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TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons

  • Authors:
    • Qi‑Shan Zhang
    • Yuan‑Gao Liao
    • Zhong Ji
    • Yong Gu
    • Hai‑Shan Jiang
    • Zuo‑Shan Xie
    • Su‑Yue Pan
    • Ya‑Fang Hu
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2594-2598
    |
    Published online on: September 5, 2016
       https://doi.org/10.3892/etm.2016.3658
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Abstract

The present study aimed to investigate the protective effect of a modified p5 peptide, TFP5, on 1-methyl-4-phenyl pyridine ion (MPP+)-induced neurotoxicity in cortical neurons and explore the therapeutic effect of TFP5 on Parkinson's disease (PD). MPP+ was applied to a primary culture of mouse cortical neurons to establish the cell model of PD. Neurons were divided into four groups: Control, model (MPP+), scrambled peptide (Scb) (Scb + MPP+) and TFP5 (TFP5 + MPP+) groups. Pretreatment with Scb or TFP5 was applied to the latter two groups, respectively, for 3 h, while phosphate‑buffered saline was applied to the control and model groups. MPP+ was then applied to all groups, with the exception of the control group, and neurons were cultured for an additional 24 h. Neuron viability was evaluated using a Cell Counting kit‑8 (CCK8) assay. To explore the mechanism underlying the protective effects of TFP5, the expression levels of p35, p25 and phosphorylated myocyte enhancer factor 2 (p‑MEF2D) were determined by western blotting. Fluorescence microscopy showed that TFP5 was able to pass through cell membranes and distribute around the nucleus. CCK8 assay showed that neuronal apoptosis was dependent on MPP+ concentration and exposure time. Cell viability decreased significantly in the model group compared with the control group (55±7 vs. 100±0%; P<0.01), and increased significantly in the TFP5 group compared with the model group (98±2 vs. 55±5%; P<0.01) and Scb group (98±2 vs. 54±4%; P<0.01). Scb exhibited no protective effect. Western blotting results showed that MPP+ induced p25 and p‑MEF2D expression, TFP5 and Scb did not affect MPP+‑induced p25 expression, but TFP5 reduced MPP+‑induced p‑MEF2D expression. In summary, TFP5 protects against MPP+‑induced neurotoxicity in mouse cortical neurons, possibly through inhibiting the MPP+‑induced formation and elevated kinase activity of a cyclin‑dependent kinase 5/p25 complex.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang QS, Liao YG, Ji Z, Gu Y, Jiang HS, Xie ZS, Pan SY and Hu YF: TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons. Exp Ther Med 12: 2594-2598, 2016.
APA
Zhang, Q., Liao, Y., Ji, Z., Gu, Y., Jiang, H., Xie, Z. ... Hu, Y. (2016). TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons. Experimental and Therapeutic Medicine, 12, 2594-2598. https://doi.org/10.3892/etm.2016.3658
MLA
Zhang, Q., Liao, Y., Ji, Z., Gu, Y., Jiang, H., Xie, Z., Pan, S., Hu, Y."TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons". Experimental and Therapeutic Medicine 12.4 (2016): 2594-2598.
Chicago
Zhang, Q., Liao, Y., Ji, Z., Gu, Y., Jiang, H., Xie, Z., Pan, S., Hu, Y."TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2594-2598. https://doi.org/10.3892/etm.2016.3658
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang QS, Liao YG, Ji Z, Gu Y, Jiang HS, Xie ZS, Pan SY and Hu YF: TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons. Exp Ther Med 12: 2594-2598, 2016.
APA
Zhang, Q., Liao, Y., Ji, Z., Gu, Y., Jiang, H., Xie, Z. ... Hu, Y. (2016). TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons. Experimental and Therapeutic Medicine, 12, 2594-2598. https://doi.org/10.3892/etm.2016.3658
MLA
Zhang, Q., Liao, Y., Ji, Z., Gu, Y., Jiang, H., Xie, Z., Pan, S., Hu, Y."TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons". Experimental and Therapeutic Medicine 12.4 (2016): 2594-2598.
Chicago
Zhang, Q., Liao, Y., Ji, Z., Gu, Y., Jiang, H., Xie, Z., Pan, S., Hu, Y."TFP5 prevents 1-methyl-4-phenyl pyridine ion-induced neurotoxicity in mouse cortical neurons". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2594-2598. https://doi.org/10.3892/etm.2016.3658
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