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Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study

  • Authors:
    • Serkan Dundar
    • Cem Ozgur
    • Ferhan Yaman
    • Omer Cakmak
    • Arif Saybak
    • Ibrahim Hanifi Ozercan
    • Hilal Alan
    • Gokhan Artas
    • Onur Nacakgedigi
  • View Affiliations / Copyright

    Affiliations: Department of Periodontology, Faculty of Dentistry, Firat University, Elazığ, 23119 Elazığ, Turkey, Department of Oral Maxillofacial Surgery, Corlu Dental and Oral Health Centre, Ministry of Health, Tekirdağ, 59850 Tekirdağ, Turkey, Department of Oral Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakır, 21280 Diyarbakır, Turkey, Department of Periodontology, Faculty of Dentistry, Afyon Kocatepe University, Afyonkarahisar, 03200 Afyon, Turkey, Private Practice, Adana, 01220 Adana, Turkey, Department of Medical Pathology, Faculty of Medicine, Firat University, Elazığ, 23119 Elazığ, Turkey, Department of Oral Maxillofacial Surgery, Faculty of Dentistry, Inonu University, Malatya, 44280 Malatya, Turkey, Department of Restorative Dentistry, Dicle University, Diyarbakır, 21280 Diyarbakır, Turkey
    Copyright: © Dundar et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2015-2020
    |
    Published online on: August 12, 2016
       https://doi.org/10.3892/etm.2016.3598
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Abstract

The aim of this study was to evaluate the effects on new bone formation of autogenous blood alone or in combination with zoledronic acid (ZA), a β‑tricalcium phosphate (β‑TCP) graft or ZA plus a β‑TCP graft placed under titanium barriers. For this purpose, eight adult male New Zealand white rabbits were used in the study, each with four titanium barriers fixed around four sets of nine holes drilled in the calvarial bones. The study included four groups, each containing 2 rabbits. In the autogenous blood (AB group), only autogeneous blood was placed under the titanium barriers. The three experimental groups were the AB+ZA group, with autogenous blood plus ZA, the AB+β‑TCP group, with autogeneous blood plus a β‑TCP graft, and the AB+β‑TCP+ZA group, with autogeneous blood plus a β‑TCP graft and ZA mixture under the titanium barriers. The animals were sacrificed after 3 months. The amounts of new bone formation identified histomorphometrically were found to be higher after 3 months than at the time of surgery in all groups. The differences between the groups were examined with histomorphometric analysis, and statistically significant differences were identified at the end of the 3 months. The bone formation rate in the AB+β‑TCP+ZA group was determined to be significantly higher than that in the other groups (P<0.05). In the AB+ZA and AB+β‑TCP groups, the bone formation rate was determined to be significantly higher than that in the AB group (P<0.05). No statistically significant difference in bone formation rate was observed between the AB+β‑TCP and AB+ZA groups. Local ZA used with autogeneous blood and/or graft material appears to be a more effective method than the use of autogeneous blood or graft alone in bone augmentation executed with a titanium barrier.
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1 

Buser D, Brägger U, Lang NP and Nyman S: Regeneration and enlargement of jaw bone using guided tissue regeneration. Clin Oral Implants Res. 1:22–32. 1990. View Article : Google Scholar : PubMed/NCBI

