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Article

Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries

  • Authors:
    • Yulong Chen
    • Cangbao Xu
    • Yahui Wei
    • Yaping Zhang
    • Ailan Cao
  • View Affiliations / Copyright

    Affiliations: Shaanxi Pharmaceutical Development Center, Shaanxi Pharmaceutical Holding Group Co., Ltd., Xi'an, Shaanxi 710075, P.R. China, Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. China, Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, P.R. China
  • Pages: 2241-2246
    |
    Published online on: August 22, 2016
       https://doi.org/10.3892/etm.2016.3605
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Abstract

Resveratrol (Res) is a specific agonist of sirtuin 1, and has many cardioprotective effects. Although Res is able to relax various vascular beds, its pharmacological properties in rat superior mesenteric arteries and the underlying mechanism are not well clarified. The aim of present study was to investigate the vasorelaxant effects of Res on rat superior mesenteric arteries and the mechanisms involved. The isometric tension of rat superior mesenteric arterial rings was recorded in vitro using myography. It was found that Res concentration‑dependently relaxed endothelium‑intact superior mesenteric artery rings pre‑contracted by phenylephrine hydrochloride (Emax, 97.66±0.79%; pD2, 4.30±0.14) or KCl (Emax, 101.3±0.6%; pD2, 4.12±0.03). The vasorelaxant effect of Res on the superior mesenteric artery rings was partially endothelium-dependent. NG-nitro-L‑arginine methyl ester (100 µM) significantly inhibited the Res‑induced vasorelaxant effect. However, 1H-[1,2,4]oxadiazolo[4,3‑a] quinoxalin‑1‑one (10 µM) and indomethacin (5 µM) each had no effect on the Res‑induced vasorelaxation. In artery rings without endothelium, the vasorelaxation induced by Res was attenuated by 4‑aminopyridine (100 µM) and glibenclamide (10 µM). However, barium chloride dehydrate (10 µM) and tetraethylammonium chloride (1 mM) did not affect the vasorelaxation induced by Res. Moreover, Res also inhibited the contraction induced by an increase in external calcium concentration in Ca2+‑free medium plus KCl (60 mM). These results suggest that Res induces relaxation in superior mesenteric arterial rings through an endothelium‑dependent pathway, involving nitric oxide release, and also through an endothelium‑independent pathway, with opening of voltage‑dependent K+ channels and ATP‑sensitive K+ channels and blockade of extracellular Ca2+ influx.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Xu C, Wei Y, Zhang Y and Cao A: Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries. Exp Ther Med 12: 2241-2246, 2016.
APA
Chen, Y., Xu, C., Wei, Y., Zhang, Y., & Cao, A. (2016). Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries. Experimental and Therapeutic Medicine, 12, 2241-2246. https://doi.org/10.3892/etm.2016.3605
MLA
Chen, Y., Xu, C., Wei, Y., Zhang, Y., Cao, A."Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries". Experimental and Therapeutic Medicine 12.4 (2016): 2241-2246.
Chicago
Chen, Y., Xu, C., Wei, Y., Zhang, Y., Cao, A."Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2241-2246. https://doi.org/10.3892/etm.2016.3605
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Xu C, Wei Y, Zhang Y and Cao A: Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries. Exp Ther Med 12: 2241-2246, 2016.
APA
Chen, Y., Xu, C., Wei, Y., Zhang, Y., & Cao, A. (2016). Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries. Experimental and Therapeutic Medicine, 12, 2241-2246. https://doi.org/10.3892/etm.2016.3605
MLA
Chen, Y., Xu, C., Wei, Y., Zhang, Y., Cao, A."Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries". Experimental and Therapeutic Medicine 12.4 (2016): 2241-2246.
Chicago
Chen, Y., Xu, C., Wei, Y., Zhang, Y., Cao, A."Endothelium-dependent and-independent relaxation induced by resveratrol in rat superior mesenteric arteries". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2241-2246. https://doi.org/10.3892/etm.2016.3605
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