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Article

Role of microRNA-141 in colorectal cancer with lymph node metastasis

  • Authors:
    • Li Feng
    • Hongqing Ma
    • Liang Chang
    • Xinliang Zhou
    • Na Wang
    • Lianmei Zhao
    • Jing Zuo
    • Yudong Wang
    • Jing Han
    • Guiying Wang
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Second Department of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Department of Molecular Biology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
  • Pages: 3405-3410
    |
    Published online on: September 27, 2016
       https://doi.org/10.3892/etm.2016.3751
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Abstract

The present study aimed to investigate the role of microRNA (miR)‑141 in the pathogenesis of colorectal cancer (CRC). In total, 58 CRC patients were included in the present study. The mRNA and protein expression levels of mitogen-activated protein kinase 4 (MAP4K4) were detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) and western blot analysis, respectively. The miRNA‑141 expression was measured by RT‑qPCR, while serum MAP4K4 content was detected by enzyme‑linked immunosorbent assay. Natural killer (NK) cells and T cells in peripheral blood were detected by flow cytometry. The results indicated that the mRNA and protein expression levels of MAP4K4 were significantly elevated in the tumor tissues, lymph nodes (P<0.01) and serum (P<0.05) in CRC. Furthermore, the expression levels of MAP4K4 in CRC patients with lymph node metastasis were higher compared with those in patients without metastasis. Bioinformatics analysis revealed that MAP4K4 may be the target gene of miRNA‑141. The expression levels of miRNA‑141 in the tumor tissues, lymph nodes and serum were significantly decreased in CRC patients, with a more evident decline in cases with lymph node metastasis. In addition, the percentage of NK, CD3+ T and CD4+ T cells was significantly decreased, whilst the number of CD8+ T cells was significantly increased, in the peripheral blood in CRC. The present results showed that miRNA‑141 was downregulated in CRC, which increased the expression levels of MAP4K4 and altered the anti‑tumor response, further increasing the proliferation, invasion and metastasis of the tumors. These findings may contribute to improving the current understanding of the pathogenesis of CRC, and lead to the development of therapies involving miRNA-141.
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1 

Wan DS: Epidemiological trend and control strategy of colorectal cancer in China. Zhong Hua Zhong Liu Za Zhi. 33:481–483. 2011.(In Chinese).

2 

Thosani N, Guha S and Singh H: Colonoscopy and colorectal cancer incidence and mortality. Gastroenterol Clin North Am. 42:619–637. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Wang Y, Chou WP, Shen CJ, Wang XY and Luo J: Risk factors of postoperative recurrence after radical resection of colon cancer. Dalian Yi Ke Da Xue Xue Bao. 35:65–67. 2013.(In Chinese).

4 

Yang S, Liu W, Li M, Wen J, Zhu M and Xu S: Insulin-like growth factor-1 modulates polycomb Cbx8 expression and inhibits colon cancer cell apoptosis. Cell Biochem Biophys. Nov 15–2014.(Epub ahead of print).

5 

Tsuchida A, Ohno S, Wu W, Borjigin N, Fujita K, Aoki T, Ueda S, Takanashi M and Kuroda M: miR-92 is a key oncogenic component of the miR-17-92 cluster in colon cancer. Cancer Sci. 102:2264–2271. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Väyrynen JP, Kantola T, Väyrynen SA, Klintrup K, Bloigu R, Karhu T, Mäkelä J, Herzig KH, Karttunen TJ, Tuomisto A and Mäkinen MJ: The relationships between serum cytokine levels and tumor infiltrating immune cells and their clinical significance in colorectal cancer. Int J Cancer. 139:112–121. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Kim JM, Cho SJ, Oh YK, Jung HY, Kim YJ and Kim N: Nuclear factor-kappa B activation pathway in intestinal epithelial cells is a major regulator of chemokine gene expression and neutrophil migration induced by Bacteroides fragilis enterotoxin. Clin Exp Immunol. 130:59–66. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Jin SH, Kim TI, Han DS, Shin SK and Kim WH: Thalidomide suppresses the interleukin 1beta-induced NFkappaB signaling pathway in colon cancer cells. Ann N Y Acad Sci. 973:414–418. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Royuela M, Rodríguez-Berriguete G, Fraile B and Paniagua R: TNF-alpha/IL-1/NF-kappaB transduction pathway in human cancer prostate. Histol Histopathol. 23:1279–1290. 2008.PubMed/NCBI