2 

Schwarz F, Herten M, Ferrari D, Wieland M, Schmitz L, Engelhardt E and Becker J: Guided bone regeneration at dehiscence-type defects using biphasic hydroxyapatite+beta tricalcium phosphate (Bone Ceramic) or a collagen-coated natural bone mineral (BioOss Collagen): An immunohistochemical study in dogs. Int J Oral Maxillofac Surg. 36:1198–1206. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Ezirganli S, Polat S, Baris E, Tatar I and Celik HH: Comparative investigation of the effects of different materials used with a titanium barrier on new bone formation. Clin Oral Implants Res. 24:312–319. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Alam S, Ueki K, Nakagawa K, Marukawa K, Hashiba Y, Yamamoto E, Sakulsak N and Iseki S: Statin-induced bone morphogenetic protein (BMP) 2 expression during bone regeneration: An immunohistochemical study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 107:22–29. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Toker H, Ozdemir H, Ozer H and Eren K: A comparative evaluation of the systemic and local alendronate treatment in synthetic bone graft: A histologic and histomorphometric study in a rat calvarial defect model. Oral Surg Oral Med Oral Pathol Oral Radiol. 114(5 Suppl): S146–S152. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Van Steenberghe D, Johansson C, Quirynen M, Molly L, Albrektsson T and Naert I: Bone augmentation by means of a stiff occlusive titanium barrier. Clin Oral Implants Res. 14:63–71. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Min S, Sato S, Saito M, Ebihara H, Arai Y and Ito K: Micro-computerized tomography analysis: Dynamics of bone augmentation within a titanium cap in rabbit calvarium. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 106:892–895. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Murai M, Sato S, Koshi R, Yokoyama K, Ikeda K, Narukawa M, Takayama T, Yoshinuma N and Ito K: Effects of the enamel matrix derivative and beta-tricalcium phosphate on bone augmentation within a titanium cap in rabbit calvarium. J Oral Sci. 47:209–217. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Molly L, Quirynen M, Michiels K and van Steenberghe D: Comparison between jaw bone augmentation by means of a stiff occlusive titanium membrane or an autologous hip graft: A retrospective clinical assessment. Clin Oral Implants Res. 17:481–487. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Doggrell SA: Clinical efficacy and safety of zoledronic acid in prostate and breast cancer. Expert Rev Anticancer Ther. 9:1211–1218. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Lipton A: The safety of zoledronic acid. Expert Opin Drug Saf. 6:305–313. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Abtahi J, Tengvall P and Aspenberg P: Bisphosphonate coating might improve fixation of dental implants in the maxilla: A pilot study. Int J Oral Maxillofac Surg. 39:673–677. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Friedl G, Radl R, Stihsen C, Rehak P, Aigner R and Windhager R: The effect of a single infusion of zoledronic acid on early implant migration in total hip arthroplasty. A randomized, double-blind, controlled trial. J Bone Joint Surg Am. 91:274–281. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Abtahi J, Agholme F, Sandberg O and Aspenberg P: Effect of local vs. systemic bisphosphonate delivery on dental implant fixation in a model of osteonecrosis of the jaw. J Dent Res. 92:279–283. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Ayan M, Dolanmaz D, Mihmanli A, Ayan A and Kürkcü M: The effect of systemically administrated zoledronic acid on the osseointegration of dental implants. Oral Dis. 18:802–808. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Back DA, Pauly S, Rommel L, Haas NP, Schmidmaier G, Wildemann B and Greiner SH: Effect of local zoledronate on implant osseointegration in a rat model. BMC Musculoskelet Disord. 13:422012. View Article : Google Scholar : PubMed/NCBI

17 

Ozdemir H, Ezirganli S, Kara M Isa, Mihmanli A and Baris E: Effects of platelet rich fibrin alone used with rigid titanium barrier. Arch Oral Biol. 58:537–544. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Greenstein G and Carpentieri JR: Utilization of d-PTFE barriers for post-extraction bone regeneration in preparation for dental implants. Compend Contin Educ Dent. 36:465–473. 2015.PubMed/NCBI

19 

Gardin C, Ricci S, Ferroni L, Guazzo R, Sbricoli L, De Benedictis G, Finotti L, Isola M, Bressan E and Zavan B: Decellularization and delipidation protocols of bovine bone and pericardium for bone grafting and guided bone regeneration procedures. PLoS One. 10:e01323442015. View Article : Google Scholar : PubMed/NCBI

20 

Smiler D and Soltan M: The bone-grafting decision tree: A systematic methodology for achieving new bone. Implant Dent. 15:122–128. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Maréchal M, Luyten F, Nijs J, Postnov A, Schepers E and van Steenberghe D: Histomorphometry and micro-computed tomography of bone augmentation under a titanium membrane. Clin Oral Implants Res. 16:708–714. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Schmid J, Hämmerle CH, Olah AJ and Lang NP: Membrane permeability is unnecessary for guided generation of new bone. An experimental study in the rabbit. Clin Oral Implants Res. 5:125–130. 1994. View Article : Google Scholar : PubMed/NCBI

23 

Martinez A, Balboa O, Gasamans I, Otero-Cepeda XL and Guitian F: Deproteinated bovine bone vs. beta-tricalcium phosphate as bone graft substitutes: Histomorphometric longitudinal study in the rabbit cranial vault. Clin Oral Implants Res. 26:623–632. 2015. View Article : Google Scholar : PubMed/NCBI

