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Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy

  • Authors:
    • Xin-Rong Zou
    • Xiao-Qin Wang
    • Ying-Lin Hu
    • Hui-Lan Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Nephrology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430061, P.R. China
    Copyright: © Zou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3515-3520
    |
    Published online on: October 12, 2016
       https://doi.org/10.3892/etm.2016.3800
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Abstract

The effect of Shen'an granules on the Wnt signaling pathway in renal tissues of mouse models of streptozotocin (STZ)-induced diabetic nephropathy was investigated in the present study. A total of 62 BALB/c mice were randomly divided into the normal control (A group), model (B group), losartan (C group), low-dose Shen'an granules (D group), and high‑dose Shen'an granules (E group) groups. The mouse model of diabetic nephropathy was established by a single intraperitoneal injection of STZ (150 mg/kg). The animals were treated with drugs for 8 weeks, and blood creatinine, blood urea nitrogen, triglycerides (TG), and total cholesterol (CHOL) were measured prior to and after treatment. PAS staining was performed for observation of glomerular microstructure by light microscope, and western blot analysis was performed to detect Wnt1 protein and β-catenin protein. The results indicated that the quantification of 24-h microalbuminuria, and levels of blood creatinine, urea nitrogen, TG, and CHOL were significantly lower in the high- and low-dose Shen'an granules groups than those in the model group (p<0.05). The expression levels of Wnt1 protein and β-catenin protein in the high- and low-dose Shen'an granules groups were significantly lower than those in the model group (p<0.05). In conclusion, proteinuria, renal dysfunction, and dyslipidemias are closely associated with the abnormal activation of the Wnt signaling pathway in the mouse model of diabetic nephropathy. The mechanism by which Shen'an granules regulate proteinuria, renal function, and blood lipids may be associated with inhibition of the abnormally activated Wnt signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Zou X, Wang X, Hu Y and Zhou H: Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy. Exp Ther Med 12: 3515-3520, 2016.
APA
Zou, X., Wang, X., Hu, Y., & Zhou, H. (2016). Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy. Experimental and Therapeutic Medicine, 12, 3515-3520. https://doi.org/10.3892/etm.2016.3800
MLA
Zou, X., Wang, X., Hu, Y., Zhou, H."Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy". Experimental and Therapeutic Medicine 12.6 (2016): 3515-3520.
Chicago
Zou, X., Wang, X., Hu, Y., Zhou, H."Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3515-3520. https://doi.org/10.3892/etm.2016.3800
Copy and paste a formatted citation
x
Spandidos Publications style
Zou X, Wang X, Hu Y and Zhou H: Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy. Exp Ther Med 12: 3515-3520, 2016.
APA
Zou, X., Wang, X., Hu, Y., & Zhou, H. (2016). Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy. Experimental and Therapeutic Medicine, 12, 3515-3520. https://doi.org/10.3892/etm.2016.3800
MLA
Zou, X., Wang, X., Hu, Y., Zhou, H."Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy". Experimental and Therapeutic Medicine 12.6 (2016): 3515-3520.
Chicago
Zou, X., Wang, X., Hu, Y., Zhou, H."Effects of Shen'an granules on Wnt signaling pathway in mouse models of diabetic nephropathy". Experimental and Therapeutic Medicine 12, no. 6 (2016): 3515-3520. https://doi.org/10.3892/etm.2016.3800
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