10 

Rangaswami H and Kundu GC: Osteopontin stimulates melanoma growth and lung metastasis through NIK/MEKK1-dependent MMP-9 activation pathways. Oncol Rep. 18:909–915. 2007.PubMed/NCBI

11 

Collins CS, Hong J, Sapinoso L, Zhou Y, Liu Z, Micklash K, Schultz PG and Hampton GM: A small interfering RNA screen for modulators of tumor cell motility identifies MAP4K4 as a promigratory kinase. Proc Natl Acad Sci USA. 103:3775–3780. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Wright JH, Wang X, Manning G, LaMere BJ, Le P, Zhu S, Khatry D, Flanagan PM, Buckley SD, Whyte DB, et al: The STE20 kinase HGK is broadly expressed in human tumor cells and can modulate cellular transformation, invasion and adhesion. Mol Cell Biol. 23:2068–2082. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Wang B, Shen ZL, Gao ZD, Zhao G, Wang CY, Yang Y, Zhang JZ, Yan YC, Shen C, Jiang KW, et al: MiR-194, commonly repressed in colorectal cancer, suppresses tumor growth by regulating the MAP4K4/c-Jun/MDM2 signaling pathway. Cell cycle. 14:1046–1058. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Bureau of Medical Administration, National Health; Oncology Branch of Chinese Medical Association, . Standardization of diagnosis and treatment for colorectal cancer in China (2015 edition). Zhonghua Xiaohua Waike Zazhi. 14:783–799. 2015.(In Chinese).

15 

Grimholt RM, Urdal P, Klingenberg O and Piehler AP: Rapid and reliable detection of α-globin copy number variations by quantitative real-time PCR. BMC Hematol. 14:42014. View Article : Google Scholar : PubMed/NCBI

16 

Zhao G, Wang B, Liu Y, Zhang JG, Deng SC, Qin Q, Tian K, Li X, Zhu S, Niu Y, et al: miRNA-141, downregulated in pancreatic cancer, inhibits cell proliferation and invasion by directly targeting MAP4K4. Mol Cancer Ther. 12:2569–2580. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Edwards BK, Ward E, Kohler BA, Eheman C, Zauber AG, Anderson RN, Jemal A, Schymura MJ, Lansdorp-Vogelaar I, Seeff LC, et al: Annual report to the nation on the status of cancer, 1975–2006, featuring colorectal cancer trends and impact of interventions (risk factors, screening and treatment) to reduce future rates. Cancer. 116:544–573. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Franko J, Shi Q, Goldman CD, Pockaj BA, Nelson GD, Goldberg RM, Pitot HC, Grothey A, Alberts SR and Sargent DJ: Treatment of colorectal peritoneal carcinomatosis with systemic chemotherapy: A pooled analysis of north central cancer treatment group phase III trials N9741 and N9841. J Clin Oncol. 30:263–267. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Edge SB and Compton CC: The American joint committee on cancer: The 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 17:1471–1474. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Akkoca AN, Yanik S, Ozdemir ZT, Cihan FG, Sayar S, Cincin TG, Cam A and Ozer C: TNM and modified dukes staging along with the demographic characteristics of patients with colorectal carcinoma. Int J Clin Exp Med. 7:2828–2835. 2014.PubMed/NCBI

23 

Dhillon AS, Hagan S, Rath O and Kolch W: MAP kinase signalling pathways in cancer. Oncogene. 26:3279–3290. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Chen K and Rajewsky N: The evolution of gene regulation by transcription factors and microRNAs. Nat Rev Gen. 8:93–103. 2007. View Article : Google Scholar

25 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Zhao X, Mohan R, Özcan S and Tang X: MicroRNA-30d induces insulin transcription factor MafA and insulin production by targeting mitogen-activated protein 4 kinase 4 (MAP4K4) in pancreatic β-cells. J Biol Chem. 287:31155–31164. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Iguchi N, Takeda Y, Sato N, Ukichi K, Katakura A, Ueda K, Narushima T, Higuchi S and Ogasawara K: The antihistamine olopatadine regulates T cell activation in palladium allergy. Int Immunopharmacol. 35:70–76. 2016. View Article : Google Scholar : PubMed/NCBI

