zealand white male rabbits were exposed to 40 min of ischemia followed by 180 min of reperfusion. The rabbits were intravenously administered 3 mg/kg of ATP (ATP group) or saline (control group) immediately after reperfusion and maintained throughout the first 30 min. The wortmannin+ATP, PD-98059+ATP, and 5-hydroxydecanoic acid (5-HD) sodium salt+ATP groups were separately injected with wortmannin (0.6 mg/kg), PD-98059 (0.3 mg/kg), and 5-HD (5 mg/kg) 5 min prior to ATP administration. MI size was calculated as the percentage of the risk area in the left ventricle. Myocardial apoptosis was determined using a TUNEL assay. Western blot analysis was performed to examine the levels of protein kinase B (Akt)/p-Akt and extracellular signal-regulated kinase (ERK)/p-ERK in the ischemic myocardium, 180 min after reperfusion. The infarct size was significantly smaller in the ATP group than in the control group (p<0.05). The infarct size-reducing effect of ATP was completely blocked by wortmannin, PD-98059 and 5-HD. Compared with the control group, cardiomyocyte apoptosis was significantly reduced in the ATP group, while this did not occur in the wortmannin+ATP, PD-98059+ATP and 5-HD+ATP groups. Western blot analysis revealed a higher myocardial expression of p-Akt and p-ERK 180 min following reperfusion in the ATP versus the control group. In conclusion, cardioprotection by postischemic ATP administration is mediated through activation of the reperfusion injury salvage kinase (RISK) pathway and opening of the mitochondrial ATP-dependent potassium channels.
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1 

Yellon DM and Baxter GF: Protecting the ischaemic and reperfused myocardium in acute myocardial infarction: distant dream or near reality? Heart. 83:381–387. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Hearse DJ and Bolli R: Reperfusion induced injury: manifestations, mechanisms, and clinical relevance. Cardiovasc Res. 26:101–108. 1992. View Article : Google Scholar : PubMed/NCBI

3 

Yellon DM and Baxter GF: Reperfusion injury revisited: is there a role for growth factor signaling in limiting lethal reperfusion injury? Trends Cardiovasc Med. 9:245–249. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Kuno A, Solenkova NV, Solodushko V, Dost T, Liu Y, Yang XM, Cohen MV and Downey JM: Infarct limitation by a protein kinase G activator at reperfusion in rabbit hearts is dependent on sensitizing the heart to A2b agonists by protein kinase C. Am J Physiol Heart Circ Physiol. 295:H1288–H1295. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Urmaliya VB, Pouton CW, Ledent C, Short JL and White PJ: Cooperative cardioprotection through adenosine A1 and A2A receptor agonism in ischemia-reperfused isolated mouse heart. J Cardiovasc Pharmacol. 56:379–388. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Kin H, Zatta AJ, Lofye MT, Amerson BS, Halkos ME, Kerendi F, Zhao ZQ, Guyton RA, Headrick JP and Vinten-Johansen J: Postconditioning reduces infarct size via adenosine receptor activation by endogenous adenosine. Cardiovasc Res. 67:124–133. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Regan SE, Broad M, Byford AM, Lankford AR, Cerniway RJ, Mayo MW and Matherne GP: A1 adenosine receptor overexpression attenuates ischemia-reperfusion-induced apoptosis and caspase 3 activity. Am J Physiol Heart Circ Physiol. 284:H859–H866. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Philipp S, Yang XM, Cui L, Davis AM, Downey JM and Cohen MV: Postconditioning protects rabbit hearts through a protein kinase C-adenosine A2b receptor cascade. Cardiovasc Res. 70:308–314. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Erga KS, Seubert CN, Liang HX, Wu L, Shryock JC and Belardinelli L: Role of A(2A)-adenosine receptor activation for ATP-mediated coronary vasodilation in guinea-pig isolated heart. Br J Pharmacol. 130:1065–1075. 2000. View Article : Google Scholar : PubMed/NCBI

10 

Lian ZX, Liu F, Liu S, Xin H, Chen ZY, Tian JH, An Y and Cai SL: Cardioprotection of ischemic postconditioning and ATP-postconditioning in rabbits is associated with the activation of adenosine receptors. Eur Heart J. 27:(Suppl 1). 722006.

