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Article

Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway

  • Authors:
    • Zhiyong Cai
    • Jindi Liu
    • Hongliang Bian
    • Jinlan Cai
  • View Affiliations / Copyright

    Affiliations: Newborn Department, Yancheng Maternity and Child Health Care Hospital, Yancheng, Jiangsu 224000, P.R. China
  • Pages: 2702-2708
    |
    Published online on: August 30, 2016
       https://doi.org/10.3892/etm.2016.3629
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Abstract

Astragaloside IV (AS‑IV) is a flavonoid from the plant Astragalus membranaceus (Fisch) Bge that has a wide range of therapeutic effects. The aim of the present study was to examine the effect of AS‑IV on rats with necrotizing enterocolitis (NEC) under oxidative stress and inflammation. Newborn Sprague‑Dawley rats were induced with NEC by asphyxia and hypothermia applied on 3 consecutive days. The rats were orally administered AS‑IV at 25, 50 and 75 mg/kg for 4 days. The results revealed that AS‑IV administration prevented NEC‑induced decrease in the concentration of malondialdehyde and myeloperoxidase, and increase in the activity of glutathione (GSH) and superoxide dismutase in murine models. AS‑IV also inhibited NEC‑induced elevation in the levels of interleukin (IL)­‑6, IL‑1β, tumor necrosis factor‑α and nuclear factor (NF)‑κB. The effects of AS‑IV were achieved under inflammation and oxidative stress. Western blotting demonstrated that AS‑IV substantially inhibited the phosphorylated (p)‑IκBα, NF‑κBp65, p‑NF‑κBp65 protein levels and increased vitamin D3 upregulated protein 1 (VDUP1) and IκBα protein levels. These data indicate that AS‑IV may be effective in the protection of NEC‑induced ileum degeneration by inhibiting the levels of inflammatory markers and oxidative stress via the regulation of the VDUP1/NF‑κB signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Cai Z, Liu J, Bian H and Cai J: Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway. Exp Ther Med 12: 2702-2708, 2016.
APA
Cai, Z., Liu, J., Bian, H., & Cai, J. (2016). Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway. Experimental and Therapeutic Medicine, 12, 2702-2708. https://doi.org/10.3892/etm.2016.3629
MLA
Cai, Z., Liu, J., Bian, H., Cai, J."Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway". Experimental and Therapeutic Medicine 12.4 (2016): 2702-2708.
Chicago
Cai, Z., Liu, J., Bian, H., Cai, J."Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2702-2708. https://doi.org/10.3892/etm.2016.3629
Copy and paste a formatted citation
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Spandidos Publications style
Cai Z, Liu J, Bian H and Cai J: Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway. Exp Ther Med 12: 2702-2708, 2016.
APA
Cai, Z., Liu, J., Bian, H., & Cai, J. (2016). Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway. Experimental and Therapeutic Medicine, 12, 2702-2708. https://doi.org/10.3892/etm.2016.3629
MLA
Cai, Z., Liu, J., Bian, H., Cai, J."Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway". Experimental and Therapeutic Medicine 12.4 (2016): 2702-2708.
Chicago
Cai, Z., Liu, J., Bian, H., Cai, J."Astragaloside IV ameliorates necrotizing enterocolitis by attenuating oxidative stress and suppressing inflammation via the vitamin D3-upregulated protein 1/NF-κB signaling pathway". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2702-2708. https://doi.org/10.3892/etm.2016.3629
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