24 

Daculsi G, Laboux O, Malard O and Weiss P: Current state of the art of biphasic calcium phosphate bioceramics. J Mater Sci Mater Med. 14:195–200. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Samavedi S, Whittington AR and Goldstein AS: Calcium phosphate ceramics in bone tissue engineering: A review of properties and their influence on cell behavior. Acta Biomater. 9:8037–8045. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Nishimura I, Shimizu Y and Ooya K: Effects of cortical bone perforation on experimental guided bone regeneration. Clin Oral Implants Res. 15:293–300. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Buser D, Dula K, Belser UC, Hirt HP and Berthold H: Localized ridge augmentation using guided bone regeneration. II. Surgical procedure in the mandible. Int J Periodontics Restorative Dent. 15:10–29. 1995.PubMed/NCBI

28 

Ito K, Minegishi T, Takayama T, Tamura T, Yamada Y and Sato S: Effects of ipriflavone on augmented bone using a guided bone regeneration procedure. Clin Oral Implants Res. 18:60–68. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Lundgren AK, Lundgren D, Hämmerle CH, Nyman S and Sennerby L: Influence of decortication of the donor bone on guided bone augmentation. An experimental study in the rabbit skull bone. Clin Oral Implants Res. 11:99–106. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Lundgren AK, Lundgren D, Wennerberg A, Hammerle CH and Nyman S: Influence of surface roughness of barrier walls on guided bone augmentation: Experimental study in rabbits. Clin Implant Dent Relat Res. 1:41–48. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Linde A, Thorén C, Dahlin C and Sandberg E: Creation of new bone by an osteopromotive membrane technique: An experimental study in rats. J Oral Maxillofac Surg. 51:892–897. 1993. View Article : Google Scholar : PubMed/NCBI

32 

Rompen EH, Biewer R, Vanheusden A, Zahedi S and Nusgens B: The influence of cortical perforations and of space filling with peripheral blood on the kinetics of guided bone generation. A comparative histometric study in the rat. Clin Oral Implants Res. 10:85–94. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Schmid J, Wallkamm B, Hämmerle CH, Gogolewski S and Lang NP: The significance of angiogenesis in guided bone regeneration. A case report of a rabbit experiment. Clin Oral Implants Res. 8:244–248. 1997. View Article : Google Scholar : PubMed/NCBI

34 

Toker H, Ozdemir H, Ozer H and Eren K: Alendronate enhances osseous healing in a rat calvarial defect model. Arch Oral Biol. 57:1545–1550. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Dundar S, Ozgur C, Yaman F, Cakmak O, Saybak A, Ozercan IH, Alan H, Artas G and Nacakgedigi O: Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study. Exp Ther Med 12: 2015-2020, 2016.
APA
Dundar, S., Ozgur, C., Yaman, F., Cakmak, O., Saybak, A., Ozercan, I.H. ... Nacakgedigi, O. (2016). Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study. Experimental and Therapeutic Medicine, 12, 2015-2020. https://doi.org/10.3892/etm.2016.3598
MLA
Dundar, S., Ozgur, C., Yaman, F., Cakmak, O., Saybak, A., Ozercan, I. H., Alan, H., Artas, G., Nacakgedigi, O."Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study". Experimental and Therapeutic Medicine 12.4 (2016): 2015-2020.
Chicago
Dundar, S., Ozgur, C., Yaman, F., Cakmak, O., Saybak, A., Ozercan, I. H., Alan, H., Artas, G., Nacakgedigi, O."Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2015-2020. https://doi.org/10.3892/etm.2016.3598
Copy and paste a formatted citation
x
Spandidos Publications style
Dundar S, Ozgur C, Yaman F, Cakmak O, Saybak A, Ozercan IH, Alan H, Artas G and Nacakgedigi O: Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study. Exp Ther Med 12: 2015-2020, 2016.
APA
Dundar, S., Ozgur, C., Yaman, F., Cakmak, O., Saybak, A., Ozercan, I.H. ... Nacakgedigi, O. (2016). Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study. Experimental and Therapeutic Medicine, 12, 2015-2020. https://doi.org/10.3892/etm.2016.3598
MLA
Dundar, S., Ozgur, C., Yaman, F., Cakmak, O., Saybak, A., Ozercan, I. H., Alan, H., Artas, G., Nacakgedigi, O."Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study". Experimental and Therapeutic Medicine 12.4 (2016): 2015-2020.
Chicago
Dundar, S., Ozgur, C., Yaman, F., Cakmak, O., Saybak, A., Ozercan, I. H., Alan, H., Artas, G., Nacakgedigi, O."Guided bone regeneration with local zoledronic acid and titanium barrier: An experimental study". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2015-2020. https://doi.org/10.3892/etm.2016.3598
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