11 

Hausenloy DJ, Tsang A and Yellon DM: The reperfusion injury salvage kinase pathway: a common target for both ischemic preconditioning and postconditioning. Trends Cardiovasc Med. 15:69–75. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Bopassa J-C, Ferrera R, Gateau-Roesch O, Couture-Lepetit E and Ovize M: PI 3-kinase regulates the mitochondrial transition pore in controlled reperfusion and postconditioning. Cardiovasc Res. 69:178–185. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Kin H, Wang NP, Mykytenko J, Reeves J, Deneve J, Jiang R, Zatta AJ, Guyton RA, Vinten-Johansen J and Zhao ZQ: Inhibition of myocardial apoptosis by postconditioning is associated with attenuation of oxidative stress-mediated nuclear factor-kappa B translocation and TNF alpha release. Shock. 29:761–768. 2008.PubMed/NCBI

14 

Fleetwood G, Coade SB, Gordon JL and Pearson JD: Kinetics of adenine nucleotide catabolism in coronary circulation of rats. Am J Physiol. 256:H1565–H1572. 1989.PubMed/NCBI

15 

Nanto S, Kitakaze M, Takano Y, Hori M and Nagata S: Intracoronary administration of adenosine triphosphate increases myocardial adenosine levels and coronary blood flow in man. Jpn Circ J. 61:836–842. 1997. View Article : Google Scholar : PubMed/NCBI

16 

Olivetti G, Quaini F, Sala R, Lagrasta C, Corradi D, Bonacina E, Gambert SR, Cigola E and Anversa P: Acute myocardial infarction in humans is associated with activation of programmed myocyte cell death in the surviving portion of the heart. J Mol Cell Cardiol. 28:2005–2016. 1996. View Article : Google Scholar : PubMed/NCBI

17 

Vilahur G, Cubedo J, Casani L, Padro T, Sabate-Tenas M, Badimon JJ and Badimon L: Reperfusion-triggered stress protein response in the myocardium is blocked by post-conditioning. Systems biology pathway analysis highlights the key role of the canonical aryl-hydrocarbon receptor pathway. Eur Heart J. 34:2082–2093. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Heusch G, Boengler K and Schulz R: Cardioprotection: nitric oxide, protein kinases, and mitochondria. Circulation. 118:1915–1919. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Goodman MD, Koch SE, Fuller-Bicer GA and Butler KL: Regulating RISK: a role for JAK-STAT signaling in postconditioning? Am J Physiol Heart Circ Physiol. 295:H1649–H1656. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Zhu M, Feng J, Lucchinetti E, Fischer G, Xu L, Pedrazzini T, Schaub MC and Zaugg M: Ischemic postconditioning protects remodeled myocardium via the PI3K-PKB/Akt reperfusion injury salvage kinase pathway. Cardiovasc Res. 72:152–162. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Yang XM, Krieg T, Cui L, Downey JM and Cohen MV: NECA and bradykinin at reperfusion reduce infarction in rabbit hearts by signaling through PI3K, ERK, and NO. J Mol Cell Cardiol. 36:411–421. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Cohen MV, Yang XM, Liu Y, Solenkova NV and Downey JM: Cardioprotective PKG-independent NO signaling at reperfusion. Am J Physiol Heart Circ Physiol. 299:H2028–H2036. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Hide EJ and Thiemermann C: Limitation of myocardial infarct size in the rabbit by ischaemic preconditioning is abolished by sodium 5-hydroxydecanoate. Cardiovasc Res. 31:941–946. 1996. View Article : Google Scholar : PubMed/NCBI