28 

Quan H, Fang L, Pan H, Deng Z, Gao S, Liu O, Wang Y, Hu Y, Fang X, Yao Z, et al: An adaptive immune response driven by mature, antigen-experienced T and B cells within the microenvironment of oral squamous cell carcinoma. Int J Cancer. 138:2952–2962. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Jin B: Cell and molecular immunology. World Book Publishing Company; Beijing: 1995

30 

Bi A: Medical immunology. People's Publishing House; Beijing: 1996

31 

Wan LY, Deng J, Xiang XJ, Zhang L, Yu F, Chen J, Sun Z, Feng M and Xiong JP: miR-320 enhances the sensitivity of human colon cancer cells to chemoradiotherapy in vitro by targeting FOXM1. Biochem Biophys Res Commun. 457:125–132. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Sun Y, Xing X, Liu Q, Wang Z, Xin Y, Zhang P, Hu C and Liu Y: Hypoxia-induced autophagy reduces radiosensitivity by the HIF-1α/miR-210/Bcl-2 pathway in colon cancer cells. Int J Oncol. 46:750–756. 2015.PubMed/NCBI

33 

Tian Y, Pan Q, Shang Y, Zhu R, Ye J, Liu Y, Zhong X, Li S, He Y, Chen L, et al: MicroRNA-200 (miR-200) Cluster Regulation by Achaete Scute-like 2 (Ascl2) impact on the epithelial-mesenchymal transition in colon cancer cells. J Biol Chem. 289:36101–36115. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Feng L, Ma H, Chang L, Zhou X, Wang N, Zhao L, Zuo J, Wang Y, Han J, Wang G, Wang G, et al: Role of microRNA-141 in colorectal cancer with lymph node metastasis. Exp Ther Med 12: 3405-3410, 2016.
APA
Feng, L., Ma, H., Chang, L., Zhou, X., Wang, N., Zhao, L. ... Wang, G. (2016). Role of microRNA-141 in colorectal cancer with lymph node metastasis. Experimental and Therapeutic Medicine, 12, 3405-3410. https://doi.org/10.3892/etm.2016.3751
MLA
Feng, L., Ma, H., Chang, L., Zhou, X., Wang, N., Zhao, L., Zuo, J., Wang, Y., Han, J., Wang, G."Role of microRNA-141 in colorectal cancer with lymph node metastasis". Experimental and Therapeutic Medicine 12.5 (2016): 3405-3410.
Chicago
Feng, L., Ma, H., Chang, L., Zhou, X., Wang, N., Zhao, L., Zuo, J., Wang, Y., Han, J., Wang, G."Role of microRNA-141 in colorectal cancer with lymph node metastasis". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3405-3410. https://doi.org/10.3892/etm.2016.3751
Copy and paste a formatted citation
x
Spandidos Publications style
Feng L, Ma H, Chang L, Zhou X, Wang N, Zhao L, Zuo J, Wang Y, Han J, Wang G, Wang G, et al: Role of microRNA-141 in colorectal cancer with lymph node metastasis. Exp Ther Med 12: 3405-3410, 2016.
APA
Feng, L., Ma, H., Chang, L., Zhou, X., Wang, N., Zhao, L. ... Wang, G. (2016). Role of microRNA-141 in colorectal cancer with lymph node metastasis. Experimental and Therapeutic Medicine, 12, 3405-3410. https://doi.org/10.3892/etm.2016.3751
MLA
Feng, L., Ma, H., Chang, L., Zhou, X., Wang, N., Zhao, L., Zuo, J., Wang, Y., Han, J., Wang, G."Role of microRNA-141 in colorectal cancer with lymph node metastasis". Experimental and Therapeutic Medicine 12.5 (2016): 3405-3410.
Chicago
Feng, L., Ma, H., Chang, L., Zhou, X., Wang, N., Zhao, L., Zuo, J., Wang, Y., Han, J., Wang, G."Role of microRNA-141 in colorectal cancer with lymph node metastasis". Experimental and Therapeutic Medicine 12, no. 5 (2016): 3405-3410. https://doi.org/10.3892/etm.2016.3751
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