Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Experimental and Therapeutic Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-0981 Online ISSN: 1792-1015
Journal Cover
October-2016 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2016 Volume 12 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity

  • Authors:
    • Fengyong Yang
    • Xiujing Qi
    • Zheng Gao
    • Xingju Yang
    • Xingfeng Zheng
    • Chonghao Duan
    • Jian Zheng
  • View Affiliations / Copyright

    Affiliations: Intensive Care Unit, The People's Hospital of Laiwu, Laiwu, Shandong 271199, P.R. China, Burn Center, Shanghai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Department of Thoracic Surgery, The People's Hospital of Laicheng, Laiwu, Shandong 271199, P.R. China
  • Pages: 2247-2252
    |
    Published online on: August 2, 2016
       https://doi.org/10.3892/etm.2016.3564
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the role of homocysteine (Hcy) in the pathogenesis of pulmonary embolism (PE) and the associated molecular mechanisms in human umbilical vein endothelial cells (HUVECs). Hcy contents were detected with high‑performance liquid chromatography. Apoptosis was detected by flow cytometry using Annexin‑V staining. Cytochrome c oxidase (COX) activity was assessed with an enzyme activity assay, and the expression levels of COX 17 were determined by western blot analysis. Intracellular reactive oxygen species levels were measured using a microplate reader with a fluorescence probe. The results demonstrated that, compared with the control group, the serum Hcy levels were significantly elevated in the PE group, suggesting that Hcy may be an indicator for PE. Following treatment with Hcy, the apoptosis rate was markedly elevated in HUVECs. Moreover, Hcy decreased COX activity and downregulated the expression of COX 17 in HUVECs. Furthermore, Hcy increased the ROS levels in these endothelial cells. However, all the above‑mentioned physiopathological changes induced by Hcy in HUVECs could be restored by folic acid. In conclusion, the results of the present study demonstrated that Hcy inhibited COX activity, downregulated COX 17 expression, increased intracellular ROS levels and enhanced apoptosis in endothelial cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

de Miguel-Díez J, Jiménez-García R, de Andrés A López, Hernández-Barrera V, Carrasco-Garrido P, Monreal M, Jiménez D, Jara-Palomares L and Álvaro-Meca A: Analysis of environmental risk factors for pulmonary embolism: A case-crossover study (2001-2013). Eur J Intern Med. 31:55–61. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Heit JA: The epidemiology of venous thromboembolism in the community: Implications for prevention and management. J Thromb Thrombolysis. 21:23–29. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Tapson VF: Acute pulmonary embolism. N Engl J Med. 358:1037–1052. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Chen P, Poddar R, Tipa EV, Dibello PM, Moravec CD, Robinson K, Green R, Kruger WD, Garrow TA and Jacobsen DW: Homocysteine metabolism in cardiovascular cells and tissues: Implications for hyperhomocysteinemia and cardiovascular disease. Adv Enzyme Regul. 39:93–109. 1999. View Article : Google Scholar : PubMed/NCBI

5 

Jeremy JY, Shukla N, Angelini GD, Day A, Wan IY, Talpahewa SP and Ascione R: Sustained increases of plasma homocysteine, copper and serum ceruloplasmin after coronary artery bypass grafting. Ann Thorac Surg. 74:1553–1557. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Karalezli A, Parlak ES, Kanbay A, Senturk A and Hasanoglu HC: Homocysteine and serum-lipid levels in pulmonary embolism. Clin Appl Thromb Hemost. 17:E186–E189. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Köktürk N, Kanbay A, Aydoğdu M, Özyılmaz E, Bukan N and Ekim N: Hyperhomocysteinemia prevalence among patients with venous thromboembolism. Clin Appl Thromb Hemost. 17:487–493. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Okumus G, Kiyan E, Arseven O, Tabak L, DizKucukkaya R, Unlucerci Y, Abaci N, Unaltuna NE and Issever H: Hereditary thrombophilic risk factors and venous thromboembolism in Istanbul, Turkey: The role in different clinical manifestations of venous thromboembolism. Clin Appl Thromb Hemost. 14:168–173. 2008. View Article : Google Scholar : PubMed/NCBI

9 

SallesCrawley II, Monkman JH, Ahnström J, Lane DA and Crawley JT: Vessel wall BAMBI contributes to hemostasis and thrombus stability. Blood. 123:2873–2881. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Ren M, Li R, Luo M, Chen N, Deng X, Yan K, Zeng M and Wu J: Endothelial cells but not platelets are the major source of Toll-like receptor 4 in the arterial thrombosis and tissue factor expression in mice. Am J Physiol Regul Integr Comp Physiol. 307:R901–R907. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Mansoor MA, Bergmark C, Haswell SJ, Savage IF, Evans PH, Berge RK, Svardal AM and Kristensen O: Correlation between plasma total homocysteine and copper in patients with peripheral vascular disease. Clin Chem. 46:385–391. 2000.PubMed/NCBI