24 

Yang XM, Proctor JB, Cui L, Krieg T, Downey JM and Cohen MV: Multiple, brief coronary occlusions during early reperfusion protect rabbit hearts by targeting cell signaling pathways. J Am Coll Cardiol. 44:1103–1110. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Han J, Kim N, Joo H, Kim E and Earm YE: ATP-sensitive K(+) channel activation by nitric oxide and protein kinase G in rabbit ventricular myocytes. Am J Physiol Heart Circ Physiol. 283:H1545–H1554. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Burnstock G: Overview: purinergic receptorsRole of adenosine and adenosine nucleotides in the biological system. Imai S and Nakazawa M: Elsevier Science; Amsterdam: pp. 1–6. 1991

27 

Komamura K, Ito H, Takiuchi S, Iwakura K, Maruyama A, Masuyama T, Minamino T, Node K, Kitakaze M and Hori M: Transient intracoronary infusion of ATP after reperfusion reduces the extent of no-reflow and infarct size in dogs. J Am Coll Cardiol. 25:227A–228A. 1995. View Article : Google Scholar

28 

Mahaffey KW, Puma JA, Barbagelata NA, DiCarli MF, Leesar MA, Browne KF, Eisenberg PR, Bolli R, Casas AC, Molina-Viamonte V, et al: Adenosine as an adjunct to thrombolytic therapy for acute myocardial infarction: results of a multicenter, randomized, placebo-controlled trial: the Acute Myocardial Infarction STudy of ADenosine (AMISTAD) trial. J Am Coll Cardiol. 34:1711–1720. 1999. View Article : Google Scholar : PubMed/NCBI

29 

Ross AM, Gibbons RJ, Stone GW, Kloner RA and Alexander RW: AMISTAD-II Investigators: A randomized, double-blinded, placebo-controlled multicenter trial of adenosine as an adjunct to reperfusion in the treatment of acute myocardial infarction (AMISTAD-II). J Am Coll Cardiol. 45:1775–1780. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Kloner RA, Forman MB, Gibbons RJ, Ross AM, Alexander RW and Stone GW: Impact of time to therapy and reperfusion modality on the efficacy of adenosine in acute myocardial infarction: the AMISTAD-2 trial. Eur Heart J. 27:2400–2405. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Miyagawa M, Kumano S, Sekiya M, Watanabe K, Akutzu H, Imachi T, Tanada S and Hamamoto K: Thallium-201 myocardial tomography with intravenous infusion of adenosine triphosphate in diagnosis of coronary artery disease. J Am Coll Cardiol. 26:1196–1201. 1995. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lian Z, Wang F, Fu J, Chen Z, Xin H and Yao R: ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway. Exp Ther Med 12: 2063-2068, 2016.
APA
Lian, Z., Wang, F., Fu, J., Chen, Z., Xin, H., & Yao, R. (2016). ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway. Experimental and Therapeutic Medicine, 12, 2063-2068. https://doi.org/10.3892/etm.2016.3563
MLA
Lian, Z., Wang, F., Fu, J., Chen, Z., Xin, H., Yao, R."ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway". Experimental and Therapeutic Medicine 12.4 (2016): 2063-2068.
Chicago
Lian, Z., Wang, F., Fu, J., Chen, Z., Xin, H., Yao, R."ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2063-2068. https://doi.org/10.3892/etm.2016.3563
Copy and paste a formatted citation
x
Spandidos Publications style
Lian Z, Wang F, Fu J, Chen Z, Xin H and Yao R: ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway. Exp Ther Med 12: 2063-2068, 2016.
APA
Lian, Z., Wang, F., Fu, J., Chen, Z., Xin, H., & Yao, R. (2016). ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway. Experimental and Therapeutic Medicine, 12, 2063-2068. https://doi.org/10.3892/etm.2016.3563
MLA
Lian, Z., Wang, F., Fu, J., Chen, Z., Xin, H., Yao, R."ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway". Experimental and Therapeutic Medicine 12.4 (2016): 2063-2068.
Chicago
Lian, Z., Wang, F., Fu, J., Chen, Z., Xin, H., Yao, R."ATP-induced cardioprotection against myocardial ischemia/reperfusion injury is mediated through the RISK pathway". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2063-2068. https://doi.org/10.3892/etm.2016.3563
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