Open Access

Reversal effect and mechanism of Ginkgo biloba exocarp extracts in multidrug resistance of mice S180 tumor cells

  • Authors:
    • Bi‑Yuan Hu
    • Yun‑Hao Gu
    • Chen‑Jie Cao
    • Jun Wang
    • Dong‑Dong Han
    • Ying‑Chao Tang
    • Hua‑Sheng Chen
    • Aihua Xu
  • View Affiliations

  • Published online on: August 11, 2016     https://doi.org/10.3892/etm.2016.3589
  • Pages: 2053-2062
  • Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study was to investigate the reversal effect and its related mechanism of Ginkgo biloba exocarp extracts (GBEEs) in obtained multidrug resistance (MDR) of mice S180 tumor cells in vitro and in vivo. In order to simulate the clinical PFC [cis‑dichlorodiamineplatinum, cisplatin (DDP) + fluorouracil (FU), FU+cyclophosphamide and cyclophosphamide] scheme, a gradually increasing dose was administered in a phased induction in order to induce S180 cells in vivo and to make them obtain multidrug resistance. The results in vitro demonstrated that GBEE could significantly increase the IC50 of DDP on S180 MDR cells, increase the accumulation of Adriamycin (ADR) and rhodamine 123 (Rho 123), and reduce the efflux of Rho 123 of S180 MDR cells. The results from the in vivo treatment with a combination of GBEE and DDP to S180 MDR ascites tumor in mice demonstrated that each dose of GBEE could effectively reverse the drug‑resistance of S180 MDR cells to DDP in order to extend the survival time of mice with ascite tumors and inhibit tumor growth in solid tumor mice. In addition, GBEE effectively inhibited the expression of MDR‑1 mRNA and multidrug resistance‑associated protein‑1 mRNA in S180 MDR cells of ascites tumor in mice and improved the expression levels of cytokines, including interleukin (IL)‑3, IL‑18 and interferon‑γ in the blood serum of S180 MDR tumor‑bearing mice. The present study showed that the mechanism of GBEE reversal of MDR may be associated with the inhibition of the functional activity of P‑glycoprotein, the downregulation of drug resistance related gene expression of S180 MDR cells and the improvement of the production of related serum cytokines of S180 MDR tumor mice.
View Figures
View References

Related Articles

Journal Cover

October-2016
Volume 12 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hu BY, Gu YH, Cao CJ, Wang J, Han DD, Tang YC, Chen HS and Xu A: Reversal effect and mechanism of Ginkgo biloba exocarp extracts in multidrug resistance of mice S180 tumor cells. Exp Ther Med 12: 2053-2062, 2016
APA
Hu, B., Gu, Y., Cao, C., Wang, J., Han, D., Tang, Y. ... Xu, A. (2016). Reversal effect and mechanism of Ginkgo biloba exocarp extracts in multidrug resistance of mice S180 tumor cells. Experimental and Therapeutic Medicine, 12, 2053-2062. https://doi.org/10.3892/etm.2016.3589
MLA
Hu, B., Gu, Y., Cao, C., Wang, J., Han, D., Tang, Y., Chen, H., Xu, A."Reversal effect and mechanism of Ginkgo biloba exocarp extracts in multidrug resistance of mice S180 tumor cells". Experimental and Therapeutic Medicine 12.4 (2016): 2053-2062.
Chicago
Hu, B., Gu, Y., Cao, C., Wang, J., Han, D., Tang, Y., Chen, H., Xu, A."Reversal effect and mechanism of Ginkgo biloba exocarp extracts in multidrug resistance of mice S180 tumor cells". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2053-2062. https://doi.org/10.3892/etm.2016.3589