Anticancer activities of alkaloids extracted from the Ba lotus seed in human nasopharyngeal carcinoma CNE‑1 cells

Zhao,Xin; Feng,Xia; Peng,Deguang; Liu,Weiwei; Sun,Peng; Li,Guijie; Gu,Lianjie; Song,Jia‑Le

doi:10.3892/etm.2016.3727

Anticancer activities of alkaloids extracted from the Ba lotus seed in human nasopharyngeal carcinoma CNE‑1 cells

Authors:
- Xin Zhao
- Xia Feng
- Deguang Peng
- Weiwei Liu
- Peng Sun
- Guijie Li
- Lianjie Gu
- Jia‑Le Song
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Affiliations: Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, P.R. China , Chongqing Enterprise Engineering Research Center of Ba‑lotus Breeding and Deep Processing, Chongqing 400041, P.R. China, School of Public Health and Management, Chongqing Medical University, Chongqing 400016, P.R. China, Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi 541004, P.R. China
Published online on: September 20, 2016 https://doi.org/10.3892/etm.2016.3727
Pages: 3113-3120

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Abstract

To investigate the anticancer activities of alkaloids from the Ba lotus seed (BLSA) in human nasopharyngeal carcinoma (NPC) CNE‑1 cells, an MTT assay, flow cytometry, reverse transcription‑polymerase chain reaction and western blotting were performed. BLSA was found to significantly reduce CNE‑1 cell proliferation in a dose‑dependent manner at all concentrations compared with the control (P<0.05). In addition, flow cytometry analysis identified that BLSA treatment significantly increased the sub‑G1 content in CNE‑1 cells (P<0.05). Following BLSA treatment, the mRNA and protein levels of a number of apoptosis‑related factors, such as caspase family members (caspase‑3, ‑8 and ‑9), B‑cell lymphoma (Bcl)‑2‑associated X protein, Fas and Fas ligand were significantly increased compared with the control (P<0.05). This was accompanied by a significant decrease in anti‑apoptotic Bcl‑2 and Bcl‑extra large protein expression compared with the control (P<0.05). Furthermore, BLSA treatment was determined to modulate CNE‑1 cell expression of nuclear factor (NF)‑κB and NF‑κB inhibitor α. The results of the present study indicate that BLSA has anticancer activity through inducing cellular apoptosis. In addition, these results suggest that BLSA can be used as a therapeutic agent in NPC.

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