12 

Shukla N, Angelini GD and Jeremy JY: Interactive effects of homocysteine and copper on angiogenesis in porcine isolated saphenous vein. Ann Thorac Surg. 84:43–49. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Garnier A, Fortin D, Deloménie C, Momken I, Veksler V and Ventura-Clapier R: Depressed mitochondrial transcription factors and oxidative capacity in rat failing cardiac and skeletal muscles. J Physiol. 551:491–501. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Cawthon D, McNew R, Beers KW and Bottje WG: Evidence of mitochondrial dysfunction in broilers with pulmonary hypertension syndrome (Ascites): Effect of t-butyl hydroperoxide on hepatic mitochondrial function, glutathione and related thiols. Poult Sci. 78:114–124. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Cawthon D, Beers K and Bottje WG: Electron transport chain defect and inefficient respiration may underlie pulmonary hypertension syndrome (ascites)-associated mitochondrial dysfunction in broilers. Poult Sci. 80:474–484. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Suter M, Remé C, Grimm C, Wenzel A, Jäättela M, Esser P, Kociok N, Leist M and Richter C: Age-related macular degeneration. The lipofusion component N-retinyl-N-retinylidene ethanolamine detaches proapoptotic proteins from mitochondria and induces apoptosis in mammalian retinal pigment epithelial cells. J Biol Chem. 275:39625–39630. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Srinivasan S and Avadhani NG: Cytochrome c oxidase dysfunction in oxidative stress. Free Radic Biol Med. 53:1252–1263. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Leadsham JE, Sanders G, Giannaki S, Bastow EL, Hutton R, Naeimi WR, Breitenbach M and Gourlay CW: Loss of cytochrome c oxidase promotes RAS-dependent ROS production from the ER resident NADPH oxidase, Yno1p, in yeast. Cell Metab. 18:279–286. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Zou H, Li Y, Liu X and Wang X: An APAF-1. cytochrome c multimeric complex is a functional apoptosome that activates procaspase-9. J Biol Chem. 274:11549–11556. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Hu Y, Benedict MA, Ding L and Núñez G: Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. EMBO J. 18:3586–3595. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Saleh A, Srinivasula SM, Acharya S, Fishel R and Alnemri ES: Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J Biol Chem. 274:17941–17945. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Hassanin IM, Shahin AY, Badawy MS and Karam K: D-dimer testing versus multislice computed tomography in the diagnosis of postpartum pulmonary embolism in symptomatic high-risk women. Int J Gynaecol Obstet. 115:200–201. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Glerum DM, Shtanko A and Tzagoloff A: Characterization of COX17, a yeast gene involved in copper metabolism and assembly of cytochrome oxidase. J Biol Chem. 271:14504–14509. 1996. View Article : Google Scholar : PubMed/NCBI

24 

Takahashi Y, Kako K, Kashiwabara S, Takehara A, Inada Y, Arai H, Nakada K, Kodama H, Hayashi J, Baba T and Munekata E: Mammalian copper chaperone Cox17p has an essential role in activation of cytochrome C oxidase and embryonic development. Mol Cell Biol. 22:7614–7621. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yang F, Qi X, Gao Z, Yang X, Zheng X, Duan C and Zheng J: Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity. Exp Ther Med 12: 2247-2252, 2016.
APA
Yang, F., Qi, X., Gao, Z., Yang, X., Zheng, X., Duan, C., & Zheng, J. (2016). Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity. Experimental and Therapeutic Medicine, 12, 2247-2252. https://doi.org/10.3892/etm.2016.3564
MLA
Yang, F., Qi, X., Gao, Z., Yang, X., Zheng, X., Duan, C., Zheng, J."Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity". Experimental and Therapeutic Medicine 12.4 (2016): 2247-2252.
Chicago
Yang, F., Qi, X., Gao, Z., Yang, X., Zheng, X., Duan, C., Zheng, J."Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2247-2252. https://doi.org/10.3892/etm.2016.3564
Copy and paste a formatted citation
x
Spandidos Publications style
Yang F, Qi X, Gao Z, Yang X, Zheng X, Duan C and Zheng J: Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity. Exp Ther Med 12: 2247-2252, 2016.
APA
Yang, F., Qi, X., Gao, Z., Yang, X., Zheng, X., Duan, C., & Zheng, J. (2016). Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity. Experimental and Therapeutic Medicine, 12, 2247-2252. https://doi.org/10.3892/etm.2016.3564
MLA
Yang, F., Qi, X., Gao, Z., Yang, X., Zheng, X., Duan, C., Zheng, J."Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity". Experimental and Therapeutic Medicine 12.4 (2016): 2247-2252.
Chicago
Yang, F., Qi, X., Gao, Z., Yang, X., Zheng, X., Duan, C., Zheng, J."Homocysteine injures vascular endothelial cells by inhibiting mitochondrial activity". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2247-2252. https://doi.org/10.3892/etm.2016.3564
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team