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Article Open Access

Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells

Retraction in: /10.3892/etm.2021.10220
  • Authors:
    • Hongyi Tan
    • Xiaoshan Wang
    • Xiaogang Yang
    • Haitao Li
    • Ben Liu
    • Pinhua Pan
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China, Department of Oncology, The Third Hospital of Changsha, Changsha, Hunan 410015, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2088-2094
    |
    Published online on: August 10, 2016
       https://doi.org/10.3892/etm.2016.3584
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Abstract

Lung adenocarcinoma, which is the most common non-small cell lung cancer, is the leading cause of death from cancer worldwide. Epithelial cell transforming sequence 2 (ECT2) is frequently upregulated and acts as an oncogene in various human cancers. In addition, ECT2 was reported to be upregulated in early stage lung adenocarcinoma. However, the detailed role of ECT2 in mediating the malignant phenotypes of lung adenocarcinoma cells has not previously been elucidated. Reverse transcription‑quantitative polymerase chain reaction and western blot analysis were used to examine ECT2 mRNA and protein expression levels, respectively. MTT, wound healing and Transwell assays were conducted to determine cell proliferation, migration and invasion abilities, respectively. In the present study, ECT2 was significantly upregulated in lung adenocarcinoma cell lines (H650, EKVX, HCC4006, HCC827, HCC2935, Hop62 and A549), as compared with a normal lung epithelial cell line (BEAS‑2B). Moreover, knockdown of ECT2, induced by transfection with ECT2 siRNA, significantly inhibited the proliferation of lung adenocarcinoma A549 cells, whereas overexpression of ECT2 enhanced A549 cell proliferation. Furthermore, knockdown of ECT2 expression suppressed the migration and invasion of A549 cells, whereas overexpression of ECT2 enhanced the migration and invasion abilities of A549 cells. Notably, inhibition of ECT2 also suppressed the expression levels of N‑cadherin and vimentin, whereas it enhanced the expression level of E‑cadherin, indicating that ECT2 is associated with the epithelial‑mesenchymal transition in A549 cells. On the contrary, overexpression of ECT2 enhanced the expression levels of N‑cadherin and vimentin, whereas it reduced the expression level of E‑cadherin in A549 cells. In conclusion, the results of the present study suggest that ECT2 has an oncogenic role in lung adenocarcinoma cells. Therefore, ECT2 may be a potential novel target for the treatment of lung adenocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Tan H, Wang X, Yang X, Li H, Liu B and Pan P: Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220. Exp Ther Med 12: 2088-2094, 2016.
APA
Tan, H., Wang, X., Yang, X., Li, H., Liu, B., & Pan, P. (2016). Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220. Experimental and Therapeutic Medicine, 12, 2088-2094. https://doi.org/10.3892/etm.2016.3584
MLA
Tan, H., Wang, X., Yang, X., Li, H., Liu, B., Pan, P."Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220". Experimental and Therapeutic Medicine 12.4 (2016): 2088-2094.
Chicago
Tan, H., Wang, X., Yang, X., Li, H., Liu, B., Pan, P."Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2088-2094. https://doi.org/10.3892/etm.2016.3584
Copy and paste a formatted citation
x
Spandidos Publications style
Tan H, Wang X, Yang X, Li H, Liu B and Pan P: Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220. Exp Ther Med 12: 2088-2094, 2016.
APA
Tan, H., Wang, X., Yang, X., Li, H., Liu, B., & Pan, P. (2016). Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220. Experimental and Therapeutic Medicine, 12, 2088-2094. https://doi.org/10.3892/etm.2016.3584
MLA
Tan, H., Wang, X., Yang, X., Li, H., Liu, B., Pan, P."Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220". Experimental and Therapeutic Medicine 12.4 (2016): 2088-2094.
Chicago
Tan, H., Wang, X., Yang, X., Li, H., Liu, B., Pan, P."Oncogenic role of epithelial cell transforming sequence 2 in lung adenocarcinoma cells Retraction in /10.3892/etm.2021.10220". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2088-2094. https://doi.org/10.3892/etm.2016.3584
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