Open Access

Systemic and local zoledronic acid treatment with hydroxyapatite bone graft: A histological and histomorphometric experimental study

  • Authors:
    • Nedim Günes
    • Serkan Dundar
    • Arif Saybak
    • Gökhan Artas
    • Izzet Acikan
    • I. Hanifi Ozercan
    • Serhat Atilgan
    • Ferhan Yaman
  • View Affiliations

  • Published online on: September 7, 2016     https://doi.org/10.3892/etm.2016.3685
  • Pages: 2417-2422
  • Copyright: © Günes et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In this study, the aim was to compare the relative efficacy of systemic and local zoledronic acid (ZA) on a hydroxyapatite (HA) bone graft in a rat critical‑size calvarial bone defect. In total, 84 female rats were divided into four groups: Empty control (EC) group with no treatment applied; HA group, in which only HA bone graft material was used in the calvarium; and HA plus local ZA (HA+LZA) and HA plus systemic ZA (HA+SZA) groups, in which animals received ZA locally or systemically, respectively, with HA bone graft material in the calvarium. A 5‑mm standardised critical‑size calvarial bone defect was created with a standard trephine drill and the respective treatment was applied. Rats were sacrificed 7, 14 and 28 days later. The numbers of osteoclasts and osteoblasts, and degree of bone formation were evaluated histopathologically and histomorphometrically. Statistically significant differences were detected between the HA, HA+LZA and HA+SZA groups and the EC group for new bone formation (P<0.05). Osteoblast numbers in the HA+LZA and HA+SZA groups were significantly higher compared with those in the EC and HA groups (P<0.05). No statistically significant difference was detected between the HA+LZA and HA+SZA groups in new bone formation or osteoblast number (P>0.05). Bone formation was significantly higher in the HA group than in the EC group (P<0.05). The numbers of osteoclasts in the HA+LZA and HA+SZA groups were significantly higher than those in the groups EC and HA (P<0.05); however, there was no significant difference between groups HA+LZA and HA+SZA (P>0.05). Within the limitations of this study, systemic or local administration of ZA enhanced new bone formation with a HA bone graft in a rat critical‑size calvarial defect model.
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October-2016
Volume 12 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Günes N, Dundar S, Saybak A, Artas G, Acikan I, Ozercan IH, Atilgan S and Yaman F: Systemic and local zoledronic acid treatment with hydroxyapatite bone graft: A histological and histomorphometric experimental study. Exp Ther Med 12: 2417-2422, 2016
APA
Günes, N., Dundar, S., Saybak, A., Artas, G., Acikan, I., Ozercan, I.H. ... Yaman, F. (2016). Systemic and local zoledronic acid treatment with hydroxyapatite bone graft: A histological and histomorphometric experimental study. Experimental and Therapeutic Medicine, 12, 2417-2422. https://doi.org/10.3892/etm.2016.3685
MLA
Günes, N., Dundar, S., Saybak, A., Artas, G., Acikan, I., Ozercan, I. H., Atilgan, S., Yaman, F."Systemic and local zoledronic acid treatment with hydroxyapatite bone graft: A histological and histomorphometric experimental study". Experimental and Therapeutic Medicine 12.4 (2016): 2417-2422.
Chicago
Günes, N., Dundar, S., Saybak, A., Artas, G., Acikan, I., Ozercan, I. H., Atilgan, S., Yaman, F."Systemic and local zoledronic acid treatment with hydroxyapatite bone graft: A histological and histomorphometric experimental study". Experimental and Therapeutic Medicine 12, no. 4 (2016): 2417-2422. https://doi.org/10.3892/etm.2016.3685